نوع مقاله : مقاله پژوهشی
نویسندگان
1 هیات علمی موسسه تحقیقات اصلاح و تهیه نهال و بذر
2 استاد دانشکده کشاورزی گروه اصلاح آنکارا ترکیه
3 استاد دانشکده کشاورزی ایسپارتا ترکیه
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Cytogenetically studies were carried out on ten selected populations of alfalfa (Medicago sativa L.) of Lake Regions in Turkey. Root tips meristems obtained from plants of similar age that originated by vegetative reproduction from a single parent from plants grown under greenhouse conditions, were pretreated with saturated solution of ά-bromonaphthalene and 0.002 M 8-Hydroxiquinolin before staining with aceto-iron-hematoxylin. Karyotype analysis showed that all populations were tetraploid (2n=4x= 32). The chromosomes are small, ranging from 1.69-5.92 microns in length. B chromosome and heteromorphic chromosomes were established. Due to these phenomenons, the existences of heterogeneous in alfalfa populations were demonstrated from these locations. On the basis of karyotypic formula, the populations had 2 types of chromosomes (m, sm), categorizing them in 8 different classes. Assessment of karyotype symmetry was carried out, using various parameters e.g. Stebbins and A1 (Intrachromosomal) and A2 (Interchromosomal) connected to Romero-Zarco categories. The populations were classified by intrachromosomal and interchromosomal differences.
کلیدواژهها [English]
10. Bauchan, G. R. & Hossain, M. A. (1998b). Cytogenetic studies of the nine germplasm sources of alfalfa. In Proc. North Am. Alfalfa Improvement Conf., 36th, Bozeman, MT (pp. 2–6).
11. Bauchan, G. R. & Hossain, M. A. (1999a). Constitutive heterochromatin DNA polymorphisms in diploid Medicago sativa ssp. falcata. Genome, 42(5), 930–935.
12. Bauchan, G. R. & Hossain, M. A. (1999b). Detection of chromosome variations in Medicago sativa. In Eucarpia Medicago Symposium Proceedings.
13. Bauchan, G. R. & Hossain, M. A. (2001a). A computerized image analysis system to characterize small plant chromosomes. Microscopy and Analysis, 9–12.
14. Bauchan, G. R. & Hossain, M. A. (2001b). Distribution and characterization of heterochromatic DNA in the tetraploid African population alfalfa genome. Crop Science, 41(6), 1921–1926.
15. Bingham, E. T. (1968). Transfer of Diploid Medicago spp. Germplasm to Tetraploid M.sativa L. in 4x-2x Crosses. Crop Science, 8(6), 760–762.
16. Bingham, E. T., & McCoy, T. J. (1988). Cytology and Cytogenetics of Alfalfa. In Alfalfa and Alfalfa Improvement (pp. 737–776).
17. Bingham, E. T. & Saunders, J. W. (1974). Chromosome Manipulations in Alfalfa: Scaling the Cultivated Tetraploid to Seven Ploidy Levels1. Crop Science, 14(3), 474–477.
18. Brummer, E. C., Cazcarro, P. M. & Luth, D. (1999). Ploidy determination of alfalfa germplasm accessions using flow cytometry. Crop Science, 39(4), 1202–1207.
19. Camacho, J. P. M., Sharbel, T. F. & Beukeboom, L. W. (2000). B-chromosome evolution. Philosophical Transactions of the Royal Society B: Biological Sciences, 355(1394), 163–178.
20. Falistocco, E., Falcinelli, M. & Veronesi, F. (1995). Karyotype and C‐banding pattern of mitotic chromosomes in alfalfa, Medicago sativa L. Plant Breeding, 114(5), 451–453.
21. Frame, J., Charlton, J. F. L. & Laidlaw, A. S. (1998). Temperate forage legumes. Wallingford: CAB INTERNATIONAL.
22. Gillies, C. B. (1970). Alfalfa Chromosomes. I. Pachytene Karyotype of a Diploid Medicago falcata L. and Its Relationship to M.sativa L.1. Crop Science, 10(2), 169–171.
23. Gillies, C. B. (2009). Pachytene chromosomes of perennial Medicago species. Hereditas, 72(2), 277–288.
24. Güleç, T. E. (2010). Bitkilerde Markör Destekli Seleksiyon. Türk Bilimsel Derlemeler Dergisi, 3(2), 67–79.
25. Hanson, A. A., Barnes, D. K., Hill, R. R. & Bauchan, G. R. (1988). The Genus and the Origin of the Comp. In Alfalfa and alfalfa improvement (pp. 93–124).
26. Havananda, T., Brummer, E. C. & Doyle, J. J. (2011). Complex patterns of autopolyploid evolution in alfalfa and allies (Medicago sativa; Leguminosae). American Journal of Botany, 98(10), 1633–1646.
27. Havananda, T., Brummer, E. C., Maureira-Butler, I. J. & Doyle, J. J. (2010). Relationships among Diploid Members of the Medicago sativa (Fabaceae) Species Complex Based on Chloroplast and Mitochondrial DNA Sequences. Systematic Botany, 35(1), 140–150.
28. Hossain, M. A. & Bauchan, G. R. (1999). Brief communication. Identification of B chromosomes using Giemsa banding in Medicago. Journal of Heredity, 90(3), 428–429.
29. Huziwara, Y. (1962). Karyotype Analysis in Some Genera of Compositae. VIII. Further Studies on the Chromosomes of Aster. American Journal of Botany, 49(2), 116.
30. Katepa-Mupondwa, F. M., Christie, B. R. & Michaels, T. E. (2002). An improved breeding strategy for autotetraploid Alfalfa (Medicago sativa L.). Euphytica, 123(1), 139–146.
31. Kingston-Smith, A. H., Marshall, A. H. & Moorby, J. M. (2012). Breeding for genetic improvement of forage plants in relation to increasing animal production with reduced environmental footprint. Animal, 7(s1), 1–10.
32. Lavania, U. C. & Srivastava, S. (1992). A simple parameter of dispersion index that serves as an adjunct to karyotype asymmetry. Journal of Biosciences, 17(2), 179–182.
33. Lavania, U. C. & Srivastava, S. (1999). Quantitative delineation of karyotype variation in Papaver as a measure of phylogenetic differentiation and origin. Current Science, 77(3), 429–435.
34. Lesins, K. A. & Lesins, I. (1979). Genus Medicago (Leguminosae). A taxogenetic study. Dr. W. Junk Publishers, The Hague. Dordrecht: Springer Netherlands.
35. Lesins, K. & Gillies, C. B. (1972). Taxonomy and Cytogenetics of Medicago. In Alfalfa Science and Technology (Vol. agronomymo, pp. 53–86). American Society of Agronomy.
36. LEVAN, A., FREDGA, K. & SANDBERG, A. A. (1964). Nomenclature for Centromeric Position on Chromosomes. Hereditas, 52(2), 201–220.
37. Masoud, S. A., Gill, B. S. & Johnson, L. B. (1991). C-Banding of Alfalfa Chromosomes: Standard Karyotype and Analysis of a Somaclonal Variant. Journal of Heredity, 82(4), 335–338.
38. McCoy, T. J. & Bingham, E. T. (1988). Cytology and Cytogenetics of Alfalfa. In Alfalfa and Alfalfa Improvement (pp. 737–776).
39. McCoy, T. J. & Bingham, E. T. (1991). Alfalfa cytogenetics. Chromosome Engineering in Plants: Genetics, Breeding, Evolution. Part B. Edited by T. Tsuchiya and PK Gupta. Elsevier, Amsterdam, The Netherlands, 399–418.
40. McKersie, B. D. & Bowley, S. R. (1993). Synthetic seeds of alfalfa. Synseeds: Applications of Synthetic Seeds to Crop Improvement, 231–255.
41. Michaud, R., Lehman, W. F. & Rumbaugh, M. D. (1988). World distribution and historical development. Alfalfa and Alfalfa Improvement, 29(29), 25–56.
42. Mujeeb-Kazi, A., Roldan, S., Suh, D. Y., Sitch, L. A. & Farooq, S. (1987). Production and cytogenetic analysis of hybrids between Triticum aestivum and some caespitose Agropyron species. Genome, 29(4), 537–553.
43. Pfeiffer, T. W. & Bingham, E. T. (1983). Abnormal meiosis in alfalfa, Medicago sativa : cytology of 2 N egg and 4 N pollen formation. Canadian Journal of Genetics and Cytology, 25(2), 107–112.
44. Poehlman, J. M. (1987). Breeding Field Crops. Dordrecht: Springer Netherlands.
45. Quiros, C. F. & Bauchan, G. R. (1988). The genus Medicago and the origin of the Medicago sativa complex. Alfalfa and Alfalfa Improvement, 29, 93–124.
46. Quiros, C. F. & Morgan, K. (1981). Peroxidase and leucine-aminopeptidase in diploidMedicago species closely related to alfalfa: Multiple gene loci, multiple allelism, and linkage. Theoretical and Applied Genetics, 60(4), 221–228.
47. Reeves, A. (2001). MicroMeasure: A new computer program for the collection and analysis of cytogenetic data. Genome, 44(3), 439–443.
48. Rieger, R., Michaelis, A. & Green, M. M. (1991). Glossary of Genetics. Berlin, Heidelberg: Springer Berlin Heidelberg.
49. Romero Zarco, C. (1986). A new nethod for estimating karyotype asymmetry. Taxon, 35(3), 526–530.
50. Russelle, M. (2001). Alfalfa After an 8,000-year journey, the “Queen of Forages” stands poised to enjoy renewed popularity. American Scientist, 89(3), 252–261.
51. Şakiroǧlu, M., Doyle, J. J. & Brummer, E. C. (2010). Inferring population structure and genetic diversity of broad range of wild diploid alfalfa (Medicago sativa L.) accessions using SSR markers. Theoretical and Applied Genetics, 121(3), 403–415.
52. Schlarbaum, S. E., Johnson, L. B. & Stuteville, D. L. (1988). Characterization of somatic chromosome morphology in alfalfa, Medicago sativa L.: Comparison of donor plant with regenerated protoclone. CYTOLOGIA, 53(3), 499–507.
53. Small, E. & Bauchan, G. R. (1984). Chromosome numbers of the Medicago sativa complex in Turkey. Canadian Journal of Botany, 62(4), 749–752.
54. Small, E. & Jomphe, M. (1989). A synopsis of the genus Medicago (Leguminosae). Canadian Journal of Botany, 67(11), 3260–3294.
55. Stebbins, G. L. (1971). Chromosomal evolution in higher plants. London: Edward Arnold Ltd.,.
56. Tuğay, E. (1997). Genel Bitki ıslahı. GOP Üniv. Ziraat Fak. Tokat, Turkey.
57. Veronesi, F., Brummer, E. C., & Huyghe, C. (2010). Alfalfa. In B. Boller, U. K. Posselt, & F. Veronesi (Eds.), Fodder Crops and Amenity Grasses (pp. 395–437). New York, NY: Springer New York.
58. Watanabe, K., Yahara, T., Denda, T. & Kosuge, K. (1999). Chromosomal Evolution in the Genus Brachyscome (Asteraceae, Astereae): Statistical Tests Regarding Correlation Between Changes in Karyotype and Habit Using Phylogenetic Information. Journal of Plant Research, 112(2), 145–161.
59. Yu, F., Lei, Y., Li, Y., Dou, Q., Wang, H. & Chen, Z. (2013). Cloning and characterization of chromosomal markers in alfalfa (Medicago sativa L.). Theoretical and Applied Genetics, 126(7), 1885–1896.
60. Zarifi, E., Agayev, Y. ., Ganavati, F. & Aminizadeh, Z. (2005). Cytogenetics and evolution of Karyotype in wormwood, Artemisia vulgaris L. Seed and Plant, Journal of Agricultural Research, 22(1), 1–12.
61. Zarifi, E. & Güloğlu, D. (2016). An improved Aceto-Iron-Haematoxylin staining for mitotic chromosomes in Cornelian cherry ( Cornus mas L.). Caryologia, 7114(January), 1–6.
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