ارزیابی ژنوتیپهای گندم نان در شرایط تنش خشکی با استفاده از شاخص‎های تحمل به خشکی و نشانگر های مولکولی SSR

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استاد گروه ژتیک و تولید گیاهی، دانشکده کشاورزی دانشگاه ولی عصر(عج).ایران

2 گروه ژنتیک و تولید گیاهی ، دانشکده کشاورزی، دانشگاه ولی عصر ،ایران

3 گروه ژنتیک و تولید گیاهی ، دانشکده کشاورزی دانشگاه ولی عصر(عج).ایران

4 ژنتیک و تولید گیاهی ، دانشکده کشاورزی، دانشگاه ولی عصر فسنجان, رفسنجان، ایران

چکیده

36 ژنوتیپ گندم نان شامل6 رقم اصلاح شده و30 ژنوتیپ دیگر که از مناطق مختلف ایران و سایر کشورها جمع آوری و تهیه شده اند، در دو محیط رطوبتی (آبیاری نرمال و ‎کم‎آبیاری) در قالب طرح بلوک کامل تصادفی با سه تکرار در هر محیط ،مورد ارزیابی قرار گرفتند و با اندازه گیری میانگین عملکرد دانه در دو محیط برای هر ژنوتیپ، شاخص‎های تحمل و حساسیت به تنش محاسبه گردید و رابطه بین تعدادی از نشانگرهای ریز ماهواره مرتبط با تحمل به خشکی و میزان تحمل به خشکی ژنوتیپ های مورد بررسی مطالعه شد. گروه‎بندی ژنوتیپ‎ها بر اساس شاخص تحمل به تنش (STI)، میانگین هندسی بهره‌وری (GMP)، شاخص میانگین عملکرد (MP) و عملکرد دانه در متر مربع در شرایط تنش (Ys) و شرایط بدون تنش (Yp) با استفاده از تجزیه خوشه‎ای انجام شد و ژنوتیپ‎ها به سه گروه تقسیم شدند. ژنوتیپ‎های 113/11، 1553، 176، 198، 3798، 689، 894، 9016، 910، 99، کوهدشت، پیشتاز، زاگرس، سیوند و ژنوتیپ‎های 1637، سرداری، 2047، 2103، آذر2، 871، 890‎ به‎ترتیب به‏عنوان ژنوتیپ‎های متحمل و نیمه متحمل شناسایی شدند. تجزیه خوشه‎ای بر اساس نشانگر‎های ریز‎ماهواره نیز ژنوتیپ‎ها را به سه گروه تقسیم‎بندی نمود. مقایسه دندروگرام‎ها بیانگر ارتباط معنی‎دار بین تحمل به خشکی و نشانگرهای SSR بود. همچنین تجزیه رگرسیونی بین عملکرد در هر دو شرایط و شاخص‎های GMP وSTI , MP بعنوان متغیرهای وابسته و باندهای نشانگر بعنوان متغیر مستقل نشان داد که آغازگرهای (WMC179، WMC307 و WMC322 ) به عنوان مؤثرترین نشانگر در MAS برای بهبود عملکرد در شرایط تنش و بدون تنش می باشند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of Bread Wheat Genotypes under Drought Stress Conditions using Drought Tolerance indices and SSR Molecular Markers.

نویسندگان [English]

  • hossein dashti 1
  • zohreh dehghan manshadi 2
  • khalil malekzadeh 3
  • Shahab Maddah Hosseini 4
1 department of genetic and plant production,faculty of Agriculture ,vali-asr-university,Rafsanjan Iran
2 department of genetic and plant production,faculty of Agriculture ,vali-asr-university,Rafsanjan Iran
3 department of genetic and plant production,faculty of Agriculture ,vali-asr-university,Rafsanjan Iran
4 department of genetic and plant production,faculty of Agriculture ,vali-asr-university,Rafsanjan Iran
چکیده [English]

36 bread wheat genotypes including 6 improved and introduced cultivars and 30 other genotypes that are collected and prepared from different regions of Iran and other countries were evaluated in two irrigation conditions (normal and limited irrigation) in two separate randomized complete block design with three replications in both moisture conditions. By measuring the average yield for each genotype in each environment, tolerance and stress susceptibility indices were calculated and the relationship between a number of micro satellite markers (associated with drought tolerance) and drought tolerance of genotypes were studied. The genotypes were divided into three groups( tolerant, semi tolerant and susceptible) by cluster analysis based on Stress Tolerance Index (STI), Geometric Productivity Mean (GMP), Mean Performance (MP) and Yield Index (YI) as well as grain yield under stress conditions .
Genotypes 11.113, 1553, 176, 198, 3798, 689, 894, 9016, 910, 99, Kohdasht, Pishtaz, Zagros, Sivand And Genotypes 1637, Sardari, 2047, 2103, Azar 2, 871, 890 were identified as tolerant and semi tolerant genotypes respectively. Cluster analysis based on micro satellite markers also divided the genotypes into three groups. Comparison of dendrograms revealed a significant relationship between drought tolerance and SSR markers. As well as , regression analysis between the grain yield in both conditions, GMP, STI, MP as dependent and marker bands as independent variables showed that(WMC179 , WMC307 and WMC322) are the most effective marker in( MAS) for improving yield under stressed and non-stress conditions.

کلیدواژه‌ها [English]

  • Key words
  • wheat. Drought tolerance
  • index
  • SSR markers
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