بررسی تأثیر اندول استیک اسید و آبسیزیک اسید بر غده‌زائی در گیاه سیب‌زمینی (Solanum tuberosum)

نوع مقاله : مقاله پژوهشی

نویسنده

استادیار گروه زیست شناسی دانشگاه آزاد اسلامی واحد مرودشت

چکیده

در این پژوهش تأثیر هورمون‌های اندول استیک اسید (IAA) و آبسیزیک اسید (ABA) بر غده‌زائی در سیب‌زمینی رقم آگریا (Agria) در شرایط گلخانه‌ای بررسی شد. ریز غده‌های بدون عامل‌های بیماریزا به گلدان‌هایی با خاک مناسب و ضدعفونی شده منتقل شدند. گیاهچه‌ها در طرح فاکتوریل در مرحلۀ 7-8 برگی با غلظت‌های 0، 5 و 10 میکرومولار IAA و 0، 5/2 و 5 میکرومولار ABA محلول‌پاشی شدند. نتایج نشان داد، با کاربرد IAA سطح برگ کاهش ولی با کاربرد ABA سطح برگ افزایش پیدا می‌کند. همچنین با افزایش غلظت هر دو هورمون شمار برگ و شمار غده افزایش پیدا می‌کند. افزون بر این با افزایش غلظت ABA، وزن غده و وزن اندام‌های هوائی کاهش پیدا می‌کند. نتایج نشان داد، با افزایش IAA، سبزینۀ (کلروفیل) کل و کاروتنوئیدها افزایش ولی با افزایش ABA، میزان آن‌ها کاهش پیدا می‌کند. همچنین با افزایش IAA، فعالیت آنزیم‌های پراکسیداز و پلی فنل اکسیداز در برگ گیاهان یک روند کاهشی دارند درصورتی‌که با افزایش ABA روند فعالیت این آنزیم‌ها افزایشی است. به نظر می‌رسد که تأثیر هورمون‌های اکسین و آبسیزیک اسید روی غده‌زائی در سیب‌زمینی به‌صورت متضاد باشد، هرچند این تأثیر ناهمسازی (آنتی‌گونیستی) به‌صورت کامل (100درصد) نیست و به غلظت هورمون‌های به‌کاررفته و شرایط و مراحل رشد و اندام‌های مورد بررسی در گیاه بستگی دارد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effects of indole acetic acid and abscisic acid on the tuberization in potato (Solanum tuberosum)

نویسنده [English]

  • Mohammad Reza Hadi
Assistant Professor, Department of Biology, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran
چکیده [English]

This study was carried out to evaluate the effects of indole acetic acid (IAA) and abscisic acid (ABA) on the tuberization in potato (CV. Agria) under greenhouse conditions. The potato mini-tubers (without any infection) were planted in pots containing pasteurized soil. The 4 weeks-old plants (7-8 leaves) were treated by spraying the foliage with 0, 5 and 10 µM (IAA) and with 0, 2.5 and 5 µM ABA weekly for 3 weeks. The results showed that application of IAA decreased the leaf area, but application of ABA increased the leaf area. With increasing concentrations of both hormones also increased the leaves numbers and tubers numbers. In addition, by increasing the concentration of ABA reduced the shoot weight and tuber weight. The results showed that total chlorophyll and carotenoids increased in leaves with increase in IAA applied, but they are reduced by increasing ABA levels. Also, by increasing IAA, polyphenol oxidase and peroxidase activities in leaf is a downward trend, while with increasing ABA, the activity of these enzymes is a upward trend. It seems that the influences of hormones auxin and abscisic acid on the tuberization in potato as an antagonistic effect however, this effect is not completely (100%) and depends on applied concentrations of hormones, conditions and growth stages and plant organs examined.

کلیدواژه‌ها [English]

  • Auxin
  • Abscisic acid
  • Chlorophyll
  • Carotenoids
  • tuberization
  • Potato (Solanum tuberosum)
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