بررسی الگوی پروتئوم محور جنین گندم (Triticum aestivum) تحت تنش‌خشکی در دو رقم حساس و متحمل به خشکی طی مراحل آغازین جوانه زنی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموختۀ کارشناسی ارشد، گروه زراعت و اصلاح نباتات پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 استاد، گروه زراعت و اصلاح نباتات پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 استادیار، گروه زراعت و اصلاح نباتات پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

4 دانشجوی سابق کارشناسی ارشد، گروه زراعت و اصلاح نباتات پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

چکیده

جوانه­زنی یکی از مهم­ترین مراحل رشدی در گیاهان است که به­شدت از تنش‌های محیطی به­ویژه تنش‌خشکی آسیب می­پذیرد. جوانه­زنی از سه مرحلۀ (فاز) مهم تشکیل شده‌ است که بسیاری از فعالیت­ها پیش از مرحلۀ سوم آن (خروج ریشه­چه) آغاز می­شود. محور جنین بذر نیز با توجه به زنده بودن آن، فعالیت­های بسیاری از نظر سوخت‌وساز (متابولیکی)  به‌ویژه در مرحلۀ اول آن رخ می­دهد که از مهم­ترین این تغییرات می­توان به تغییر در الگوی پروتیین‌گان (پروتئوم) آن اشاره کرد. برای بررسی تغییرات پروتیینی محور جنین گندم (سرداری و قدس به­ترتیب متحمل و حساس) تحت تنش‌خشکی (12- بار) آزمایشی به­صورت فاکتوریل در قالب طرح بلوک کامل تصادفی در سه تکرار بررسی شد. نتایج تحقیق گویای تأثیر منفی تنش‌خشکی بر درصد جوانه­زنی بود و سبب کاهش چشمگیری در آن شد. در تجزیۀ ژل­های الکتروفورزی دوبعدی در محور جنین گندم 1000 لکۀ تکرارپذیر مشاهده شد که تعداد 34 لکه با تفاوت معنی‌دار در سطح 5 درصد گزارش شد. در مقایسۀ رقم متحمل نسبت به حساس از 34 لکه با تفاوت معنی­دار، شمار 22 و 6 لکه در شرایط شاهد، 21 و 10 لکه در شرایط تنش، افزایش و کاهش بیان را نشان دادند. نتایج این آزمایش گویای آن بود که فراوانی لکه­ها به میزان زیادی از زمان، شدت و میزان تنش بستگی دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Proteome analysis of wheat seed embryo (Tritium aestivum) in tolerant and susceptible cultivars under drought stress during early germination phase

نویسندگان [English]

  • Ali Shayanfar 1
  • Reaza Tavakkol Afshari 2
  • Houshang Alizade 3
  • Abolrahman Rasoulnia 4
1 Former Graduate Student, Seed Science and Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj
2 Professor, Seed Science and Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj
3 Assistant Professor, Seed Science and Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj
4 Former Graduate Student, Seed Science and Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj
چکیده [English]

Germination is one the most important growth phases in plants that injury from drought stress extremely. Germination divided to 3 phases although before third phases, many activity (radicle protrusion) initiated. Many metabolically activity occurred specially in first phase since seed embryo is a live organ, although differences in protein patterns arised in this phase. This study was carried out to understand the effect of drought stress on protein patterns in the first phase of germination. In order to analysis the effects of drought stress (-12 bar) on wheat embryo proteome (Sardari and Qods respectively tolerant and susceptiblecultivar), factorial experiment was conducted in a Randomized completely block design with three replications. Drought stress diminished germination percentage and caused adverse effects on the proteins pattern. Two-dimensional gel electrophoresis analysis of wheat embryo axis showed 1000 spots were reproducible that 25 spots between treatments indicated significant differences on 5درصد level. In control condition, in comparison tolerant to susceptiblecultivar, 22 and 6 spots and in stress condition, 21 and 10 spots were down and up regulated respectively. The results indicated that frequency of protein under drought stress depends on severity and amount of stress and imbibition time.
 

کلیدواژه‌ها [English]

  • embryo seed
  • germination
  • Proteomics
  • Wheat
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