تأثیر تنش خشکی اعمال شده توسط پلی اتیلن گلایکول بر خصوصیات فیزیولوژیک ومورفولوژیک جوانه زنی بذور عدس(Lens culinaris.) به منظور گزینش ژنوتیپ های متحمل به خشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 هئیت علمی

2 دانشجو دکتری

3 دانشجوی دکتری

چکیده

تتنشها ی محیطی، به ویژه تنش خشکی، از مهمترین عوامل کاهش و اختلال در مراحل مختلف رشد و نمو گیاهی، به خصوص جوانه زنی، در مناطق خشک و نیمه خشک ایران است. بدین منظور آزمایشی، به صورت فاکتوریل، در قالب طرحی کاملاً تصادفی، با سه تکرار در شرایط آزمایشگاه به اجرا درآمد. در این آزمایش از 49 ژنوتیپ عدس و چهار تیمار خشکی شامل صفر( شاهد)،3- ،6- و 9- بار استفاده شد. صفات مورد بررسی شامل درصد جوانه زنی، سرعت جوانه زنی، وزن خشک ساقه چه و ریشه چه، ،بنیه بذر و ضریب آلومتری بودند. نتایج حاصل از تجزیه واریانس و مقایسه میانگین نشان داد که در اکثر صفات مورد بررسی، تأثیر سطوح پتانسیل، ژنوتیپ و اثر متقابل آنها معنی دار بوده و کاهش پتانسیل باعث کاهش مقدار صفات گردیده است. ضرایب همبستگی ساده بین صفات نشان داد که درصد جوانه‌زنی بیشترین میزان همبستگی را با بنیه بذر (**85/0r=) و سرعت جوانه‌زنی (**80/0r=) دارد. همچنین نتایج حاصل از تجزیه به عامل ها نشان داد که دو عامل جوانه‌زنی و همچنین صفات مربوط به ساقه‌چه حدود 67 درصد تغییرات را توجیه می کنند. در مجموع نتایج بیانگر وجود تنوع ژنتیکی قابل توجه در بین ژنوتیپ‌ها بود و با توجه به صفات مورد بررسی،. ژنوتیپ های 6، 4 و 48 از تحمل بالایی و ژنوتیپ‌های 34، 28 و 45 از تحمل پایینی نسبت به خشکی برخوردار می‌باشند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of drought-induced stress by PEG6000 on physiological and morphological traits of Lentil (Lens culinaris.) seed germination in order to selection of drought tolerant genotypes

نویسندگان [English]

  • manijeh sabokdast nodehi 1
  • farshad salehi 2
  • ahmad rezaizadeh 3
چکیده [English]

Drought stress is one of the most important factors in arid and semiarid regions that lead to disorder in growth and development of plants especially at germination stage. Because of importance lentil, achieving genotype(s) that are capable of the drought stress at initiating growth stage is necessary. For this purpose, germination and plantlet growth of 49 genotypes were tested in drought stress conditions at four levels including 0 (control), -4, -8 and -12 Bar using PEG6000. This experiment was conducted at laboratory of Agronomy and Plant Breeding Department of University of Tehran in Karaj in 2016 as a factorial design in randomized complete block design (RCBD) with three replications. Traits that were studied including: germination percentage, germination rate, root length, shoot length, dry weight of roots and dry weight of shoots and allometry factor. Data were subjected to variance analysis and mean comparison. Results indicated that in most considered traits, effect of potential levels of genotypes and their interaction were significant. Result of simple correlation coefficient analysis indicated that germination percentage has the highest correlation with vigor germination (r=0.855) and speed germination (r=0.798). Factor analysis determined that two factors (germination and root traits) accounted 67% of the total variations among genotypes. In general, results represented that considerable genetic diversity was among genotypes. Regarding the studied traits, "6, 4 and 48" genotype had high tolerance to drought stress at germination stage, "34, 28” and “45" had low tolerance.

کلیدواژه‌ها [English]

  • Lentil
  • Drought stress
  • germination
  • Poly Ethylene Glycol (PEG)
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