تأثیر پوششدار کردن بذر با باکتریهای محرک رشد و عناصر ریزمغذی بر عملکرد کمی و کیفی ذرت علوفهای

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد علوم و تکنولوژی بذر، دانشگاه گیلان

2 استادیار، دانشگاه گیلان

3 استاد، دانشگاه گیلان

4 محقق، مؤسسة تحقیقات برنج رشت

چکیده

ذرت (Zea mays L.) یکی از مهمترین گیاهانی است که تولید علوفة آن در کشور رو به توسعه است. این پژوهش صحرایی به منظور بررسی تأثیر پوششدار کردن بذر با باکتریهای محرک رشد (ازتوباکتر و سودوموناس) و عناصر ریزمغذی (روی، بر، مولیبدن، مس، آهن و منگنز) بر عملکرد کمی و کیفی ذرت علوفهای رقم NS640 به اجرا درآمد. آزمایش در قالب طرح بلوکهای کامل تصادفی در سه تکرار در مؤسسة تحقیقات برنج کشور در سال 1393 انجام شد. تیمارهای مورد بررسی شامل بذر بدون پوشش، بذر پوششدار بدون باکتری و بدون عناصر ریزمغذی، پوشش بذر با عناصر ریزمغذی (روی، مولیبدن، بر، مس، آهن و منگنز) و بدون باکتری، پوشش بذر با باکتری سودوموناس و بدون عناصر ریزمغذی، پوشش بذر با باکتری سودوموناس و عناصر ریزمغذی، پوشش بذر با باکتری ازتوباکتر و بدون عناصر ریزمغذی، پوشش بذر با باکتری ازتوباکتر و عناصر ریزمغذی، پوشش بذر با مخلوطی از باکتری­های سودوموناس و ازتوباکتر و بدون عناصر ریزمغذی و پوشش بذر با مخلوطی از باکتری­های سودوموناس و ازتوباکتر و عناصر ریزمغذی بودند. تیمار پوشش بذر با باکتری سودوموناس و عناصر ریزمغذی در بیشتر صفات مورد بررسی بالاترین میزان را داشتند. از نظر کیفیت علوفه نیز، تیمار یادشده بیشترین قابلیت هضم علوفة خشک، پروتئین خام و کربوهیدرات محلول در آب را به خود اختصاص داد. بیشترین فیبر خام و خاکستر نیز در تیمار شاهد مشاهده شد. نتایج نشان داد که باکتریهای محرک رشد به همراه عناصر ریزمغذی اثربخشی بیشتری در رشد گیاه دارند. این باکتریها در تلفیق با عناصر ریزمغذی میتوانند به افزایش عملکرد کمی و کیفی گیاه منجر شوند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of seed coating with growth promoting bacteria and micronutrients on quantitative and qualitative yield of forage corn )Zea mays L. SC. 640 (

نویسندگان [English]

  • Seyed Mohammadreza Ehteshami 2
  • Jafar Asghari 3
  • Mohammad Rabiee 4
1 M. Sc. Student, University of Guilan, Iran
2 Assistant Professor, University of Guilan, Iran
3 Professor, University of Guilan, Iran
4 Researcher, Rasht Institute of Rice Research, Iran
چکیده [English]

Corn )Zea mays L. (is one of the most crop that its forage produce is developed in the country. This field research carried out in order to investigate the effect of seed coating with growth promoting bacteria )Azotobacter and Pseudomonas (and micronutrients )Zn, B, Mo, Cu, Fe and Mn (on quantitative and qualitative yield of forage corn )Zea mays L. SC. 640 (.The experiment design consisted of three randomized complete blocks was performed in 2013-2014 cropping season in Rice Researches Institute of Rasht. Investigated treatments in this research were including seed without coating, seed coating without bacteria and micronutrients, seed coating with micronutrients and without bacteria, seed coating with Pseudomonas and without micronutrients, seed coating with Pseudomonas and micronutrients, seed coating with Azotobacter and without micronutrients, seed coating with Azotobacter and micronutrients, seed coating with )Azotobacter and Pseudomonas (and without micronutrients, seed coating with )Azotobacter and Pseudomonas (and micronutrients. Seed coating with Pseudomonas and micronutrients treatment was highest in the most of characteristics. For purposes of forage quality, Seed coating with Pseudomonas and micronutrients treatment allocated the highest of dry matter digestibility percentage, crude protein percentage and water soluble carbohydrates percentage, too. Also the highest of acid detergent fiber percentage and ash percentage observed in control. The present finding showed that growth promoting bacteria with micronutrients have a greater impact on plant growth. These bacteria in combination with micronutrients can improve quantitative and qualitative yield of plant.

کلیدواژه‌ها [English]

  • Azotobacter
  • crude protein
  • Forage digestibility
  • Forage yield
  • Pseudomonas
  1. Amal, G. A., Orabi, S. & Gomaa, A. M. (2010). Bio-organic farming of grain sorghum and its effect on growth, physiological and yield parameters and antioxidant enzymes activity. Research Journal of Agriculture and Biological Sciences, 6(3), 270-279.
  2. Biscaro, G. A., Goulart Junior, S. A. R., Soratto, R. P., Freitas-Junior, N. A., Motomiya, A. V. A. & Calado-Filho, G. C. (2009). Molybdenum applied to seeds and side dressing nitrogen on irrigated common bean in cerrado soil. Science Agrotechnology, 33, 1280–1287.
  3. Becking, J. H. (2006). Prokaryotes. Chapter 3. The Family Azotobacteraceae. 6, 759-783.
  4. Chen, J. (2006). The combind use of chemical and organic fertilizers and /or biofertilizer for crop growth and soil fertility. International Workshop on Sustained Management of the Soil–Rhizosphere System for Efficient Crop Production and Fertilizer Use. 11, 16-20.
  5. Ehteshami, S. M. R., Abbasi, M. R., Khavazi, M. K. & Zand, B. (2013). Influence of different strains of Pseudomonas putida on the quantity and quality of forage sorghum cultivars in Varamin. Journal of Plant Process and Function, 2 (4), 15-26. (In Farsi)
  6. Ehteshami, S. M. R., Hakimian, F., Yousefi Rad, M. & Chaichi, M. R. (2013). Effect of Integrated management of phosphorus fertilizer on grain yield and its components in two varieties of winter barley. Pajohesh and Sazandegi, 101, 193-201. (In Farsi)
  7. Ehteshami, S. M. R., JanZamin, A., Ramezani, M., Khavazi, K. & Zand, B. (2013). Effect of integrated management of phosphorus fertilizer on quantitative and qualitative yield of two forage corn cultivars in Varamin. Journal of Crop Improvement, 15 (1), 95-110. (In Farsi)
  8. Foth, H. D. & Ellis, B. G. (1999). Soil Fertility (2th edition). Lewis publishers CRC press, Dept. MLCHIGON. 290 Pp.
  9. Farooq, M., Wahid, A. & Kadambot Siddique, H. M. (2012). Micronutrient application through seed treatments a review. Journal of Soil Science and Plant Nutrition, 12 (1), 125-142.
  10. Gad, W. M. )2001(. Physiological studies on Foeniculum vulgare and Anethum graveolense. M.Sc. Thesis. Faculty of Agriculture, Kafr ELSheikh, Tanta University, Egypt.
  11. Harris, D. (1996). The effects of manure, genotype, seed priming, depth and date of sowing on the emergence and early growth of Sorghum bicolor (L.) Moench in semi-arid Botswana. Soil and Tillage Research, 40, 73-88.
  12. Hamidi, A., Asgharzadeh, A., Choukan, R., Dehghan Shoar, M., Ghalavand, A. & Malakouti, M. J. (2007). Study on plant growth promoting rhizobacteria (PGPR) biofertilizers application in maize (Zea mays L.) cultivation by adequate input. Environmental Sciences, 4(4), 1-19.
  13. Hamidi, A. (2007). Effect of plant growth enhancer bacteria (PGPR) on grain yield and some features in maize. Journal of Agricultural Science, 37(1), 499-493. (In Farsi)
  14. Imam, y. (2003). Cereal Crops. Shiraz University Publication. (In Farsi)
  15. Jafari, A., Connolly, V., Frolich, A. & Walsh, E. K. (2003). A note on estimation of quality in perennial ryegrass by near infrared spectroscopy. Irish Journal of Agricultural and Food Research, 42, 293-299.
  16. Jafarian, V. & Lahoti, V. A. (2006). Application of water Superabsorbent polymers in projects bleaching-biological desert. Journal of Forest and Rangeland, 70: 62-58. (In Farsi)
  17. Karanam, P. V. & Vadez, V. (2010). Phosphorus coating on pearl millet seed in low P Alfisol improves plant establishment and increases stover more than seed yield. Explimentary Agriculture, 46 (4), 457-469.
  18. Kloepper, J. W., Zablowicz, R. M., Tipping, B. & Lifshitz, R. (1991). Plant growth mediated by bacterial rhizosphere colonizers. PP. 315-326. In: Keister, D. L. and B. Gregan (Eds.), The Rhizosphere and Plant Growth, BARC Symposium.
  19. Kephart, K. D., Wichman, D. M., Topinka, K. & Kirkland, K. J. (2004). Seeding date and polymer seed coating effects on plant establishment and yield of fall seeded canola in the Northern Great Plains. Canadian Journal of Plant Science, 84, 955-963.
  20. Larsen, J., Cornejo, P. & Barea, J. M. (2009). Interactions between the arbuscular mycorrhizal fungus Glomus intraradices and the plant growth promoting rhizobacteria Paenibacillus polymyxa and P. maserans in the mycorrhizosphere of Cucumis sativus. Soil Biology and Biochemistry, 41(2), 286-292.
  21. Marschner, H. (1995). Mineral Nutrition of Higher Plants, 2nd ed. Academic Press, London, UK.
  22. Masuthi, D. A., Vyakaranahal, B. S. & Deshpande, V. K. (2009). Influence of pelleting with micronutrients and botanical on growth, seed yield and quality of vegetable cowpea. Karnataka Journal of Agricultural Sciences, 22, 898–900.
  23. Martens, D. C. & Westermann, D. T. (1991). Fertilizer application for correcting micronutrient deficiencies. Micronutrients in Agriculture. Soil Science Society of America, Madison, 549-592.
  24. Mozaffari, C. & Abbasi, M. (2005). Forage crops genetic resources in National Plant Gene Bank of Iran. Proceedings of the First National Forage Crops Congress of Iran, College of Agriculture, Tehran University, Karaj, Iran. (In Farsi)
  25. Mehrabi, R., ChaiChi, M. R., Tavakol Afshari, R. Madah Arefi, H. & Zahedi Amiri, G. A. (2010). Effects of seed coating methods on seed germination of Sanguisorba minor in different soil moisture levels and sowing depths. Iranian Journal of Range and Desert Research, 17 (3), 498-489. (In Farsi)
  26. Mirshekari, B., Asadi Rahmani, H. & Mirsafari Roodsari, A. (2009). The effect of seed inoculation with Azospirillium strains and coating with micronutrients on seed yield and essence of cumin (Cuminum cyminum L.). Iranian Journal of Medicinal and Aromatic Plants, 25 (4), 471-481. (In Farsi)
  27. Malakouti, M. J. & Tehrani, M. M. (1999). Effects of micronutriens on the yield and quality of agricultural products. Tarbiat Modarres University Publications, Tehran, Iran, 292 pp. (in Farsi).
  28. Mahmoodian, M., Mokhtarpour, H. & Kazemi, M. (2002). Various effects of micronutrients on growth, yield and qualitative and quantitative properties of corn. Proceedings of  the 7th Iranian Congrees in Agronomy and Plant Breeding, Seed and Plant Improvement Institute, Karaj, Iran, P, 274. (In Farsi)
  29. Naserirad, H., Soleymanifard, A. & Naseri, R. (2011). Effect of integrated application of biofertilizer on grain yield, yield components and associated traits of maize cultivars. American-Eurasian Journal of Agricultural and Environmental Sciences, 10 (2), 271- 277.
  30. Orchard, T. (1977). Estimating the parameters of plant seedling emergence. Seed Science and Technology, 5, 61-69.
  31. Pahlavan, M. R., Keykha, G. A., Eatesa, G. R., Akbarimoghaddam, H., Kookhan, S. A. & Naroueiral, M. R. (2006). The study of effects Zn, Fe and Mn on quantity and quality of grain wheat. Presented in 18th World Congress of Soil Science, 9-15 July, pp. 23.
  32. Pinto, A., Mota, M. & Varennes, A. (2005). Influence of organic matter on the uptake of zinc, copper and iron by sorghum plants. Science of the Total Environment, 326, 239-247.
  33. Ross, S. M., King, J. R., Donovan, J. T. O. & Spaner, D. (2005). The productivity of oats and berseem clover intercrops. I. Primary growth characteristic and forage quality at four densities of oats. Grass and Forage Science, 60, 74-86.
  34. Reuter, D. J., Alston, A. M. & Farlane, J. D. (1988). Occurrence and correction of manganese deficiency in plant. pp. 205-225. In: Graham et al (Eds.). Manganese in soil and plants. Kluwer Academic Publisher, Dordrecht, The Netherland.
  35. Ramakrishnan, K. & Selvakumar, G. (2012). Effect of biofertilizers on enhancement of growth and yield on Tomato (Lycopersicum esculentum Mill.). International Journal of Research in Botany, 2(4), 20-23.
  36. Sharaf, M.S. (1995(. Response of some medicinal plants to inoculation with a symbiotic N2- fixer. Ph.D. Thesis, Faculty of Agriculture, Ain Shams University, Egypt.
  37. Shiemshi, D. (2007). Leaf chlorosis and stomatal aperture. New Phytologist, 166, 455-461.
  38. Sharafi, S., Tajbakhsh, M., Majidi, M. & Pourmirza, A. (2002). Effect of iron and zinc fertilizer on yield and yield components of two forage corn cultivars in Urmia. Soil and Water, 12, 85-94. (in Farsi)
  39. Scott, J. M., Blair, G. J. & Andrews, A. C. (1997). The mechanics of coating seeds in a small rotating drum. Seed Science and Technology, 25, 281-292.
  40. Scott, D., (1998). Effects of seed coating on establishment. NewZealand Journal of Agricultural Research, 18, 59-67.
  41. Safyan, N., Naderi darbaghshahi, M. R. & Bahari, B. (2012). The effect of microelements spraying on growth, qualitative and quantitative grain corn in Iran. International Research Journal of Applied and Basic Sciences, 3, 2780-2784.
  42. Tandon, H. (1995). Micronutrient in soil, crops and fertilizers. Fertilizer development and consultation organization (C- 110), Greater Kailash, New Delhi, India.
  43. Vessey, J. K. (2003). Plant growth promoting rhizobacteria as biofertilizer. Plant Soil, 255(2): 571-586.
  44. Wilson, D. O., Boswell, F. C., Ohki, K., Parker, M. B., Shuman, L. M. & Jellum, M. D. (1982). Change in soybean seed oil and protein as influenced by manganese nutrition. Crop Science, 22, 948-952.
  45. Yung, G. Y., Chen, Z. H., JIau, C. & Iu, C. Z. (1993). Effects of soil selenium on Se content in rapeseed (B. napus) plants and on their growth and yield. Oil Crops of China. Institute of oil crops, CAB, Wuhan, Hubei, China.
  46. Zhansheng, W., Yao, L., Kaleem, I. & Li, C. (2012). Application efficacy of  biological seed coating agent from‌ combination of PGPR on cotton in the field. Asian International Soil Sciences, 134, 903–910.
  47. Ziaeian, A. (2006). Effect of potassium and zinc application in forage corn cultivation. Iranian Journal of Soil Research(Soil and Water Science), 20 (1), 42-35. (In Farsi)
  48. Zohoryan Mehr, M. (2006). Superabsorbents. Polymer Society Press, 83 pages. (In Farsi)