تأثیر محلول پاشی اسیدسالیسیلیک بر برخی شاخص های فیزیولوژیکی چغندرقند در شرایط تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد زراعت، گروه زراعت و اصلاح نباتات، دانشکدة کشاورزی دانشگاه شهید چمران اهواز

2 استادیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی دانشگاه شهید چمران اهواز

3 دانشیار، گروه زراعت و اصلاح نباتات، دانشکدة کشاورزی دانشگاه شهید چمران اهواز

چکیده

به منظور بررسی تأثیر اسید سالیسیلیک بر برخی شاخص­های فیزیولوژیکی چغندرقند در شرایط تنش شوری برای شناسایی صفات مربوط به تحمل شوری، آزمایشی به صورت اسپلیت فاکتوریل در قالب طرح پایة بلوک­های کامل تصادفی با سه تکرار به صورت گلدانی، در سال زراعی 1392-1391 در مزرعة تحقیقاتی دانشگاه شهید چمران اهواز انجام شد. عامل‌های مورد بررسی شامل اسید سالیسیلیک با غلظت‌های0 (شاهد)، 5/0 و 1 میلی‌مولار به عنوان کرت اصلی، تنش شوری در دو سطح 0 (شاهد) و 150 میلی‌مولار و دو رقم چغندرقند شریف و جلگه بود. محلول­پاشی برگی در مرحلة 4 برگی، همزمان با تنش شوری انجام شد. نتایج نشان داد که تیمار اسید سالیسیلیک افزون بر افزایش معنی­دار سطح برگ، سبزینة (کلروفیل) برگ، هدایت روزنه‌ای، عملکرد کوانتومی نظام نوری (فتوسیستم) دو، بیشینه عملکرد کوانتومی نظام نوری دو، کل قندهای محلول و پرولین، موجب کاهش نفوذپذیری نسبی غشا نسبت به تیمار شاهد در شرایط تنش شوری شد. همچنین تیمار تنش شوری موجب کاهش معنی‌دار هدایت روزنه‌ای، سبزینة برگ، عملکرد کوانتومی نظام نوری دو و بیشینه عملکرد کوانتومی نظام نوری دو شد، درحالی­که نفوذپذیری نسبی غشا، کل قندهای محلول و پرولین به طور معنی­داری افزایش یافت. بنابراین با توجه به نتایج به‌دست‌آمده به نظر می­رسد استفاده از تیمار اسید سالیسیلیک در گیاه چغندرقند در شرایط تنش شوری با تحریک رشد و سوخت‌وساز، موجب تحمل به تنش شوری می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of foliar application of salicylic acid on some physiological traits of sugar beet in salt stress conditions

نویسندگان [English]

  • Maryam Mohammadi Cheraghabadi 1
  • Habibollah Roshanfekr 2
  • Payman Hasibi 2
  • Mousa Meskarbashi 3
1 M.Sc. Student, Department of Agronomy and Plant Breeding, Shahid Chamran University, Ahvaz, Iran
2 Assistant Professor, Department of Agronomy and Plant Breeding, Shahid Chamran University, Ahvaz, Iran
3 Associate Professor, Department of Agronomy and Plant Breeding, Shahid Chamran University, Ahvaz, Iran
چکیده [English]

To investigate the effect of salicylic acid (SA) on salinity tolerance of two sugar beet cultivars, this experiment was conducted as split factorial based on a randomized complete block design with three replications during growing seasons of 2012 and 2013 in Research Farm of Shahid Chamran University. The studied factors were three levels of SA (0 mM, 0.5 mM and 1 mM), two levels of salinity (150 mm sodium chloride and control) and two sugar beet cultivars (Jolgeh and Shariff). SA was applied as foliar spray along with salinity in the 4-leaf stage. Results showed that foliar application of SA caused significant increase of leaf area (LA), chlorophyll, stomatal conductance, ФPSІІ, Fv/Fm, proline and total soluble sugars, and decrease membrane permeability compared to control under salt stress conditions. Salinity caused a significant decrease in LA, Chlorophyll, stomatal conductance, ФPSІІ and Fv/Fm, while increase proline, total soluble sugars and membrane permeability. Therefore, according to the results of this research, it seems, foliar application of salicylic acid could enhance sugarbeet growth and increases resistance to salt stress.

کلیدواژه‌ها [English]

  • membrane permeability
  • proline
  • salinity tolerance
  • Quantum yield
Aftab, T., Khan, Idrees, M.M.A., Naeem, M. & Moinuddin, M.  (2010). Salicylic acid acts as potent enhancer of growth, photosynthesis and artemisinin production in Artemisia annua L. Journal Crop Scibiotech, 13, 183-188.
Arnao, M.B., Cano, A., Hernandez-Ruiz, J., Garcia- Canovas, F. & Acosta, M. (1996). Inhition by L-ascorbic acid and other antioxidants of the 2,2-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) oxidation catalyzed by Peroxidase: a new approach for determining total antioxidant status off foods. Journal of Analytical Biochemistry, 236, 255-261.
Asadinasab N, Hassibi P, Roshanfekr H. & Meskarbashee, M. (2013). Study on some physiological and morphological responses of three varieties of sugar beet (Beta vulgaris L.) to salinity. Crops Journal, 15, 94-79. (in Farsi)
Baiat, H., Mardani, H., Aroei, H. & selahvarzi, Y. (2012). Effect of salicylic acid on morphological and physiological characteristics of cucumber seedlings (Cucumic sativus cv. SuPer Dominus) under drought stress. Journal of Plant Production Research, 18(3), 260-253. (in Farsi)
Bates, I.S., Waldern, R.P. & Tear, I.D. (1973). Rapid determination of free proline for water stress studies. Journal of Plant and Soil, 39, 205-207.
Claussen, W. (2005). Proline as a measure of stress in tomato plant. Journal of Plant Science, 168, 241-248.
Hale, M. C. & Oreuh, D. M. (1998). The physiology of plant under stress. Jon Wiley & Sons, London. Journal of Agricultural Science, 10, 165-173.
Hamada, A.M. &  Elnay, A.E. )1994(. Effect of NaCl  Salinity on growth, Pigment and mineral element  contents, and gas exchange of broad bean and Pea Plants. Journal of Biological Plantarum, 36, 75-81.
He, Y., Liu, Y., Cao, W., Huai, M., Xu, B. & Huang, B. (2005). Effects of salicylic acid on heat tolerance associated with antioxidant metabolism in Kentuky Blue grass. Journal of Crop Science Direct,  45, 988-995.
Heydari sharif abad, H. (2002). Plant and salt stress. First edition. Forest and Rangeland Research Publication. p 199. (in Farsi)
Hussein, M.M. Balbaa, L.K. and Gaballah, M.S.  (2007). Salicylic acid and salinity effect on growth of maize plants. Research Journal of Agriculture and Biological Sciences. 3: 321-328.
Joodmand, M., Hajibland, R. and Fotoohi, K. (2008). Investigate of biochemical characterization of some varieties of sugar beet in salt stress conditions. Master's thesis of Plant Breeding, Tabriz University, Iran. (In Farsi).
Khan, W., Prithiviraj, B. & Smith, D.L. (2003). Photosynthetic response of corn and soybean to foliar application of salicylates. Journal of Plant Physiology, 160, 485-492.
Khodary, S.E.A. (2004). Effect of salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt-stressed maize Plants. International Journal of Agriculture and Biology, 6, 5-8.
Khoshbakht, D., Ramin, AA. & Bagbanha, M. (2013).  Reduce the effect of salt stress by application of salicylic acid in bean. Journal of Production of Crops and Garden, 5(2), 165-173. (in Farsi)
Khosravi, s., Baghizadeh, A. & Nezami Mohammad, T. (2011). The salicylic acid effect on the Salvia officianlis L. sugar, protein and proline contents under salinity (NaCl) stress. Journal of Stress Physiology and Biochemistry, 7, 80-87.
Kiarostami, KH., Abdolmaleki, N. & Heydari, M. (2013). Effect of salicylic acid on canola salinity stress reduction. Plant Biology, 12(4), 69-82. (in Farsi)
Maxwell, K. & Johnson, GN. (2000). Chlorophyll fluorescence – a practical guide. J Exp Bot, 51, 659-668.
Mohammadi, M., Khazaei, Z., Sayari, M. & Seyedi, M. (2012). Effect of salicylic acid on resistance to salt stress in Lettuce. Seventh Congress of Horticultural Sciences, Isfahan University of Technology. (in Farsi)
Nazarbeygi, A. & Lari Yazdi, H. (2010). Effects of different concentrations of salt, salicylic acid and gibberellic acid on proline content and chlorophyll in two varieties of oilseed rape. National Conference on Water, Soil, Plant and Agricultural Mechanization, 24(3), 211-240. (in Farsi)
Niazi, BH., Athar, M. & Rozema, J .(2004). Salt tolerance in the fodder beet and sea beet: Analysis of Biochemical relations. Bulg Journal of Plant Physilogy, 30(1-2), 78-88.
Ober, ES., Bloa, ML., Clark, CJA., Royal, A., Jaggard, KW. & Pidgon, JD. (2005). Evaluation of Physiological traits as indirect selection criteria for drought tolerance in sugar beet. Field CroPs Research, 91, 231-249.
Orcutt, D. M. & Nilsen, E. T. (2000). The physiology of plants under stress, soil and biotic factors. 1st edition. John Wiley and Sons, New York.
Parida, A. K. & Das, A. B. (2005). Salt tolerance and salinity effects on Plants: areview. Ecotoxicology and Environmental Safety, 60, 324-349.
Raskin, I. (1992). Role of salicylic acid in plants. Journal of Plant physiologyand Plant Molecular Biology, 43, 439-463.
Sairam, R. K., Srivasta, G. C., Agarwal, S. & Meena, R. C. (2005). Difference in antioxidant activity in response to salinitystress in tolerant and suscePtible wheat genotyPes. Journal of Biologia Plantarum, 49(1), 85-91.
Shaw, B., Thomas, T.H. & Cooke, D.T. (2002). Response of sugar beet (Beta vulgaris L.) to drought and nutrient deficiency stress. Plant Growth Regulators, 37, 77-83.
Shlegil, H.G. (1986). Die verwertung organgischer souren durch chlorella lhncht. Journal of Plant Sciences, 41, 47-51.
Singh, B. & Usha, K. (2003). Salicylic acid induced physiological and biochemical in wheat seedling under water stress. Journal of Plant Growth Regulation, 39, 137-141.
Tasgin, E., Atici, Q. & Nalbantoglu, B. (2003). Effects of salicylic acid and cold onfreezing tolerance in winter wheat leaves. Journal of Plant Growth Regulation, 41, 231-236.
Yassen, B.Y. & Jurgees, G.A. (1998). The response of sugar beet leaf growth and its ionic composition to sodium chloride. Journal Agriculture and water Resource Research soil and water Resources, 7(1), 47-59.
Yusuf, M., Aiman Hasan, S., Ali, B., Hayat, S., Fariduddin, Q. & Ahmad, A. (2008). Effect of salicylic acid on salinity-induced changes in Brassica juncea. Journal of Integrative Plant Biology, 50, 1096-1102.
Zhao, H. J., Lin, X. W., Shi, H. Z. & Chang, S. M. (1991). The regulating effects of phenolic compounds on the Physiological characteristics and yield of soybeans. Journal of ActaAgronomica Sinica, 21, 351-255.