افزایش پایداری غشای سلولی در گیاه توتون با انتقال ژن AtEXPB2

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجو

2 استاد/دانشگاه تهران

3 دانشجو/

4 دانشگاه تهران

چکیده

پروتئین‌های اکسپنسین‌ یکی از اجزای دیواره سلولی می‌باشند که به عنوان تنظیم‌کننده‌های کلیدی بسط و توسعه سلولی در طول رشد و نمو گیاهان شناحته می‌شوند. فعالیت اکسپنسین‌ها وابسته به pH می‌باشد. در این مطالعه گیاهان تراریخت شده با ژن AtEXPB2 از نظر برخی صفات فیزیولوژیک، بیوشیمیایی و مولکولی در شرایط بدون تنش مورد آزمایش قرارگرفتند. گیاهان مورد مطالعه شامل لاین‌های تراریخت L3، L4 و L9 به همراه گیاه شاهد بوده اند. پس از کشت بذر در محیط کشت انتخابی MS گیاهچه‌های تراریخت در خاک کشت و به گلخانه منتقل شدند. گیاهان تراریخت از نظر فعالیت آنزیم‌های آنتی‌اکسیدانی کاتالاز، گلوتاتیون‌پروکسیداز، پلی‌فنل‌اکسیداز عملکرد بهتری را نسبت به شاهد نشان دادند. همچنین لاین‌های تراریخت از نظر میزان رنگدانه‌های فتوسنتزی و پرولین نیز بر گیاه شاهد برتری داشتند. در گیاه شاهد میزان نشت یونی و پراکسیداسیون فسفولیپید‌های غشایی بیش‌تر از لاین‌های تراریخت بود. نتایج بیان نسبی ژنAtEXPB2، بیان این ژن را در گیاهان تراریخت ثابت کرده است. به صورت کلی گیاهان تراریخت در مقایسه با شاهد رفتار مناسب‌تری نشان‌دادند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Increase of cell membrane stability in tobacco plant by transferring AtEXPB2 gene

چکیده [English]

Expansin protein is a component of the cell wall generally accepted to be the key regulator of cell wall extension during plant growth. Expansins loosen the cell wall in a pH-dependent manner. In this study transgenic AtEXPB2 plants were investigated in normal growth condition. Subsequently some of their physiological, biochemical and molecular traits were measured. Plant materials were transgenic lines; L3, l4, l9 and wide type plant as control. Seeds of three transgenic lines and control were cultured in selective MS and MS medium, respectively. Then transgenic seedlings were grown in greenhouse. Transgenic plants had better antioxidant enzymatic activity of catalase, glutathione peroxidase and polyphenol oxidase as well as higher photosynthetic pigments and proline content. Control plant had higher membrane phospholipid peroxidation and electrolyte leakage. Result of relative expression of AtEXPB2 revealed that this transformed gene was being expressed in transgenic lines. Generally transgenic lines had better performance in comparison to wild type plant.

کلیدواژه‌ها [English]

  • AtEXPB2
  • Expansin
  • Cell wall extension
  • gene expression
  • Transgenic lines
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