ارزیابی تأثیر پوتریسین در القای تحمل به خشکی و تغییر فعالیت آنزیم‌های ضداکسنده در گیاه بابونۀ آلمانی (Matricaria Chamomilla L.)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 استاد گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 استادیار پژوهشکدۀ گیاهان و مواد اولیۀ دارویی دانشگاه شهید بهشتی

چکیده

هدف آزمایش بررسی و ارزیابی تأثیر محلول‌پاشی پوتریسین (پلی‌آمین) بر روی میزان فعالیت آنزیم‌های ضداکسنده (آنتی‌اکسیدان) در گیاه بابونۀ آلمانی تحت تنش‌خشکی بود. تیمارهای آزمایشی شامل دو سطح شرایط (رژیم) رطوبتی (87 و 43 درصد ظرفیت مزرعه‌ای)، محلول‌پاشی پوتریسین (غلظت‌های 0 و 1/0 میلی‌مولار) و ارقام بابونۀ آلمانی (بادگلد و اصلاح‌شدۀ مجاری) بود. این آزمایش به‌صورت فاکتوریل در قالب طرح بلوک‌های کامل تصادفی در سه تکرار انجام شد. تنش‌خشکی به کاهش محتوای پروتیین محلول برگ و افزایش فعالیت آنزیم‌های ضداکسنده شامل آسکوربات پراکسیداز (APX)، کاتالاز (CAT)، گایاکول پراکسیداز (GPX) و گلوتاتیون رداکتاز (GR) منجر شد. کاربرد پوتریسین نیز به تحریک فعالیت آنزیم‌های بالا انجامید (به‌ترتیب به افزایش 6/26، 17، 56/26 و 88/8 درصد در آنزیم‌های APX، CAT، GPX و GR منجر شد). درحالی‌که تأثیر معنی‌داری بر محتوای پروتیین محلول برگ نداشت. اثرگذاری‌های متقابل بین شرایط رطوبتی و محلول‌پاشی پوتریسین بر فعالیت آنزیم GR (در سطح احتمال 5 درصد) معنی‌دار بود. همچنین اثرگذاری‌های متقابل بین شرایط رطوبتی و رقم تنها در آنزیم GPX معنی‌دار بود. در مقایسۀ بین دو رقم، فعالیت آنزیم‌های GR و GPX در رقم بادگلد بالاتر بود ولی فعالیت آنزیم‌های APX ، CAT و محتوی پروتیین محلول برگ در رقم اصلاح‌شدۀ مجاری بالاتر بود.

کلیدواژه‌ها

عنوان مقاله [English]

Putrescine induces drought tolerance and alters the activities of antioxidant enzymes in growing chamomile plants (Matricaria Chamomilla L.)

نویسندگان [English]

  • Hossein Nazarli 1
  • Ali Ahmadi 2
  • Javad Hadian 3
1 Ph. D. Student, Department of Agronomy and Plant Breeding, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Department of Agronomy and Plant Breeding, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Assistant Professor, Medicinal Plants and Drugs Research Institute, University College of Agriculture, University of Shahid Beheshti, Tehran, Iran
چکیده [English]

The present study focused on the influence of Put treatment on antioxidant enzyme activity in chamomile seedlings under drought stress. Treatments included two moisture regimes (87and 43درصد of field capacity), putrescine application (0 and 0.1 mM), and two chamomile cultivars (Bodgold and Hungary breed seed). The experiment was conducted in a factorial design based on randomized complete blocks with three replicates. Drought reduced soluble protein content, and increased the activities of antioxidant enzymes, including ascorbate peroxidase (APX), catalase (CAT), guaiacol peroxidase (GPX) glutathione reductase (GR). Put treatment caused a significant increase in the activity of antioxidant enzymes, as compared to control (respectively 26.6, 17, 26.56 and 8.88درصد for APX, CAT, GPX and GR), while no significant effect was recognized so long as the soluble proteins content was taken into account. A significant association was observed between moisture regimes and Put in terms of GR activity (P < 0.05), while interaction between cultivar and moisture regimes was only significant as the variable of GPX was considered (P < 0.05). Interestingly, between two cultivars, Bodgold was found to contain higher GR and GPX activities compared to Hungary breed seed. While Hungary breed seed exhibited higher soluble proteins content, APX and CAT activities than the other one.

کلیدواژه‌ها [English]

  • Antioxidant Enzymes
  • Chamomile
  • Drought stress
  • Putrescine
  • soluble protein content

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  • تاریخ دریافت: 03 اسفند 1393
  • تاریخ بازنگری: 04 خرداد 1394
  • تاریخ پذیرش: 16 خرداد 1394
  • تاریخ انتشار: 01 تیر 1394

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