ارزیابی تحمل به شوری دورقم گلرنگ با استفاده از روابط یونی و شاخص‌های بیوشیمیایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار _ دانشگاه شیراز

2 دانشکده کشاورزی دانشگاه شیراز

3 دانشکده کشاورزی.دانشگاه شیراز

4 دانشگاه شیراز

چکیده

به‌منظور ارزیابی تحمل به شوری دو رقم گلرنگ (Carthamus tinctorius L.) و شناسایی مهم‌ترین شاخص‌های موثر بر تحمل به شوری، آزمایش گلخانه‌ای به ‌صورت فاکتوریل در قالب طرح پایه کاملاً تصادفی با دو فاکتور شوری آب آبیاری در 4 سطح (2، 4، 8 و 12 دسی‌زیمنس بر متر) و ارقام گلرنگ (صفه و زنده‌رود) در چهار تکرار انجام شد. با افزایش شوری از 2 به 4، 8 و12 دسی‌زیمنس بر متر، وزن خشک بوته رقم صفه به میزان13، 30 و 58 درصدکاهش یافت در حالی‌که این مقدار در رقم زنده‌رود به ترتیب 9، 28 و 40 درصد بود. رقم زنده‌رود در کلیه سطوح شوری بیشترین مقدار کلروفیل a،b، شاخص سبزینگی و فعالیت آنزیم‌های پراکسیداز و سوپراکسید دیسموتاز را به خود اختصاص داد. درصد افزایش سدیم ریشه و شاخساره (ساقه و برگ)، پرولین و مالون دی‌آلدئید در رقم صفه بیشتر از رقم زنده‌رود بود. نتایج رگرسیون نشان داد وزن خشک بوته با غلظت سدیم برگ، سدیم ریشه و پرولین دارای همبستگی منفی و معنی‌دار بود و با نسبت پتاسیم به سدیم، فعالیت آنزیم‌های پراکسیداز و سوپراکسید دیسموتاز و کلروفیل a همبستگی مثبت و معنی‌دار نشان داد. بطور کلی غلظت سدیم ریشه و شاخساره (ساقه و برگ) و فعالیت آنتی‌اکسیدانت‌های پراکسیداز و سوپراکسید دیسموتاز می‌توانند به عنوان شاخص‌های کارآمد در شناسایی ارقام متحمل گلرنگ مورد استفاده قرار بگیرند

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of salinity tolerance in two safflower cultivars using ions relations and biochemical traits

نویسنده [English]

  • Ehsan Shakeri 4
چکیده [English]

To investigate salinity tolerance in two safflower cultivars and identify the most important traits affecting salinity tolerance in safflower cultivars (Carthamus tinctorius L.), a greenhouse experiment was conducted as factorial arrangement based on completely randomized design with four replications. Treatments were irrigation water salinity (2, 4, 8 and 12 dS/m) and safflower cultivars (Sofeh and Zendehrud). Plant dry weight in Sofeh cultivar decreased 13, 30 and 58% respectively, while, plant dry weight of Zendehrud cultivar reduced 9, 28 and 40% under salinity levels. The higher concentration of chlorophyll a, b, SPAD, peroxidase and superoxide dismutase activity was found in Zendehrud cultivar under all salinity levels. Enhancement of Na+ concentration in shoot and root, proline and malondialdehyde was greater in Sofeh cultivar compared to Zendehrud cultivar. Linear regression analysis revealed significant and negative relationships between plant dry weight with Na+ concentration in leaf and root and proline content. Plant dry weight was significantly and positively associated with K+/Na+ ratio, peroxidase, superoxide dismutase and chlorophyll a. In general, it was concluded that Na+ concentration in root, shoot (stem + leaf), peroxidase and superoxide dismutase activity can be used as reliable indicators in identifying salinity tolerant cultivars of safflower

کلیدواژه‌ها [English]

  • Antioxidant
  • K+/Na+ Ratio
  • Plant dry weight
  • proline
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