نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجو
2 دانشگاه تهران
3 هئیت علمی
4 پردیس کشاورزی و منابع طبیعی کرج، دانشکده علوم زراعی و دامی، گروه زراعت و اصلاح نباتات
5 عضو هئیت علمی
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
To study the effect of miRNA and PGPR on drought tolerance, Sadri and Yas bean genotypes were subjected to drought conditions (FC = 90%, 60% and 30%) and bacterial treatments (no bacteria, isolates B1, B2 and mixture 2 bacterial isolates), using CRD design with three replication using Factorial arrengment, Droght stress was applied five weeks after planting (for 10 days). Second terifoliate and root were used for sampling. The results showed that PGPRs lead to membrane stability, root development, etc. To identify potential miRNAs involved in drought stress the publicly available EST sequences of the plant were obtained from NCBI and blasted against the previously known Plant miRNAs. Ultimately, five distinguished potential miRNAs were acquired in the plant (belonging to four families: miR408, miR5021, miR5565e and miR9559). Candidate miRNAs are involved in growth, stress tolerance, etc. miR9559-3P expression decreased in drought and bacterial inoculation conditions in drought sensitive and tolerant plants. However, in the absence of bacterial inoculation, the expression of miR9559-3P was higher in sensitive plants. In sensitive plants, miR9559-3P may increase sensitivity to drought stress by negatively regulating role and targeting a higher percentage of Target genes. However, the tolerant plant with a special regulatory display and reduced expression of miR9559-3P reduced the degradation of miR9559-3P transcripts, increased the expression of the target gene and improved stress tolerance. Since PGPR modulate miRNA expression and lead to drought tolerance and increase plant yield, so use from these bacteria as biofertilizer can be a suitable alternative method of chemical fertilizer.
کلیدواژهها [English]
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