مطالعه تاثیر تنش کم‌آبی برخی صفات فیزیولوژیکی و بیوشیمیایی چند ژنوتیپ سورگوم علوفه‌ای

نوع مقاله : مقاله پژوهشی

نویسندگان

دانشگاه محقق اردبیلی

چکیده

این پژوهش به منظور بررسی اثر تنش کم‌آبی بر فعالیت برخی آنزیم‌های آنتی‌اکسیدان، شاخص‌های سازگاری و رنگدانه‌های فتوسنتزی ژنوتیپ‌های سورگوم علوفه‌ای به‌صورت فاکتوریل و بر اساس طرح کاملاً تصادفی با سه تکرار در شرایط گلخانه‌ای در دانشگاه محقق اردبیلی در سال 1393 اجرا شد. تیمارهای آزمایش شامل چهار سطح آبیاری (85، 65، 45 و 25 درصد ظرفیت زراعی) و سه ژنوتیپ سورگوم علوفه‌ای (KFS2، KFS6، KFS17) بودند. فعالیت کاتالاز، پراکسیداز، پلی‌ فنل‌ اکسیداز و قندهای محلول، پرولین و کارتنوئیدها در تنش شدید (25 درصد ظرفیت زراعی) افزایش یافتند، در‌حالی‌که محتوای کلروفیل a و کلروفیل b کاهش پیدا کرد. ژنوتیپ KFS6 دارای بیشترین میزان کلروفیل a، کلروفیل b و کلروفیل کل بود؛ اما بیشترین بیوماس، پرولین، قندهای محلول، فعالیت آنزیم پراکسیداز، پلی فنل اکسیداز و میزان کارتنوئیدها در ژنوتیپ KFS2 مشاهده شد. بیوماس با کلروفیل‌ها همبستگی مثبت و با کارتنوئیدها، پرولین، قندهای محلول، کاتالاز، پراکسیداز و پلی فنل اکسیداز دارای همبستگی منفی بود. در بین شاخص‌های فتوسنتزی، کلروفیل a و در بین شاخص‌های سازگاری، پراکسیداز بیشترین سهم را در پیش‌بینی بیوماس داشتند. همچنین این شاخص‌ها در ژنوتیپKFS2، سهم بیشتری در پیش‌بینی بیوماس داشتد. به طور کلی می‌توان ژنوتیپ KFS2 را به‌عنوان ژنوتیپ سازگار به تنش کم‌آبی در مقایسه با دو ژنوتیپ‌ دیگر معرفی کرد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of water deficit stress on some physiological and biochemical traits of forage sorghum genotypes

چکیده [English]

This study was performed to investigate the effect of water deficit stress on the some activities antioxidant enzymes, photosynthetic pigments and forage yield of sorghum genotypes. This experiment was conducted to factorial based on completely randomized design with three replications in greenhouse of University of Mohaghegh Ardabili in 2013. Treatments consisted of irrigation at four levels (85, 65, 45 and 25% of field capacity) and three genotypes of forage sorghum (KFS2, KFS6, and KFS17). Catalase, peroxidase and polyphenol oxidase enzymes and soluble sugar content, proline and carotenoids, increased in severe stress (25% field capacity) and decreased chlorophyll a and chlorophyll b. KFS6 genotype had the highest chlorophyll a, chlorophyll b, and total chlorophyll, however, the maximum biomass, proline, soluble sugars, peroxidase and polyphenol oxidase activity and carotenoid were observed by KFS2 genotype. Also positive correlation were observed between the biomass production and carotenoid and chlorophyll content, proline, soluble sugar, catalase, peroxidase and negative correlation between biomass and polyphenol oxidase. Between photosynthetic indices, chlorophyll a, between adjustment indices, peroxidase activity had maximum contribution to prediction biomass. Also in KFS2 genotype except carotenoid and proline all traits had highest correlation with biomass. In general KFS2 genotypes introduced tolerant to water stress as compared with the other genotypes.

کلیدواژه‌ها [English]

  • Catalase
  • Peroxidase
  • photosynthesis
  • proline
  • Biomass

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سابقه مقاله

  • تاریخ دریافت: 30 آبان 1394
  • تاریخ بازنگری: 01 اسفند 1396
  • تاریخ پذیرش: 10 خرداد 1396
  • تاریخ انتشار: 01 اسفند 1396

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