بررسی تحمل به یخ‌زدگی در باقلا (Vicia faba L.) با استفاده از شاخص نشت الکترولیت‌ها

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زراعت دانشکده کشاورزی دانشگاه فردوسی مشهد

2 عضو هیات علمی پژوهشکده علوم گیاهی دانشگاه فردوسی مشهد

چکیده

کشت پاییزه گیاهان سرمادوست منجر به بهبود عملکردشان می‌شود، باوجود این‌در خصوص تحمل به سرمای توده‌های باقلای بومی جهت کشت پاییزه اطلاعات اندکی در دسترس می‌باشد. به این منظور آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با چهار تکرار تحت شرایط کنترل‌شده اجرا شد و طی آن دو توده باقلا (بروجرد و نیشابور)، در معرض دماهای یخ‌زدگی (صفر، 4-، 8-، 12-، 16-، 20-، 24- درجه سانتی‌گراد) قرار گرفتند. با کاهش دما درصد نشت الکترولیت‌ها در هر سه اندام برگ، ساقه و مریستم افزایش یافت، باوجود این در اندام برگ و مریستم کاهش دما تا دمای 12- درجه‌سانتی‌گراد تأثیر چندانی بر درصد نشت الکترولیت‌ها نداشت و با کاهش بیشتر دما درصد آن افزایش یافت و در دمای 20- درجه سانتی‌گراد به حداکثر رسید. در هر دو توده بروجرد و نیشابور بالاترین LT50el را اندام ساقه (به ترتیب 9/12- و 1/12- درجه‌سانتی‌گراد) داشت، درصورتی‌که دمای کشنده بر اساس 50 درصد نشت الکترولیت‌ها از برگ توده‌های بروجرد و نیشابور به ترتیب در دماهای 7/16- و 6/15- درجه‌سانتی‌گراد و در مریستم آن‌ها به ترتیب در دماهای 0/16- و 8/16- درجه‌سانتی‌گراد اتفاق افتاد. هرچند که بین درصد نشت الکترولیت‌ها از اندام‌های گیاه باقلا با درصد بقاء همبستگی منفی و معنی‌داری مشاهده شد، ولی دو اندام برگ و ساقه توده نیشابور، در LT50su مشابه با توده بروجرد (6/13- درجه‌سانتی‌گراد) درصد نشت الکترولیت‌های بیشتری داشتند، درحالی‌که در شرایط مذکور در توده بروجرد، درصد نشت الکترولیت‌ها از مریستم بیشتر بود. دو توده باقلا توانایی تحمل به دماهای یخ‌زدگی تا 12- درجه‌سانتی‌گراد را در شرایط کنترل‌شده نشان دادند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of freezing tolerance in faba bean (Vicia faba L.) using electrolytes leakage index

نویسندگان [English]

  • Alireza Hasanfard 1
  • Ahmad Nezami 1
  • Mohammad Kafi 1
1 Faculty of Agriculture, Ferdowsi University of Mashhad
چکیده [English]

Autumn planting leads to improve the yield of cool season crops, however there is little information about the cold tolerance in national faba bean landraces for autumn planting. For this purpose a study was conducted as factorial, based on completely randomized design with four replications under controlled conditions, and two faba bean landraces (Boroujerd and Neyshabour) exposed to freezing temperatures (0, -4, -8, -12, -16, -20, -24°C). Results showed that decreasing temperature leads to the increasing EL% in all organs, however decreasing temperature hadn't significant effect on EL% up to -12°C in leaf and meristem and EL% increased with reducing the temperature to reached to the maximum in -20°C, While in stem EL% increasing was started from -8°C and reached to the maximum in -16°C. In both of Boroujerd and Neyshabour landraces, stem had the highest LT50el (-12.9 and -12.1, respectively), while it was -16.7 and -15.6°C in leaf and -16.0 and -16.8°C in meristem of two mention landraces, respectively. Although there was negative and significant correlation between the EL% of faba bean organs with SU%, but leaf and stem in Neyshabour landrace in similar LT50su with boroujerd landrace (-13.6°C) had more EL%, while it was further in the meristem of Boroujerd landrace. Two faba bean landraces showed ability to tolerate freezing temperatures up to -12°C in controlled conditions.

کلیدواژه‌ها [English]

  • Cold acclimation
  • lethal temperature 50% of plants
  • meristem
  • stem
  • Survival
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