تجزیة QTL برای برخی از مشخصه‌های جوانه‌زنی در رگه‌های خالص نوترکیب آفتابگردان در تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، دانشکدة کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات

2 کارشناس ارشد بیوتکنولوژی، دانشکدة کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات

چکیده

به منظور بررسی تغییرپذیری‌های ژنتیکی، شناسایی و مکان‌یابی ژن‌های کنترل‌کنندة صفات کمی  (QTL)مشخصه (پارامتر)های جوانه‌زنی بذر تحت تنش شوری در گیاه آفتابگردان، آزمایشی شامل هشتاد ژن‌نمون (ژنوتیپ) از یک جامعة خویش‌آمیختة (اینبرد) نوترکیب (RIL) آفتابگردان، ناشی از تلاقی دو رگه یا لاین (PAC2 x RHA266) در یک طرح فاکتوریل در دو شرایط کنترل و تنش شوری در سه تکرار و در شرایط آزمایشگاهی کنترل‌شده در مجتمع آزمایشگاهی دانشگاه آزاد واحد علوم و تحقیقات تهران اجرا شد. صفات مورد بررسی شامل زمان لازم برای آغاز جوانه‌زنی بذرها، زمان 50 درصد جوانه‌زنی، زمان بیشترین جوانه‌زنی  و درصد جوانه‌زنی بودند. نتایج گویای معنی‌دار بودن تنش شوری و تأثیر ژن‌نمون‌ها در همة صفات اندازه‌گیری‌شده بود. اگرچه تنش شوری تأثیر منفی روی جوانه‌زنی نشان داد، اما معنی‌دار بودن تأثیر ژن‌نمون در تنش نشان‌دهندة رفتار متفاوت ژن‌نمون­ها در پاسخگویی به تنش شوری بود. در این تحقیق، QTLهای "1.T50%G.16.1" و "2.T50%G.17.1" کنترل‌کنندة زمان 50 درصد جوانه‌زنی بذر در شرایط کنترل و تنش شوری، به عنوان QTLهای بزرگ‌تأثیر معرفی شدند. همچنین QTLهای (2.TSG.16.1) و (1.TSG.16.1) کنترل‌کنندة زمان آغاز جوانه‌زنی بذر وQTL های (2.PSG.12.1)، (1.PSG.12.1)، (2.PSG.12.3) و (1.PSG.12.2) کنترل‌کنندة درصد جوانه‌زنی بذر به عنوان QTLهای ساختاری معرفی شدند. از مکان‌های کروموزومی و نشانگر (مارکر)های جفت (لینک)شده با آنها در روش گزینش به کمک نشانگر برای بهبود صفت جوانه‌زنی در شرایط شوری پس از آزمایش‌های و تکرارهای لازم می‌توان استفاده کرد.

کلیدواژه‌ها


عنوان مقاله [English]

QTL mapping for seed germination parameters in sunflower (Helianthus annuus L.) recombinant inbred lines under salt stress

نویسندگان [English]

  • Asa Ebrahimi 1
  • Amir Hossein Fartash 2
  • Ali Mafakheri 2
  • Zahra Saatian 2
1 Assistant Professor, Department of Biotechnology, College of Agriculture and Natural Resources, Islamic Azad University, Science and Research
2 Former M.Sc. Student, Department of Biotechnology, College of Agriculture and Natural Resources, Islamic Azad University, Science and Research
چکیده [English]

In order to assess the genetic variability for seed germination parameters under salt stress in sunflower and corresponding QTL locations, an experiment was conducted using a population of 80 recombinant inbred lines obtained from a cross between PAC2 and RHA266. The experiment was arranged under a randomized complete block design with three replications in control and saline conditions. Several germination parameters were measured including time to start germination (TSG), time to 50% germination (T50%G), time to maximum germination (TMG) and percentage of seed germination (PSG). Results showed highly significant variations among genotypes (RILs) for all traits. Salt stress had a negative effect on germination, however the significance of RIL × Condition interaction suggested that the RILs have different responses to salt stress. In this study, “1.T50%G.16.1” and “2.T50%G.17.1” QTLs (controlling T50%G under salt and control conditions, respectively) were introduced as large-effect QTLs. Moreover, “2.TSG.16.1” and “1.TSG.16.1” QTLs (controlling TSG) and also “2.PSG.12.1”, “1.PSG.12.1”, “2.PSG.12.3” and “1.PSG.12.2” QTLs (controlling PSG) were introduced as stable QTLs.

کلیدواژه‌ها [English]

  • germination
  • QTL
  • salt stress
  • sunflowers
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