تاثیر الیسیتورهای غیرزیستی بر آنتی‌اکسیدان‌ها و صفات فیتوشیمیایی مامیران‌کبیر (Chelidonium majus) تحت تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه بین المللی امام خمینی (ره)، دانشکده کشاورزی و منابع طبیعی، گروه تولید و اصلاح نباتات

2 دانشجوی کارشناسی ارشد اصلاح نیاتات، دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران

چکیده

این تحقیق جهت بررسی تاثیر متیل جاسمونات و سالیسیلیک اسید بر ویژگی‌های آنتی‌اکسیدانی و خصوصیات فیزیولوژیکی- بیوشیمیایی گیاه دارویی مامیران کبیر (Chelidonium majus) تحت شرایط تنش خشکی اجرا شد. بدین منظور آزمایشی به صورت فاکتوریل در قالب طرح کاملا تصادفی با چهار تکرار اجرا شد. سه فاکتور مورد مطالعه شامل تنش خشکی در دو سطح (بدون تنش و تنش خشکی به ترتیب با میزان آبیاری 100 و 50 درصد ظرفیت زراعی خاک)، الیسیتور در سه سطح (شاهد؛ صفر، متیل جاسمونات 100 میکرومولار و سالیسیلیک اسید 2 میلی‌مولار) و اندام گیاهی (ریشه، ساقه و برگ) بودند. نتایج حاصل از تجزیه واریانس و مقایسه میانگین‌ها نشان داد که متیل‌جاسمونات و سالیسیلیک‌اسید فعالیت آنزیم کاتالاز (CAT) را کاهش دادند. هر دو الیسیتور سالیسیلیک‌اسید و متیل‌جاسمونات در فعالیت آنزیم سوپراکسید دیسموتاز (SOD) تاثیری نداشتند اما متیل-جاسمونات باعث افزایش فعالیت گایاکول پراکسیداز (GPX) و سالیسیلیک اسید تحت تنش خشکی باعث کاهش فعالیت آسکوربات پراکسیداز (APX) شد. همچنین متیل‌جاسمونات و سالیسیلیک‌اسید باعث کاهش فعالیت APX نسبت به شاهد شدند. متیل‌جاسمونات و سالیسیلیک اسید موجب افزایش کلروفیـل و میزان کاروتنوئیدها، فلاونوئید و آنتوسیانین نسبت به تیمار شاهد شدند. سالیسیلیک اسید در افزایش محتوای کلروفیل و کاروتنوئیدها و متیل جاسمونات در افزایش فلاونوئید و آنتوسیانین نسبت به هم برتر بودند. بنابر نتایج بدست آمده از این پژوهش، متیل‌جاسمونات و سالیسیلیک‌اسید باعث افزایش توانایی گیاه مامیران در پاسخ به تنش خشکی گردیده و علاوه بر اثر محافظتی در برابر اکسیداسیون ناشی از خشکی باعث افزایش رنگیزه‌های فتوسنتزی و برخی متابولیت‌های گیاه شدند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of abiotic elicitors on antioxidants and phytochemical traits of celandine (Chelidonium majus) under drought stress

نویسندگان [English]

  • Sedigheh Fabriki-Ourang 1
  • Haniyeh-Sadat Shahabzadeh 2
1 Department of Plant Breeding, Imam Khomeini International University, Qazvin, Iran
2 M.Sc. Student, Imam Khomeini International University, Qazvin, Iran
چکیده [English]

This experiment was carried out to investigate the effect of methyl jasmonate and salicylic acid on the antioxidant production and physiological-biochemical properties of Chelidonium majus under drought stress condition. For this, an experiment was conducted as factorial based on completely randomized design with four replications in Imam Khomeini International University during 2016-2017. Three factors included drought stress in two levels (non-stress and drought stress with 100% and 50% field capacity irrigation, respectively), elicitor in three levels (control, 100 μm methyl-jasmonate and 2 mM salicylic acid) and plant organs (root, stem and leaf). Analysis of variance and mean comparisons showed that methyl-jasmonate and salicylic acid reduced the activity of CAT enzyme. Both salicylic acid and methyl-jasmonate did not affect the activity of SOD, but methyl-jasmonate increased the activity of GPX and salicylic acid reduced APX activity under drought stress. Also, methyl-jasmonate and salicylic acid reduced APX activity compared to control treatment. The highest amount of protein content was observed in root and then in leaf. Salicylic acid increased total protein content up to 34% compared to control treatment. Both Methyl-jasmonate and salicylic acid increased chlorophyll chlorophyll, carotenoids, flavonoids and anthocyanin compared to control treatment, but salicylic acid was superior in increasing of chlorophyll and carotenoids whereas methyl-jasmonate was superior in increasing of flavonoids and anthocyanin. In conclusion, methyl-jasmonate and salicylic acid have increased the ability of celandine in response to drought stress, and also, in addition to the protective effect against drought-induced oxidation, they increased photosynthetic pigments and some of metabolites.

کلیدواژه‌ها [English]

  • Celandine
  • Drought
  • Methyl jasmonate
  • Salicylic Acid
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