نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.
2 گروه بیوتکنولوژی کشاورزی، دانشکده کشاورزی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران.
3 گروه زراعت و اصلاح نباتات ، دانشکده کشاورزی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران.
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Drought stress is one of the most important limiting factors for plant growth and soil fertility, which causes crop yield reduction. In order to investigate the relative expression of genes related to drought stress tolerance, 4 genotypes (including two sensitive and two resistant to moisture stress) of bread wheat as factor A were subjected to a factorial experiment on basis randomized complete block design with three replications under normal and drought stress at the beginning of flowering stage as factor B in the research farm of Azerbaijan Shahid Madani University. The expression of genes in the examined samples was performed by semi-quantitative polymerase chain reaction in three replicates. Glutathione peroxidase gene (GAPDH) was used as housekeeping gene. Examining the relative expression level of antioxidant genes showed that the high tolerance of Mihan cultivar to drought stress can probably be attributed to the high activity of catalase and ascorbate peroxidase enzymes and the high tolerance of Arum cultivar to the higher activity of antioxidant enzymes proline synthetase, peroxidase and attributed to superoxide dismutase. Considering that the antioxidant genes catalase, ascorbate peroxidase, proline synthetase, peroxidase and superoxide dismutase in tolerant cultivars showed a significant increase in expression under stress and normal conditions compared to sensitive cultivars under the same stress and normal conditions, so this Genes may induce tolerance to drought stress in plants under stress conditions.
کلیدواژه‌ها [English]
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