عنوان مقاله [English]
Broomrape is an obligate parasite in many economically important crops such as tomato. Success in controlling broomrape is rare hence an understanding of sucrose utilization mechanisms as a critical process for parasite provides developing specific control strategies. This parasite acts as a competitive sink for sucrose, utilizes it in the tuber and converts it to glucose and fructose. Invertases are the major enzymes in sucrose degradation and osmotic adjustment in the parasite. In this paper, the activity of two invertases (PrSAI and PrCWI) involved in different developmental stages of some broomrape species (Phelipanche aegyptiaca, P. ramosa and P. nana) on tomato was studied. Results showed that activity of both mentioned enzymes were detected in all of the developmental stages in the broomrape species; although the PrSAI activity was always higher than the PrCWI activity. The activities of PrSAI and PrCWI was increased and decreased, respectively, as progressing of the developmental stages of broomrape. Enzymes activity and protein and sugar amounts in Egyptian broomrape were high at the early stages and in branched broomrape in the late stages, the first case accorded to the earlier emergence of broomrape on the host and the later one accorded to more shoots and higher biomass in broomrape. In total, results suggested that PrSAI1 and, possibly, PrCWI are suitable targets for gene silencing strategies.
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