بهبود فتوسنتز، تبادلات گازی و محتوای کلروفیل لوبیا با کاربرد اپی براسینولید در شرایط تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه زنجان

2 دانشیار، دانشگاه زنجان

3 استادیار، دانشگاه زنجان

چکیده

به منظور مطالعه‌ فتوسنتز، تبادلات گازی و محتوای کلروفیل لوبیا با کاربرد براسینواستروئید، پژوهشی در سال زراعی 1395-1394 در مزرعه‌ تحقیقاتی دانشگاه زنجان، به صورت اسپلیت فاکتوریل در قالب طرح بلوک‌های کامل تصادفی در سه تکرار اجرا شد. در این پژوهش، سطوح آبیاری (در دو سطح آبیاری مطلوب و تنش خشکی) در کرت-های اصلی قرار گرفت و ارقام لوبیا (در دو سطح رقم کوشا و ژنوتیپ COS16) و سطوح مختلف براسینواستروئید (در چهار سطح 0، 2، 4 و 6 میکرومولار) به صورت فاکتوریل در کرت‌های فرعی قرار گرفتند. در مرحله‌ گلدهی با قطع آبیاری، تنش خشکی اعمال شد و همزمان با اعمال تنش خشکی، بوته‌های لوبیا، با براسینواستروئید (اپی‌براسینولید) با غلظت‌های ذکر شده محلول‌پاشی شد. نتایج نشان داد که اعمال تنش خشکی بر غلظت CO2 زیر روزنه‌ای، میزان تعرق، هدایت روزنه‌ای، سرعت فتوسنتز و محتوای کلروفیل تأثیر منفی داشت، اما با حذف تنش خشکی و آبیاری مجدد گیاهان بهبود یافتند. کاربرد اپی‌براسینولید باعث به حداقل رساندن تأثیرات منفی تنش خشکی بر فتوسنتز، تبادلات گازی، محتوای کلروفیل و عملکرد دانه لوبیا شد. بالاترین عملکرد دانه با کاربرد غلظت 2 میکرومولار اپی‌براسینولید (با میانگین 2/2068 کیلوگرم بر هکتار) حاصل شد. بنابراین، کاربرد این هورمون را به عنوان راهکاری جهت افزایش مقاومت به تنش خشکی و افزایش عملکرد دانه لوبیا در شرایط آبیاری مطلوب و تنش خشکی می‌توان پیشنهاد نمود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Improvement of photosynthesis, gas exchange and chlorophyll content of bean by application of Epibrassinolide under drought stress condition

نویسندگان [English]

  • mahsa mohammadi 1
  • Afshin Tavakoli 2
  • Majid Pouryousef 2
  • Ehsan Mohsenifard 3
1 University of Zanjan
2 Associate professor, University of Zanjan
3 Assistant Professor, University of Zanjan
چکیده [English]

In order to study the photosynthesis, gas exchange and chlorophyll content of bean by application of Brassinosteroid, an experiment was conducted during 2016-2017 at the research farm of the University of Zanjan, in split factorial base on randomized complete block design with three replications. In this experiment, irrigation levels (in two levels of optimal irrigation and drought stress) were applied to main plots and bean cultivars (at two levels of Kusha cultivar and COS16 genotype) and different levels of Brassinosteroid (at four levels of 0, 2, 4 and 6 μM) were allocated to sub plots as factorial. In the flowering stage, by cutting irrigation, drought stress was applied and simultaneously with drought stress, bean plants, were sprayed with Brassinosteroid (Epibrassinolide) at the indicated concentrations. The results showed that drought stress had negative effects on the intercellular CO2 concentration, transpiration rate, stomatal conductance, photosynthesis rate and chlorophyll content, but with the elimination of drought stress and re-irrigation, the plants were recovered. The use of Epibrassinolide minimized the negative effects of drought stress on photosynthesis, gas exchange, chlorophyll content and seed yield of bean. The highest seed yield was obtained by application of 2 μM of Epibrassinolide (with an average of 2068.2 kg.h-1). Therefore, the use of this hormone can be suggested as a solution to increase drought stress resistance and increase the seed yield of bean under optimal irrigation and drought stress conditions.

کلیدواژه‌ها [English]

  • Intercellular CO2 concentration
  • Photosynthesis rate
  • Seed yield
  • Stomatal conductance
  • transpiration rate
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