تجزیه و تحلیل مقاومت به بیماری بلایت فوزاریومی سنبله در ژنوتیپهای گندم امیدبخش سال 1392 اقلیم گرم و مرطوب شمال

نوع مقاله : مقاله پژوهشی

نویسندگان

1 مسئول واحد پاتولوژی بخش تحقیقات غلات/مؤسسه تحقیقات اصلاح و تهیه نهال و بذر

2 مربی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان گلستان، سازمان تحقیقات، آموزش و ترویج کشاورزی، گرگان، ایران

3 مربی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان اردبیل، سازمان تحقیقات، آموزش و ترویج کشاورزی، پارس آباد، ایران

چکیده

بیماری بلایت فوزاریومی سنبله که توسط گونه های مختلف قارچ Fusarium به ویژه گونه Fusarium graminearum ایجاد میشود یکی از مهمترین بیماریهای گندم در ایران مخصوصاً در اقلیم گرم و مرطوب کشور است که باعث کاهش عملکرد و کیفیت محصول میشود. در این پروژه تعداد 20 ژنوتیپ گندم مربوط به آزمایشات مقایسه عملکرد امیدبخش اقلیم گرم و مرطوب شمال کشور در سال 1392 (ERWYT-N-1392) طی دو سال در شرایط مزرعه در دو منطقه گرگان و مغان نسبت به بیماری بلایت فوزاریومی سنبله با هدف شناسایی و گزینش ژنوتیپهای دارای مقاومت به بیماری، مورد آزمایش قرار گرفتند. ارزیابی ژنوتیپهای آزمایشی و تجزیه مرکب داده های مربوط به میزان وقوع، شدت و شاخص بیماری و میزان آلودگی دانه به بیماری نشان داد که برای هر چهار صفت گفته شده، تفاوت معنی داری بین ژنوتیپها وجود دارد. نتایج این تحقیق همچنین نشان داد که پنج ژنوتیپ آزمایشی N-92-3، N-92-1 (رقم مروارید)، N-92-19، N-92-4 و N-92-9 با داشتن پایینترین میانگین وقوع، شدت و شاخص بیماری و آلودگی دانه، و کسب بهترین میانگین رتبه از چهار صفت مورد بررسی، به عنوان مقاومترین ژنوتیپهای آزمایشی به شمار میروند. پایین بودن بودن میزان وقوع بیماری و آلودگی دانه در ژنوتیپهای گفته شده میتواند دلیلی بر وجود به ترتیب مقاومت نوع I و IV در آنها باشد. براساس نتایج به دست آمده از تحقیق حاضر، چهار ژنوتیپ آزمایشی N-92-3، N-92-19، N-92-4 و N-92-9 در صورت داشتن عملکرد بالا و مقاومت مطلوب در برابر سایر بیماریها میتوانند کاندیدهای مناسبی برای جایگزینی ارقام قدیمیتر در این اقلیم باشند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Analysis of Resistance to Fusarium Head Blight (FHB) among the Genotypes of 2013 Elite Wheat Genotypes of the North Warm and Humid Zone in Iran

نویسندگان [English]

  • Ali Malihipour 1
  • Mohammadali Dehghan 2
  • Kamal Shahbazi 3
1 Head of Cereal Pathology, Cereal Research Department, Seed & Plant Improvement Institute (SPII)
2 Instructor, Golestan Agricultural and Natural Resources Research and Education Center, AREEO, Gorgan, Iran
3 Instructor, Ardabil Agricultural and Natural Resources Research and Education Center, AREEO, Parsabad, Iran
چکیده [English]

Fusarium head blight (FHB), which is caused mainly by Fusarium graminearum, is one of the most important diseases of wheat in Iran which reduces wheat yield and quality especially in northern parts of the country. For the purpose of identifying and selecting FHB-resistant genotypes, the reactions of 20 lines of the Elite Regional Wheat Yield Trials from the North warm and humid zone in 1392 (ERWYT-N-1392) were determined to FHB under field conditions in two locations for two years. Results of combined analysis of variance of several datasets including disease incidence, severity, and index and Fusarium-damaged kernels (FDK) from two locations (Gorgan and Moghan) in two years (1393 and 1394) showed significant differences among the genotypes for all datasets. Results of the present investigation showed that five genotypes of N-92-3, N-92-1 (cv. Morvarid), N-92-19, N-92-4, and N-92-9 having the lowest disease incidence, severity and disease index and FDK, and the best means of ranks from these traits were the most resistant lines under field conditions. Low disease incidence and FDK in these genotypes may indicate the presence of type I and type IV resistances, respectively. Based on the results of this research, the experimental lines N-92-3, N-92-19, N-92-4, and N-92-9 may be proposed as candidate lines to replace the older cultivars in this region, if their high yeild and resistance to other diseases is confirmed as well.

کلیدواژه‌ها [English]

  • Wheat
  • Fusarium head blight (FHB)
  • Fusarium graminearum
  • Resistance
  • North warm and humid zone
  1. 1.  Arthur, J.C. (1891). Wheat scab. Indiana Agricultural Experiment Station Bulletin 36: 129-138.

2. Bai, G.H. & Shaner, G.E. (1994). Scab of wheat: prospects for control. Plant Disease 78: 760-766.

3. Bai, G.-H. & Shaner, G.E. (2004). Management and resistance in wheat and barley to Fusarium head blight. Annual Review of Phytopathology 42: 135-161.

4. Bai, G.-H., Kolb, F. L., Shaner, G. & Domier, L. L. (1999). Amplified fragment length polymorphism markers linked to a major quantitative trait locus controlling scab resistance in wheat. Phytopathology 89:343-348.

5. Bamdadian, A. & Torabi, M. (1983). Important Diseases of Wheat and Barely and Methods of Scoring. Plant Pests and Diseases Research Institute, Tehran, Iran. 67 pp. (In Farsi).

6. Ban, T. (1997). Evaluation of resistance to Fusarium head blight in indigenous Japanesespeciesof Agropyron (Elymus). Euphytica 97: 39-44.

7. Bolanos-Carriel, C., Wegulo, S.N., Hallen-Adams, H., Baenziger, P.S., Eskridge, K.M. & Funnell-Harris, D. (2016). Effects of cultivar resistance, fungicide application timing, and fungicide chemical class on FHB and DON in winter wheat. In: S. Canty,  A. Clark, K. Wolfe, and D. Van Sanford (Eds.), Proceedings of the 2016 National Fusarium Head Blight Forum ( pp. 9-10). East Lansing, MI/Lexington, KY, USA.

8. Buerstmayr, H., Ban, T. & Anderson, J.A. (2009). QTL mapping and marker-assisted selection for Fusarium head blight resistance in wheat: a review. Plant Breeding 128: 1-26.

9. Buerstmayr, H., Stierschneider, M., Steiner, B., Lemmens, M., Griesser, M., Nevo, E. & Fahima, T. (2003). Variation for resistance to head blight caused by Fusarium graminearum in wild emmer (Triticum dicoccoides) originating from Israel. Euphytica 130: 17-23.

10. Chen, P. (2016). Improvement  of wheat Fusarium head blight (FHB) resistance in China. In: E.M. Del Ponte,  G.C. Bergstrom, W. Pavan, A. Lazzaretti, and J.M.C. Fernandes (Eds.), Proceedings of the 5th International Symposium on Fusarium Head Blight ( p. 18). Universidade de Passo Fundo, Passo Fundo, RS, Brazil.

11. Chen, P.D., & Liu, D.J. (2000). Transfer of scab resistance genes from Leymous racemosus, Roegneria ciliaris, and Roegneria kamoji into commn wheat. In: W.J. Raupp, Z. Ma, P.D. Chen, and D.J. Liu (Eds.), Proceedings of the International Symposium on Wheat Improvement for Scab Resistance, (pp. 62-67). Suzhou and Nanjing, China.

12. Chen, Q., Eudes, F., Conner, R.L., Graf, R., Comeau, A., Collin, J., Ahmad, F., Zhou, R., Li, H., Zhao, Y. & Laroche, A. (2001). Molecular cytogenetic analysis of a durum wheat x Thinopyrum distichum hybrid used as a new source of resistance to Fusarium head blight in the greenhouse. Plant Breeding 120: 375-380.

13. Etebarian, H.R., & Torabi, M. (1996). Studies on resistance of wheat cultivars to fusarium head blight. Iranian Journal of Plant Pathology 32: 9-21. (In Farsi).

14. Fedak, G., Han, F., Cao, W., Burvill, M., Kritenko, S. & Wang, L. (2003). Identification and characterization of novel sources of resistance to FHB. In: N.E. Pogna and R.A. McIntosh (Eds.), Proceedings of 10th International Wheat Genetics Symposium, (pp. 354-356). Paestum, Italy.

15. Foroutan, A., Nategh, Z., Olady, M. & Rostami, F. (1995). Selection tolerant materials of wheat to scab in Mazandaran. In: Proceedings of 12th Iranian Plant Protection Congress, (p. 49). Karaj, Iran.

16. Gervais, L., Dedryver, F., Morlais, J.-Y., Bodusseau, V., Negre, S., Bilous, M., Groos, C. & Trottet, M. (2003). Mapping of quantitative trait loci for field resistance to Fusarium head blight in an European winter wheat. Theoretical and Applied Genetics 106: 961-970.

17. Gilbert, J. & Haber, S. (2013). Overview of some recent research developments in fusarium head blight of wheat. Canadian Journal of Plant Pathology 35: 149-174.

18. Golzar, H. (1995). Record of sources of resistance to Fusarium head blight. In: Proceedings of 12th Iranian Plant Protection Congress, (p. 51). Karaj, Iran.

19. Han, F. & Fedak, G. (2003). Molecular characterization of partial amphiploids from Triticum durum x tetraploid Thinopyrum elongatum as novel sources of resistance to wheat Fusarium head blight. In: N.E. Pogna and R.A. McIntosh (Eds.), Proceedings of 10th International Wheat Genetics Symposium, (pp. 1148-1150). Paestum, Italy.

20. Ketata H., Yan S.K. & Nachit M. (1989). Relative consistency performance across environments. Int. Symposium on Physiology and Breeding of Winter Cereals for stressed Mediterranean Environments. Montpellier, July 3–6, 1989.

21. Liu, W.X., Chen, P.D. & Liu, D.J. (2000). Radiation-induced Triticum aestivum-Leymous racemosus translocations and their molecular cytogenetic analysis. In: W.J. Raupp, Z. Ma, P.D. Chen, and D.J. Liu (Eds.), Proceedings of the International Symposium on Wheat Improvement for Scab Resistance, (pp. 73-76). Suzhou and Nanjing, China.

22. Lu, W.Z., Luo, M. & Shen, T.M. (2016). Breeding and improvement of wheat scab resistance in Yellow and Huai Valleys winter wheat zone, China. In: E.M. Del Ponte,  G.C. Bergstrom, W. Pavan, A. Lazzaretti, and J.M.C. Fernandes (Eds.), Proceedings of the 5th International Symposium on Fusarium Head Blight ( p. 35). Universidade de Passo Fundo, Passo Fundo, RS, Brazil.

23. Malihipour, A., Saidi, A., Alizadeh, A., Dehghan, M.A. & Foroutan, A.R. (2000). Evaluation of resistance of some spring wheat advanced lines and cultivars to Fusarium head blight in Iran. In: Z. Bedo and L. Láng (Eds.), Proceedings of 6th International Wheat Conference, (p. 184). Budapest, Hungary.

24. McKendry, A.L., Tague, D.N., Wright, R.L., Tremain, J.A. & Conley, S.P. (2005). Registration of ‘Truman’ wheat. Crop Science 45: 421-423.

25. McMullen, M., Jones, R. & Gallenberg, D. (1997). Scab of wheat and barley: A reemerging disease of devastating impact. Plant Disease 81: 1340-1348.

26. Mergoum, M., Frohberg, R.C., Miller, J.D. & Stack, R.W. (2005). Registration of ‘Steele-ND’ wheat. Crop Science 45: 1163-1164.

27. Mergoum, M., Frohberg, R.C., Miller, J.D., Stack, R.W., Olsona, T., Friesen, T.L. & Rasmussen, J.B. (2006). Registration of ‘Glenn’ wheat. Crop Science 46: 473-474.

28. Mesterházy, A. (1995). Types and components of resistance to fusarium head blight of wheat. Plant Breeding 114: 377-386.

29. Miller, J.D., Young, J.C. & Sampson, D.R. (1985). Deoxynivalenol and fusarium head blight resistance in spring cereals. Journal Phytopathology 113: 359-367.

30. Parry, D.W., Jenkinson, P. & McLeod, L. (1995). Fusarium ear blight (scab) in small grains-a review. Plant Pathology 44: 207-238.

31. Ruckenbauer, P., Buerstmayr, H. & Lemmens, M. (2001). Present strategies in resistance breeding against scab (Fusarium spp.). Euphytica 119: 121-127.

32. Rudd, J.C., Horsley, R.D., McKendry, A.L. & Elias, E.M. (2001). Host plant resistance genes for fusarium head blight: Sources, mechanisms, and utility in conventional breeding systems. Crop Science 41: 620-627.

33. Schroeder, H.W., & Christensen, J.J. (1963). Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology 53: 831-838.

34. Shen, X., Kong, L. & Ohm, H.W. (2004). Fusarium head blight resistance in hexaploid wheat (Triticum aestivum)-Lophopyrum genetic lines and tagging of the alien chromatin by PCR markers. Theoretical and Applied Genetics 108: 808-813.

35. Snijders, C.H.A. (1990). Genetic variation for resistance to Fusarium head blight in bread wheat. Euphytica 50: 171-179.

36. Stack, R. W. & McMullen, M. P. (1994). A visual scale to estimate severity of Fusarium head blight in wheat-PP1095. North Dakota State University, Fargo, ND, USA.

37. Sutton, J. (1982). Epidemiology of wheat head blight and maize ear rot caused by Fusarium graminearum. Canadian Journal of Plant Pathology 4: 195-209.

38. Waldron, B.L., Moreno-Sevilla, B., Anderson, J.A., Stack, R.W. & Frohberg, R.C. (1999). RFLP mapping of QTL for fusarium head blight resistance in wheat. Crop Science 39: 805-811.

39. Wan, Y.-F., Yen, C. & Yang, J.-L. (1997a). The diversity of head-scab resistance in Triticeae and their relation to ecological conditions. Euphytica 97: 277-281.

40. Wan, Y.-F., Yen, C., Yang, J.-L. & Liu, F.-Q. (1997b). Evaluation of Roegneria for resistance to head scab caused by Fusarium graminearum Schwabe. Genetic Resources and Crop Evolution 44: 211-215.

41. Wang, Y.-Z. & Miller, J.D. (1988). Effects of Fusarium graminearum metabolites on wheat tissue in relation to Fusarium head blight resistance. Journal of Phytopathology 122: 118-125.

42. Wegener, M. (1992). Optimierung von saatgutpillierungen mit mikrobiellen antagonisten zur biologischen biologischen bekampfung von Fusarium culmorum (W. G. Smith) Sacc. in weizen. Diplomarbeit, Georg-August-Universitaet Göttingen, Göttingen, Germany.

43. Wood, M., Comis, D., Harden, D., McGraw, L. & Stelljes, K. B. (1999). Fighting Fusarium. Agricultural Research. June issue. United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, USA.

44. Yang, Z., Gilbert, J., Fedak, G. & Somers, D.J. (2005). Genetic characterization of QTL associated with resistance to Fusarium head blight in a doubled-haploid spring wheat population. Genome 48: 187-196.