تأثیر تلقیح بذر با باکتری های محرک رشد بر برخی شاخص‌های جوانه‌زنی، بیوشیمیایی و محتوای برخی عناصر رازیانه (Foeniculum vulgare L.) تحت تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 عضو هیأت علمی دانشگاه یاسوج

2 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه یاسوج، یاسوج، ایران

3 دانشکده کشاورزی دانشگاه یاسوج

چکیده

با توجه به توسعه کشت گیاهان دارویی و روند افزایش اراضی شور و همچنین اهمیت روزافزون کاربرد کودهای زیستی در راستای نیل به کشاورزی پایدار، مطالعه حاضر جهت بررسی واکنش شاخص‌های جوانه‌زنی و بیوشیمیایی گیاه رازیانه به تلقیح با باکتری‌های محرک رشد در شرایط تنش شوری اجرا شد. آزمایش به صورت فاکتوریل دو عاملی در قالب طرح پایه کاملاً تصادفی در چهار تکرار با تلقیح بذور با باکتری در 5 سطح (PF2 و PF56 از سودوموناس فلورسنس، باسیلوس سابتیلیس، ازتوباکتر و عدم تلقیح) و تنش شوری در سه سطح (صفر، 4- و 8- بار) در آزمایشگاه دانشکده کشاورزی دانشگاه یاسوج در سال 1395 انجام شد. نتایج نشان داد که تنش شوری صفات محتوای پروتئین محلول، فعالیت آنزیم آسکوربات پراکسیداز، فسفر گیاهچه، پتاسیم گیاهچه، درصد جوانه‌زنی، صفات شاخص وزنی و طولی بنیه را کاهش داد و محتوای سدیم و نسبت سدیم به پتاسیم گیاهچه را افزایش داد. در هر سه سطح تنش شوری به ترتیب بیشترین و کمترین درصد جوانه‌زنی از بذرهای تلقیح شده با PF2 و بذرهای تلقیح نشده به‌دست آمد. در تنش 8- بار بیشترین پروتئین محلول (841/0 میلی‌مول بر گرم وزن تر بذر) و کمترین نسبت سدیم به پتاسیم (674/0 درصد) از سویه PF2و کمترین پروتئین محلول (531/0 میلی‌مول بر گرم وزن تر بذر) و بیشترین نسبت سدیم به پتاسیم (081/1درصد) از تیمار بدون تلقیح بدست آمد. به طور کلی تیمارهای زیستی از طریق افزایش فعالیت آنزیم‌های آنتی‌اکسیدانت و حفظ تعادل عناصر در بذر و گیاهچه در بهبود جوانه‌زنی گیاه رازیانه در شرایط تنش شوری مؤثر بود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The influence of seeds inoculation with Plant Growth Promoting Rhizobactria (PGPR) on some germination, biochemical indices and content of some elements in fennel (Foeniculum vulgare L.) under salinity stress

نویسندگان [English]

  • Massoumeh hoseinimoghadam 2
  • Ramin Piri 3
2 Department of Agronomy and Plant Breeding, faculty of agriculture, Yasouj University, yasouj, IRAN
3 College of africulture, yasouj university
چکیده [English]

Regarding the development of medicinal plants cultivation and the increase of saline lands, as well as the increasing importance of using biological fertilizers in order to achieve sustainable agriculture, the present study was conducted to investigate the response of germination and biochemical indices of fennel to inoculation with growth promoting rhizobacteria under salt stress conditions. This experiment was conducted as a factorial based on two factors in a completely randomized design with four replications by inoculating seeds with bacteria in 5 levels (PF2, PF56 of Pseudomonas fluorescent, Bacillus subtilis, Azotobacter sp. and non-inoculation) salinity stress in three levels (0, -4 and -8 bar imposed by NaCl) at laboratory of agriculture faculty of Yasouj University in 2015. The results showed that salinity stress decreased soluble protein content, ascorbate peroxidase enzyme activity, P content, K content, germination percentage, weight and length vigors, and increased Na content and Na/K ratio. In all three levels of salinity stress, the highest and lowest germination percentage was obtained from seeds inoculated with PF2 strain and non-inoculated seeds, respectively. In salinity level of -8 bar, the highest soluble protein content (0.841 mM. g-1 FW) and the least ratio Na/K (0.674 %) was obtained from PF2 strain and also lowest soluble protein content (0.0531 mM. g-1 FW) and the highest Na/K ratio (1/081) was related to control (0bar) treatment. In general, biological treatments were effective in improving germination and increasing the activity of antioxidant enzymes under salinity stress conditions in fennel.

کلیدواژه‌ها [English]

  • Antioxidant Enzymes
  • Plant Growth Promoting Rhizobacteria
  • Salinity stress
  • medicinal plants
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