Co-inoculation with Mesorhizobium ciceri and PGPR can improve chickpea (Cicer arietinum L.) growth in rain-fed and irrigated conditions

نوع مقاله : مقاله پژوهشی


1 Department of Agronomy and Plant Breeding, College of Agriculture, University of Zanjan, Zanjan, Iran

2 Department of Soil Biology, Soil and Water Research Institute, Karaj, Iran


An experiment was conducted to evaluate the effect of co-inoculation with Rhizobium and plant growth promoting rhizobacteria (PGPR) on chickpea growth under rain-fed and irrigated conditions. Treatments included inoculation with PGPR, co-inoculation with two strain of Mesorhizobium ciceri (SWRI3 and SWRI17), co-inoculation with SWRI3+SWRI17, co-inoculation with PGPR+SWRI3+SWRI17 and application of 25 kg nitrogen ha-1 and no fertilizing as a control. Growth components analysis was estimated from growth curves fitted to the relationships between the measured variables and the temperature index measured based on growing degree days. Drought stress due to rainfed condition, caused significant reduction in crop growth parameters; however, bio-fertilizer improved growth significantly. The maximum LAI in the rainfed condition was 0.29 while co-inoculation with PGPR+SWRI3+SWRI17increased it to 0.54. Although, bio-fertilizers increased the time chickpea plants needed to reach their maximum LAI. In the irrigated condition, the highest CGR, TDM and seed yieldirrigated conditionwere 0.42 g m-2 d-1, 32.34g and 2335 kg ha-1 respectively and decreased to 0.23 g m-2 d-1, 18.34g and 1438 kg ha-1 respectively in rain-fed condition that were also obtained from co-inoculation with all bio-fertilizers (PGPR+SWRI3+SWRI17) in both conditions. Overall, results suggest that using bio-fertilizer helps to alleviat drought stress in rainfed condition and could be recommended in semi-arid environment to maximize chickpea yield.


عنوان مقاله [English]

اثر تلقیح باکتری Mesorhizobium ciceri و PGPR بر بهبود رشد نخود (Cicer arietinum L.) در شرایط آبیاری و دیم

نویسندگان [English]

  • وحیده خالق نژاد 1
  • فرهاد جباری 1
  • علیرضا یوسفی 1
  • احمد اصغرزاده 2
1 گروه زراعت و اصلاح نباتات دانشکده کشاورزی دانشگاه زنجان
2 موسسه تحقیقات خاک و آب کرج
چکیده [English]

به‌منظور بررسی اثر تلقیح  نخود با باکتری ریزوبیوم و ریزوباکتری‌های محرک رشد گیاه (PGPR) بر رشد در شرایط آبیاری و دیم آزمایشی به مرحله اجرا درآمد. تیمارهای آزمایش شامل شاهد (عدم کاربرد کود زیستی و شیمیایی)، تلقیح بذر با PGPR، تلقیح بذر با دو نژاد باکتری Mesorhizobium ciceri (SWRI13 و SWRI17)، تلقیح همزمان بذر با کودهای SWRI13+SWRI17، تلقیح همزمان بذر با کودهای PGPR+SWRI3+SWRI17 و مصرف 25 کیلوگرم بر هکتار نیتروژن بود. تجزیه‌وتحلیل مؤلفه‌های رشد بر اساس منحنی رشد برازش داده‌شده با روابط بین پارامترهای اندازه‌گیری شده تخمین زده شد و شاخص دما بر پایه درجه روز رشد (GDD) اندازه‌گیری گردید. تنش خشکی ناشی از شرایط دیم، باعث کاهش معنی‌دار پارامترهای مرتبط با رشد شد؛ درحالی‌که کودهای زیستی به‌صورت معنی‌داری رشد را بهبود بخشیدند. در شرایط دیم حداکثر میزان LAI، 29/0 بود درحالی‌که تلقیح همزمان PGPR+SWRI3+SWRI17، شاخص سطح برگ را به 54/0 افزایش داد. هرچند کودهای زیستی زمان رسیدن گیاه نخود به حداکثر میزان LAI را افزایش دادند.  در شرایط آبیاری، بالاترین میزان CGR، TDM و عملکرد دانه به ترتیب 42/0گرم بر مترمربع در روز، 34/32 گرم و 2335 کیلوگرم بر هکتار بود که این پارامترها در شرایط دیم به ترتیب به 23/0 گرم بر مترمربع در روز، 34/18 گرم و 1438 کیلوگرم بر هکتار کاهش یافت که در هر دو شرایط از تلقیح همزمان با کودهای زیستی (PGPR+SWRI3+SWRI17) به‌دست‌آمده بودند. به‌طورکلی، نتایج نشان داد که کاربرد کودهای زیستی به کاهش تنش خشکی در شرایط دیم کمک می‌کند و می‌تواند در مناطق نیمه‌خشک برای به دست آوردن بالاترین میزان عملکرد نخود توصیه شود.

کلیدواژه‌ها [English]

  • خشکی
  • ریزوبیوم
  • عملکرد دانه
  • کود زیستی
  • وزن خشک کل

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