بررسی خصوصیات کاریوتیپ ده جمعیت محوری یونجه(Medicago sativa L.) در ترکیه

نوع مقاله : مقاله پژوهشی

نویسندگان

1 هیات علمی موسسه تحقیقات اصلاح و تهیه نهال و بذر

2 استاد دانشکده کشاورزی گروه اصلاح آنکارا ترکیه

3 استاد دانشکده کشاورزی ایسپارتا ترکیه

چکیده

مطالعات سیتوژنتیکی بر روی ده جمعیت محوری یونجه انتخاب شده از منایع ژرم پلاسم مناطق دریاچه ای استانهای ترکیه برای تولید واریته های سنتتیک انجام گردید. مریستم های نوک ریشه ای حاصله از گیاهان مشابه و هم سن تکثیر یافته از طریق غیرجنسی رویشی از یک والد در گلدانهای گلخانه، در محلول دو هزارم مولار 8- هیدروکسی کینولین پیش تیمار و سپس در رنگ استوایرون هماتوکسیلن رنگ آمیزی شدند. آنالیز کاریوتیپ جمعیت ها نشان داد که همه جمعیت ها تتراپلوئید و دارای 32=x4=n2 کروموزوم هستند. اندازه کروموزومها خیلی کوچک در محدوده 1.69-5.92 میکرون هستند. کروموزومهای B و هترومورف مشاهده شده است. بدین سبب هتروژن بودن جمعیت های یونجه این مناطق محرز شده است. در فرمول کاریوتیپ جمعیت ها که کروموزومهای آنها در 8 تتراد کلاس بندی شده اند دو تیپ کروموزوم (m متاسانتریک و sm ساب متاسانتریک) دیده شده است. تقارن و عدم تقارن کاریوتیپ جمعیت ها با استفاده از شاخص های مختلف؛ استبینس، A1 (درون کروموزومی) و A2 (میان کروموزومی) روش رومرو و زارکو ارزیابی شد. بر اساس تفاوت درون کروموزومی و بین کروموزومی جمعیت ها گروه بندی شدند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Karyotype characterization of ten pivotal populations of Medicago sativa L. in Turkey

نویسندگان [English]

  • Eissa Zarifi 1
  • Cafer Sırrı Sevimay 2
  • Sebahattin Albayrak 3
1 Scientific Board SPII.ir
2 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ankara University, Ankara, Turkey
3 Department of Agronomy, Faculty of Agriculture, Suleyman Demirel University, Isparta, Turkey
چکیده [English]

Cytogenetically studies were carried out on ten selected populations of alfalfa (Medicago sativa L.) of Lake Regions in Turkey. Root tips meristems obtained from plants of similar age that originated by vegetative reproduction from a single parent from plants grown under greenhouse conditions, were pretreated with saturated solution of ά-bromonaphthalene and 0.002 M 8-Hydroxiquinolin before staining with aceto-iron-hematoxylin. Karyotype analysis showed that all populations were tetraploid (2n=4x= 32). The chromosomes are small, ranging from 1.69-5.92 microns in length. B chromosome and heteromorphic chromosomes were established. Due to these phenomenons, the existences of heterogeneous in alfalfa populations were demonstrated from these locations. On the basis of karyotypic formula, the populations had 2 types of chromosomes (m, sm), categorizing them in 8 different classes. Assessment of karyotype symmetry was carried out, using various parameters e.g. Stebbins and A1 (Intrachromosomal) and A2 (Interchromosomal) connected to Romero-Zarco categories. The populations were classified by intrachromosomal and interchromosomal differences.

کلیدواژه‌ها [English]

  • Alfalfa (Medicago sativa L.)
  • Synthetic
  • B chromosome
  • Heteromorphic
  • karyotype
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