عنوان مقاله [English]
In order to study the photosynthesis, gas exchange and chlorophyll content of bean by application of Brassinosteroid, an experiment was conducted during 2016-2017 at the research farm of the University of Zanjan, in split factorial base on randomized complete block design with three replications. In this experiment, irrigation levels (in two levels of optimal irrigation and drought stress) were applied to main plots and bean cultivars (at two levels of Kusha cultivar and COS16 genotype) and different levels of Brassinosteroid (at four levels of 0, 2, 4 and 6 μM) were allocated to sub plots as factorial. In the flowering stage, by cutting irrigation, drought stress was applied and simultaneously with drought stress, bean plants, were sprayed with Brassinosteroid (Epibrassinolide) at the indicated concentrations. The results showed that drought stress had negative effects on the intercellular CO2 concentration, transpiration rate, stomatal conductance, photosynthesis rate and chlorophyll content, but with the elimination of drought stress and re-irrigation, the plants were recovered. The use of Epibrassinolide minimized the negative effects of drought stress on photosynthesis, gas exchange, chlorophyll content and seed yield of bean. The highest seed yield was obtained by application of 2 μM of Epibrassinolide (with an average of 2068.2 kg.h-1). Therefore, the use of this hormone can be suggested as a solution to increase drought stress resistance and increase the seed yield of bean under optimal irrigation and drought stress conditions.
10. Contour-Ansel, D., Torres-Franklin, M.L., Zuily-Fodil, Y. & Cruz de Carvalho, M.H. (2010). An aspartic acid protease from common bean is expressed 'on call' during water stress and early recovery. Journal of Plant Physiology, 167, 1606-1612.
11. Fariduddin, Q., Khanam, S., Hasan, S.A., Ali, B., Hayat, S. & Ahmad, A. (2009). Effect of 28-homobrassinolide on the drought stress induced changes in photosynthesis and antioxidant system of Brassica juncea L. Acta Physiologiae Plantarum, 31, 889-897.
12. Farooq, M., Wahid, A., Kobayashi, N., Fujita, D. & Basra, S.M.A. (2009). Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development, 29, 185-212.
13. Food and Agriculture Organization. (2014). Crops production report from. http://faostat.fao.org
14. German, C., Teran, H. & Allen, R.G. (2006). Selection for drought resistance in dry bean landraces and cultivars. Crop Science, 46, 2111-2120.
15. Gupta, N.K., Gupta, S. & Kumar, A. (2001). Effect of Water Stress on physiological attributes and their relationship with growth and yield of wheat cultivars at different stages. Journal of Agronomy and Crop Science, 186, 55-62.
16. Hosseinzadeh, S.R., Amiri, H. & Ismaili, A. (2015). Effect of vermicompost fertilizer on photosynthetic characteristics of chickpea (Cicer arietinum L.) under drought stress. Photosynthetica, 54(1), 87-92.
17. Keller, J. & Bliesner, R.D. (1990). Sprinkler and trickle irrigation. Anavi book. Van Nostrand Reinhold, New York. Pp 652.
18. Medrano, H., Escalona, J.M., Bota, J., Gulias, J. & Flexas, J. (2002). Regulation of photosynthesis of C3 plant in response to progressive drought: stomatal conductance as a reference parameter. Annals of Botany, 89, 895-905.
19. Miyashita, K., Tanakamaru, S., Maitani, T. & Kimura, K. (2005). Recovery responses of photosynthesis, transpiration, and stomatal conductance in kidney bean following drought stress. Environmental and Experimental Botany, 53(2), 205-214.
20. Munoz-Perea, C.G., Teran, H., Allen, R.G., Wright, J.L., Westermann, D.T. & Singh, S.P. (2006). Selection for drought resistance in dry bean landraces and cultivars. Crop Science, 46, 2111-2120.
21. Reddy, A.R., Chaitanya, K.V. & Vivekanandan, M. (2004). Drought-induced response of photosynthesis and antioxidant metabolism in higher plants. Journal of Plant Physiology, 161, 1189-1202.
22. Sengupta, K., Mitra, S. & Ray, M. (2009). Effect of brassinolide on growth and yield of summer greengram crop. Indian Agriculturist, 53(3/4), 155-157.
23. Szilagyi, L. (2003). Influence of drought on seed yield components in common bean. Bulgarian Journal of Plant Physiology. Special Issue, 320-330.
24. Talaat, N.B. & Shawky, B.T. (2012). 24-Epibrassinolide ameliorates the saline stress and improves the productivity of wheat (Triticum aestivum L.). Environmental and Experimental Botany, 82, 80-88.
25. Talaat, N.B. & Shawky, B.T. (2016). Dual application of 24-epibrassinolide and spermine confers drought stress tolerance in maize (Zea mays L.) by modulating polyamine and protein metabolism. Journal of Plant Growth Regulation. DOI: 10.1007/s00344-015-9557-y.
26. Teran, H. & Singh, S.P. (2002). Comparison of sources and lines selected for drought resistance in common bean. Crop Science, 42(1), 64-70.
27. Thussagunpanit, J., Jutamanee, K., Sonjaroon, W., Kaveeta, L., Chai-Arree, W., Pankean, P. & Suksamrarn, A. (2015). Effects of brassinosteroid and brassinosteroid mimic on photosynthetic efficiency and rice yield under heat stress. Photosynthetica, 53(2), 312-320.
28. Tuba Bicer, B., Narin Kalender, A. & Akar, D.A. (2004). The effect of irrigation on spring-sown Chickpea. Journal of Agronomy, 3, 154-158.
29. Upreti, K.K. & Murti, G.S.R. (2004). Effects of brassinosteroids on growth, nodulation, phytohormone content and nitrogenase activity in French bean under water stress. Biologia Plantarum, 48(3), 407-411.
30. Vardhini, B.V. & Anjum, N.A. (2015). Brassinosteroids make plant life easier under abiotic stresses mainly by modulating major components of antioxidant defense system. Frontiers in Environmental Science. DOI: 10.3389/fenvs.2014.00067.
31. Xia, J.X., Huang, L.F., Zhou, Y.H., Mao, W.H., Shi, K., Wu, J.X., Asami, T., Chen, Z. & Yu, J.Q. (2009). Brassinosteroids promote photosynthesis and growth by enhancing activation of Rubisco and expression of photosynthetic genes in Cucumis sativus. Planta, 230, 1185-1196.
32. Yu, J.Q., Huang, L.F., Hu, W.H., Zhou, Y.H., Mao, W.H., Ye, S.F. & Nogues, S.A. (2004). A role of brassinosteroids in the regulation of photosynthesis in Cucumis sativus. Journal of Experimental Botany, 55, 1135-1143.
33. Zhang, M., Zhai, Z., Tian, X., Duan, L. & Li, Z. (2008). Brassinolide alleviated the adverse effect of water deficits on photosynthesis and the antioxidant of soybean (Glycine max L.). Plant Growth Regulation, 56, 257-264.