بررسی لاین‌های اینبرد نوترکیب برنج از لحاظ تحمل به خشکی با استفاده از شاخص‌های تحمل و نشانگرهای SSR

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه گیلان

2 مرکز تحقیقات کشاورزی و منابع طبیعی

3 دانشگاه یاسوج

چکیده

تنش خشکی همواره یکی از مهم‌ترین عوامل محدودکننده در تولید محصولات گیاهی بوده است. در تحقیق حاضر تعداد 142 لاین اینبرد نوترکیب برنج برنج حاصل از تلاقی ارقام شاه‌پسند و IR28 در بهار و تابستان 1394 در دانشکده کشاورزی دانشگاه گیلان مورد بررسی قرار گرفت. پس از ثبت عملکرد لاین‌ها در شرایط نرمال و تحت تنش خشکی، به منظور شناسایی لاین‌های متحمل به خشکی از شاخص‌های تحمل و حساسیت به تنش و نشانگرهای ریزماهواره پیوسته به خشکی استفاده شد. بر اساس نتایج تجزیه خوشه‌ای، کلیه لاین‌ها به چهارگروه تقسیم شدند. بر اساس این گروه‌بندی دو لاین (37 و 144) به همراه زیر گروه 11 عضوی، در مجموع بر اساس شاخص‌های GMP، STI و MP، به عنوان متحمل‌ترین لاین‌ها شناسایی شدند و میانگین عملکرد 13 لاین مذکور در شرایط بدون تنش و تنش خشکی بترتیب 086/5 و 678/4 تن در هکتار بدست آمد. به منظور اعتبارسنجی در جمعیت حاضر، برای نشانگرهایی که در مطالعات پیشین به عنوان نشانگر پیوسته به QTLهای مرتبط با تحمل به تنش خشکی شناسایی شده بودند، تجزیه رگرسیونی انجام شد. نشانگر RM7 برای همه متغیرهای وابسته، RM231 با عملکرد در هر دو شرایط، STI ، نشانگر RM302 با عملکرد در هر دو شرایط، GMP و MP ارتباط معنی‌دار داشتند و نشانگرهای RM12091، RM19367 و RM10793 در درجه بعدی اهمیت قرار داشتند. تجزیه ارتباط نشان داد 4/51 درصد از تغییرات عملکرد تحت تنش خشکی توسط نشانگرهای RM7، RM231، RM302، RM12091 و RM19367 توجیه شدند که می‌تواند نشاندهنده تایید اهمیت این نشانگرها در زمینه ژنتیکی حاضر باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Investigation of rice recombinant inbred lines based on drought tolerance using tolerance indices and SSR markers

نویسنده [English]

  • Atefeh Sabouri 1
1
2
3
چکیده [English]

Drought stress is considered as one of the most important limiting factors in plant production. In the present study, 142 recombinant inbred lines (RILs) derived from IR28 and Shahpasand were investigated in Faculty of Agricultural Sciences University of Guilan during spring and summer 2015. After recording of yield under normal and drought stress conditions, in order to determine the drought tolerant lines were used tolerance indices and microsatellites markers that identified as linked to drought stress. According to the cluster analysis results, all of lines were divided to four groups. In this grouping two lines (37 and 144) a long with another sub group with 11 lines were most tolerant lines based on tolerance indices (GMP, STI and MP). Average yield of these 13 lines under normal and drought stress condition were 5.086 and 4.678 t/ha respectively. In order to validate identified markers for drought stress identified in previous studies, a stepwise regression was performed. The marker RM7 for all dependents variables, RM231 for yield under two conditions, STI and RM302 for yield under two conditions, GMP, and MP were significant and thereafter RM12091, RM19367 and RM10793 were the next important markers. Association analysis revealed 51.4% of variation of yield under drought stress was explained by RM7, RM231, RM302, RM12091 and RM19367 markers that can be indicative a confirmation of these markers importance in present genetic background.

کلیدواژه‌ها [English]

  • rice
  • Stepwise regression
  • Water deficit
  • microsatellite markers
  • QTL
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