شناسایی نواحی ژنومی کنترل‌کننده صفات فیزیولوژیک و مورفولوژیک گندم نان در شرایط تنش گرمای انتهای فصل

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل

2 دانشیار گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل

3 استاد گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل

4 استادیار گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل

چکیده

چکیده
به‌منظور شناسایی نواحی ژنومی اصلی و اپیستاتیک و اثر متقابل آن‌ها با محیط برای برخی صفات فیزیولوژیک و مورفولوژیک گندم، آزمایشی با 167 لاین خویش آمیخته نوترکیب به همراه والدین آن‌ها (‘‘Babax و ‘SeriM82’) در سال زراعی 95-1394 در مزرعه تحقیقاتی ایستگاه تحقیقات کشاورزی سیستان اجرا گردید. آزمایش در قالب دو طرح آلفا لاتیس با دو تکرار در دو شرایط نرمال و تنش گرمای انتهای فصل اجرا شد‌. صفات روز تا رسیدگی، دوره پر شدن دانه، حداکثر کارایی فتوسیستمII، پایداری غشاء سیتوپلاسمی، تعداد دانه در سنبله، عملکرد دانه و وزن هزار دانه اندازه‌گیری شدند. اثر ژنوتیپ برای کلیه صفات مورد مطالعه معنی‌دار بود و بیشترین میزان همبستگی بین عملکرد دانه و حداکثر کارایی فتوسیستمII (**86/0r =) مشاهده شد. تجزیه QTL به روش ترکیبی بر اساس مکان‌یابی فاصله‌ای مرکب (MCIM) انجام گرفت. برای صفات مورد مطالعه درمجموع سی QTL اصلی مکان‌یابی شد. واریانس فنوتیپی توجیه شده به‌وسیله این QTL ‌ها از 04/7 درصد برای دوره پر شدن دانه تا 41/19 درصد برای وزن هزار دانه متغیر بود. مکان ژنی Qtgw7D درمجاورت نشانگر acc/cat-10 به‌عنوان یک QTL بزرگ اثر (41/19 R2 =) شناسایی شد و پس از تعیین اعتبار می‌تواند در گزینش به کمک نشانگر برای ایجاد ارقام گندم متحمل به گرما مورد استفاده قرار گیرد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Identification of genomic regions controlling physiological and morphological traits of bread wheat under terminal heat stress

نویسندگان [English]

  • Faramarz Sohrabi Chah Hassan 1
  • Barat Ali Fakheri 3
  • Nafiseh Mahdi Nezhad 4
1 Phd.student of Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran.
2 Associate Professor Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran.
3 Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran.
4 Assistant Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran.
چکیده [English]

In order to identify genomic regions with main, epistatic, and QTL×environment interaction effects for some of the phenological and morphological traits in bread wheat, an experiment with 167 recombinant inbred lines and their parents (‘SeriM82’ and ‘Babax’) was conducted at Research Farm of the Agricultural Research Station of Sistan in 2015-16 cropping season. The experiment was carried out using two alpha lattice designs with two replications under non-stress and terminal heat stress conditions. Seven traits including days to maturity (DM), grain filling duration (GFD), maximum quantum efficiency of photosystemII (Fv/Fm), cytoplasmic membrane stability (CMS), grains per spike (GPS), grain yield (GY) and thousand grain weight (TGW) were measured. There were significant differences among the genotypes for all studied traits and maximum correlation was observed between GY and Fm/Fv (r = 0.86**). QTL analysis was conducted by Mixed-Model based composite interval mapping (MCIM) method. A total of 33 main-effect QTLs for studied traits were detected. Phenotypic variances explained by these QTLs varied from 7.04% for GFD to 19.41% for TGW. The major Qtgw7D (R2 = 19/41) was identified near the marker of acc/cat-10 and after validation can be used in marker-assisted selection (MAS) in order to produce heat tolerant wheat varieties.

کلیدواژه‌ها [English]

  • Key words: Epistasis
  • Genomic region
  • Recombinant inbred lines
Ayoub, M., Armstrong, E., Bridger, G., Fortin, M. G. & Mather, D. E. (2003). Marker-based selection in barley for a QTL region affecting alpha amylase activity of malt. Crop Science, 43, 556-561.
Azadi, A., Mardi, M., Hervan, E. M., Mohammadi, S. A., Moradi, F., Tabatabaee, M. T., Pirseyedi, S. M., Ebrahimi, M., Fayaz, F., Kazemi, M., Ashkani, S., Nakhoda, B. & Mohammadi Nejad, G. (2014). QTL mapping of yield and yield components under normal and salt-stress conditions in bread wheat (Triticum aestivum L.). Plant Molecular Biology Reporter, 33, 102-120.
Azam, F., Chang, X. & Jing, R. (2015). Mapping QTL for chlorophyll fluorescence kinetics parameters at seedling stage as indicators of heat tolerance in wheat. Euphytica, 202, 245-258.
Baker, N. R. & Rosengvist, E. (2004). Applications of chlorophyll fluorescence can improve crop production strategies: An examination of future possibilities. Journal of Experimental Botany, 55, 1607-1621.
Barnabas, B., Jager, K. & Feher, A. (2008). The effect of drought and heat stress on reproductive processes in cereals. Plant Cell Environment, 31, 11-38.
Bennett, D., Izanloo, A., Reynolds, M., Kuchel, H., Langridge, P. & Schnurbusch, T. (2012). Genetic dissection of grain yield and physical grain quality in bread wheat (Triticum aestivum L.) under water limited environments. Theoretical Appllied Genetics, 125, 255-271.
Jalal Kamali, M. R. & Duveiller, E. (2008). Wheat Production and Research in Iran: A Success Story. In: International Symposium onWheat Yield Potential: Challenges to International Wheat Breeding. Mexico, D.F. CIMMYT.
Lopes, M. S., Reynolds, M. P., McIntyre, C. L., Mathews, K. L., Jalal Kamali, M. R., Mossad, M., Feltaous, Y., Tahir, I. S. A., Chatrath, R., Ogbonnaya, F. & Baum, M. (2013). QTL for yield and associated traits in the Seri/Babax population grown across several environments in Mexico, in the West Asia, North Africa, and South Asia regions. Theoretical Appllied Genetics, 126, 971-984.
Mason, R. E., Mondal, S., Beecher, F. W., Pacheco, A., Jampala, B., Ibrahim, A. M. & Hays, D. B. (2010). QTL associated with heat susceptibility index in wheat (Triticum aestivum L.) under short-term reproductive stage heat stress. Euphytica, 174, 423-436.
10. McIntyre, C. L., Mathews, K. L., Rattey, A., Chapman, S. C., Drenth, J., Ghaderi, M., Reynolds, M. P. & Shorter, R. (2010). Molecular detection of genomic regions associated with grain yield evaluated under irrigated and rainfed conditions. Theoretical Appllied Genetics, 120, 527-541.
11. Modarresi, M., Mohammadi, V., Zali, A. & Mardi, M. (2010). Response of wheat yield and yield related traits to high temperature. Cereal Research Communications, 38: 23–31.
12. Mohammadi, V., Zali, A. A. & Bihamta, M. R. (2008). Mapping QTLs for heat tolerance in wheat. JournalofAgriculturalScienceandTechnology, 10,261-267.
13. Nyquist, W. E. (1991). Estimation of heritability and prediction of selection response in plant populations. Critical Reviews in Plant Sciences, 10, 235-322.
14. Olivares villegas, J. J., Reynolds, M. P. & McDonald, G. K. (2007). Drought adaptive attributes in the Seri/Babax hexaploid wheat population. Functional Plant Bioliology, 34,189-203.
15. Paliwal, R., Roder, M. S., Kumar, U., Srivastava, J. P. & Joshi, A. K. (2012). QTL mapping of terminal heat tolerance in hexaploid wheat (T. aestivum L.). Theoretical Appllied Genetics, 125,561-575.
16. Panse, V. G. (1957). Genetics of quantitative characters in relation to plant breeding. Indian Journal of Genetics, 17, 317-328.
17. Pinto, R. S., Reynolds, M. P., Mathews, K. L., McIntyre, C. L., Olivares Villegas, J. J. & Chapman, S. C. (2010). Heat and drought adaptive QTL in a wheat population designed to minimize confounding agronomic effects. Theoretical Appllied Genetics, 121, 1001-1021.
18. Rahman, M. A., Chikushi, J., Yoshida, S. & Karim, A. J. M. S. (2009). Growth and yield components of wheat genotypes exposed to high temperature stress under control environment. Bangladesh Journal of Agricultural Research, 34, 361-372.
19. Rane, J. & Nagarajan, S. (2004). High temperature index for field evaluation of heat tolerance in wheat cultivars. Agricultural Systems, 79, 243-255.
20. Reynolds, M. P., Ortiz-Monasterio, J. I. & McNab, A. (2001). Application of Physiology in Wheat Breeding. Mexico, D.F., CIMMYT.
21. Rizza, F., Crossatti, C., Stancan, M. & Cattevelli, L. (1994). Studies for assessing the influences of hardening on cold tolerance of barley genotypes. Euphytica, 75, 131-138.
22. Talukder, S. K., Babar, M. A., Vijayalakshmi, K., Poland, J., Prasad, P. V. V., Bowden, R. & Fritz, A. (2014). Mapping QTL for the traits associated with heat tolerance in wheat (Triticum aestivum L.). MBC Genetics, 1597, 1-13.
23. Teulat, B., Borries, C. & This, D. (2001). New QTLs identified for plant water status, water-soluble carbohydrate and osmotic adjustment in a barley population grown in a growth chamber under two water regimes. Theoretical Appllied Genetics, 103, 161-170.
24. Vijendra Das, L. D. (2000). Problem facing plant breeding. CBS Publishers. 896 p.
25. Wei, L., Bai, S., Li, J., Hou, X., Wang, X., Li, H., Zhang, B., Chen, W., Liu, D., Liu, B. & Zhang, H. (2014). QTL Positioning of Thousand Wheat Grain Weight in Qaidam Basin. Open Journal of Genetics, 4, 239-244.
26. Yang, D. L., Jing, R. L., Chang, X. P. & Li, W. (2007). Quantitative trait loci mapping for chlorophyll fluorescence and associated traits in wheat (Triticum aestivum L.). Journal of Integrative Plant Biology, 49, 646-654.
27. Yang, J., Hu, C., Hu, H., Yu, R., Xia, Z., Ye, X. & Zhu, J. (2008). QTLNetwork: mapping and visualizing genetic architecture of complex traits in experimental populations. Bioinformatics, 24(5), 721-723.