اثر باکتری‌های محرک رشد مقاوم به شوری جدا شده از ریزوسفر گیاهان شورپسند بر رشد و مقاومت گندم تحت تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی مولکولی پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری.

2 - استادیارگروه بیوتکنولوژی، دانشکده مهندسی انرژی و فناوری های نوین دانشگاه شهید بهشتی، تهران.

3 هیات علمی- موسسه تحقیقت اصلاح و تهیه نهال و بذر- سازمات تحقیقات آموزش و ترویج کشاورزی

4 - Professor, Biotechnologyand Genetics Research Institute of Tabarestan Sari University of Agricultural Sciences and Natural Resources, Sari, Iran

چکیده

به منظور بررسی اثر ریزوباکتری های محرک رشد گیاه بر مقاومت به شوری گندم در مرحله رشد رویشی، تعدادی جدایه از خاک‌های ریزوسفری گیاهان شورپسند، منطقه بیابانی اصفهان و گلستان با شوری بیش از 100 میلی مولار جداسازی و از نظر تحمل به تنش شوری، صفات حل کنندگی فسفات، تولید اکسین، سیدروفور و سیانید هیدروژن غربالگری شدند. از بین 50 جدایه بدست آمده،10 جدایه متحمل به شوری بین20-5 درصد بوده و تعداد 8 جدایه قادر به انحلال فسفات نامحلول (2012-188) میکرو گرم بر میلی لیتر و 6 جدایه هم مولد اسید ایندول-3-استیک (1/4-7/121) میکرو گرم بر میلی لیتر بودند. دو جدایه سیدروفور تولید نموده و هیچ یک از جدایه ها توان تولید سیانید هیدروژن را نداشتند. اثر 10 جدایه باکتری متحمل به شوری و دو سطح صفر و 100 میلی مولار شوری بر روی رشد گندم تحت تنش شوری 100 میلی مولار در مراحل اولیه رشد رویشی رشد انجام شد. نتایج تاثیر باکتریهای محرک رشد بر وزن خشک ریشه و ساقه، وزن خشک کل، میزان اتیلن در شرایط تنش شوری روی رقم قدس گندم نشان داد که تلقیح با باکتریهای منتخب در تعدیل اثرات مضر شوری در دوره رشد رویشی گندم موثر بود. در تلقیح با جدایه 88 (بهترین جدایه از نظر افزایش رشد در تنش شوری) وزن خشک کل (261درصد)، وزن خشک ساقه (390 درصد) و ریشه (270 درصد) در مقایسه با تیمار شاهد افزایش و میزان اتیلن (24درصد) کاهش یافت.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of salt-tolerant plant growth-promoting rhizobacteria isolated from rhizosphere of halophyte plants on growth wheat (Triticum aestivum L.) in saline environment

چکیده [English]

To study the efficiency of salt-tolerant plant growth-promoting rhizobacteria, on salt resistance of wheat plant (Triticum aestivum L) at growth stage, some strains isolated from the rhizosphere of halophyte plant rhizosphere which were collected from the deserts of four provinces (Isfahan, Yazd, Golestan and Hormozgan) of Iran. All strains were screened on their ability as salt tolerant, phosphate solubility, Auxin, siderophore and hydrogen cyanide (HCN) production. 10 isolates from a total of 50 isolates were salt tolerant to 5-20 percent salinity and have plant growth promoting (8 isolates isolates were able to solubilize insoluble phosphate and 6 isolates produced Indole-3-acetic acid (IAA). Only two isolates produced siderophore and none of the isolates had the ability to produce cyanide hydrogen. Effects of 10 salt tolerant bacteria and two salt treatments (zero and 100 mM) on growth of A pot experiment as a factorial experiment in a completely randomized design with three replications with two factors including bacteria (10 salt tolerant bacteria) and salinity in salinity levels (zero and 100 mM). The effect of PGPR on root and shoot dry weight, total dry weight, the amount of ethylene on Ghods wheat in saline conditions showed that the inoculation with selected bacteria in modulating the negative effects of salinity on growth period of wheat has been effective. Total dry weight (261%), shoot (390%) and root dry weight (270%) increased compared with control treatment and decreased levels of ethylene (24%) in salinity.

کلیدواژه‌ها [English]

  • Auxin
  • Ethylene
  • Plant growth promoting
  • salt
  • Wheat
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