اثر دما بر متابولیت ها و بیان برخی از ژن‌های مسیر بیوسنتز تیمول و کارواکرول در آویشن باغی (Thymus vulgaris)

نوع مقاله : مقاله پژوهشی


1 دانشیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، گروه زراعت و اصلاح نباتات.

2 دانشجوی دکتری، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، گروه زراعت و اصلاح نباتات.

3 دانشگاه شهید بهشتی


عوامل محیطی مختلف سبب تغییر در میزان و نوع متابولیت‌های ثانویه در گیاهان  می شوند. به‌منظور بررسی اثر دما بر متابولیت‌های ثانویه و بیان ژن‌های مسیر بیوسنتزی تیمول و کارواکرول در آویشن باغی، آزمایشی با سه تیمار دمایی گرما، سرما و شرایط طبیعی در اتاقک رشد، به مدت یک ماه اجرا شد. میزان ترکیبات اسانس با استفاده از دستگاه Gc/MS و  Gc و میزان بیان ژن­های TvTps1، CYP178، CYP180 و CYP181 به‌وسیله qRT-PCR اندازه گیری شد. اگرچه اعمال هر دو تیمار دمایی گرما و سرما، ترکیبات مونوترپنی فنولی (تیمول و کارواکرول) را نسبت به شرایط طبیعی افرایش داد، دمای بالا سبب افزایش معنی‌دار میزان کارواکرول و  دمای پائین، سبب افزایش معنی دار مقدار تیمول شد. تیمارهای دمایی در مقایسه با شرایط طبیعی، میزان گاماترپنین را کاهش داد. تجزیه بیان ژن‌ها نشان داد که اعمال تیمار سرما، سبب افزایش میزان بیان ژن‌های TvTps1 و CYP178 و تیمارگرما موجب کاهش آن‌ها شد. میزان بیان ژن‌های CYP180 و CYP181 در هر دو شرایط سرما و گرما افزایش نشان داد؛ اگرچه میزان افزایش در شرایط گرما، بسیار بیشتر از تیمار سرما بود. به‌طورکلی نتایج نشان داد که دمای محیط نقش بسزایی در میزان متابولیت­های آویشن دارد و احتمالا مناطق گرم تر برای تولید کارواکرول و مناطق سردتر برای تولید تیمول مناسب تر هستند.


عنوان مقاله [English]

The impact of temperature on metabolites and the expression of genes involved in thymol and carvacrol biosynthesis pathway in Thymus vulgaris

نویسندگان [English]

  • Valiollah Mohammadi 1
  • Behnam Mondak 2
  • Javad Hadian 3
  • Hassan Zeinali Khanghah 1
1 Agronomy and Plant Breeding Dept. University of Tehran
2 Agronomy and Plant Breeding Dept. University of Tehran
چکیده [English]

Environmental factors make changes in the amount and type of secondary metabolites. To study the effects of temperature regimes on secondary metabolites and the expression of genes involved in biosynthesis pathway of thymol and carvacrol in thyme, an experiment was conducted in a growth chamber for one month with three thermal treatments including heat, cold and normal temperatures. Essential oil compounds were measured by Gc / MS and Gc and the expression of TvTps1, CYP178 and CYP180 genes assayed by qRT-PCR. Both heat and cold temperatures increased the phenolic monoterpen compounds (thymol and carvacrol) compared to normal condition. High temperature led to significant increases in carvacrol while low temperature resulted in a great increase in the amount of thymol. Gamaterpenin level was declined due to thermal treatments. Gene expression analysis revealed that the cold condition increased the expression of TvTps1 and CYP178 genes, while heat decreased the expressions. CYP180 and CYP181 were highly expressed under both cold and heat conditions, although the amount of expression was much higher under high temperature. Generally, the results indicated that the environment temperature played a key role in thyme metabolites type and content. It could be concluded that the regions with high and low temperatures were suitable for carvacrol and thymol production, respectively.

کلیدواژه‌ها [English]

  • Carvacrol
  • cold
  • CYP
  • effect
  • heat
  • thyme
  • thymol

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دوره 51، شماره 1
اردیبهشت 1399
صفحه 195-205
  • تاریخ دریافت: 29 مهر 1397
  • تاریخ بازنگری: 08 اسفند 1397
  • تاریخ پذیرش: 17 فروردین 1398
  • تاریخ اولین انتشار: 01 اردیبهشت 1399