مطالعه فعالیت اینورتازها و غلظت کربوهیدرات‌های محلول در مراحل نموی چند گونه گل‌جالیز در گوجه‌فرنگی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشکده علوم و مهندسی کشاورزی-پردیس کشاورزی و منابع طبیعی-دانشگاه تهران

2 استاد گروه زراعت و اصلاح نباتات دانشگاه تهران

چکیده

گل جالیز یک تمام‌انگل اجباری در بسیاری از گیاهان زراعی مهم ازجمله گوجه‌فرنگی است. موفقیت در کنترل گل جالیز ناچیز بوده، بنابراین شناخت سازوکار مصرف ساکارز به‌ عنوان یک فرآیند حیاتی برای انگل می‌تواند به توسعه استراتژی‌های کنترلی اختصاصی کمک کند. این انگل به‌عنوان سینک رقابتی برای ساکارز بوده، آن را در غده تجزیه و به گلوکز و فروکتوز تبدیل می‌کند. اینورتازها مهم‌ترین آنزیم‌های تجزیه‌کننده ساکارز و تنظیم اسمزی در انگل می‌باشند. در این مقاله، فعالیت دو اینورتاز (PrSAI‌‍‍ و PrCWI) درگیر در تبدیل ساکارز در مراحل مختلف نموی سه گونه انگل (مصری، راموزا و نانا) هنگام اتصال به گوجه‌فرنگی بررسی شد. مشاهدات مؤید فعالیت این دو آنزیم در همه مراحل رشدی گونه‌های مختلف انگل است. اما میزان فعالیت آنزیم PrSAI‌‍‍ همواره بالاتر از PrCWI‍ بود. باگذشت زمان، میزان فعالیت PrSAI افزایش ولی میزان فعالیت PrCWI کاهش یافت. فعالیت آنزیم‌ها، میزان پروتئین و قند در گونه مصری در مراحل اولیه بالاتر بوده و با ظهور سریع‌تر این گونه در میزبان ارتباط داشت ولی این فعالیت در گونه راموزا در مراحل انتهایی بیشتر بوده که با تعداد شاخه و بیوماس بالاتر مطابقت داشت. نتایج نشان داد که ژن‌های کد کننده PrSAI و احتمالاً PrCWI می‌توانند اهداف خوبی برای استراتژ‌ی خاموشی ژن در جهت کنترل انگل باشند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Study of invertases activity and soluble sugar concentration at developmental stages of some broomrape species on tomato

نویسندگان [English]

  • Zahra Farrokhi 1
  • Hassan Alizadeh 2
  • Houshang Alizadeh 1
1 Faculty of Agricultural Sciences and Engineering-College of Agriculture and Natural resources-University of Tehran
2 University of Tehran
چکیده [English]

Broomrape is an obligate parasite in many economically important crops such as tomato. Success in controlling broomrape is rare hence an understanding of sucrose utilization mechanisms as a critical process for parasite provides developing specific control strategies. This parasite acts as a competitive sink for sucrose, utilizes it in the tuber and converts it to glucose and fructose. Invertases are the major enzymes in sucrose degradation and osmotic adjustment in the parasite. In this paper, the activity of two invertases (PrSAI and PrCWI) involved in different developmental stages of some broomrape species (Phelipanche aegyptiaca, P. ramosa and P. nana) on tomato was studied. Results showed that activity of both mentioned enzymes were detected in all of the developmental stages in the broomrape species; although the PrSAI activity was always higher than the PrCWI activity. The activities of PrSAI and PrCWI was increased and decreased, respectively, as progressing of the developmental stages of broomrape. Enzymes activity and protein and sugar amounts in Egyptian broomrape were high at the early stages and in branched broomrape in the late stages, the first case accorded to the earlier emergence of broomrape on the host and the later one accorded to more shoots and higher biomass in broomrape. In total, results suggested that PrSAI1 and, possibly, PrCWI are suitable targets for gene silencing strategies.

کلیدواژه‌ها [English]

  • Osmotic adjustment
  • Sucrose
  • Enzyme activity
  • reducing sugars
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