بررسی تغییرپذیری‌های الگوی بیان پروتئین در رقم‌های متحمل و حساس کلزا در شرایط تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

استادیار بخش کشاورزی، دانشگاه پیام نور، ایران

چکیده

به‌منظور بررسی سازوکار تحمل و حساسیت به تنش شوری در کلزا (Brassica napus L.)، رقم‌های SW5001 و Sarigol به ترتیب به‌عنوان رقم‌های متحمل و حساس به‌صورت آزمایش فاکتوریل در قالب طرح بلوک کامل تصادفی در چهار تکرار در گلخانه کشت شدند. نمونه‌های برگی، دو هفته پس از اعمال تنش شوری و در پایان مرحلۀ وردمانی (روزت) برداشت شدند. پروتئین از بافت برگی استخراج و الکتروفورز دوبعدی برای بررسی بیان پروتئین­ها در گیاهان شاهد و در شرایط تیمار شوری انجام شد. رنگ‌آمیزی ژل‌ها با آبی کوماسی و تجزیۀ لکه‌های پروتئینی با نرم‌افزار PDQuest انجام و لکه­های پروتئینی با استفاده از طیف‌سنجی (اسپکترومتری) جرمی و به روش MALDI TOF/TOF MS شناسایی شدند. شمار هفده لکۀ پروتئینی با اختلاف بیان معنی­دار بین گیاهان شاهد و تیمار تنش شوری، تشخیص داده شدند که از این شمار ده لکۀ پروتئینی بین دو رقم مشترک و شمار چهار و سه لکۀ پروتئینی به ترتیب به رقم متحمل و حساس اختصاص داشتند. پروتئین‌های مشترک شناسایی‌شده در گروه­های عملکردی شامل پروتئین‌های دخیل در واکنش نوری نورساخت (فتوسنتز)، چرخۀ کالوین، حذف پاداکسنده (آنتی­اکسیدانت)، انتقال پیام و پایداری ساختار پروتئین­ها طبقه‌بندی شدند. در مجموع در رقم متحمل SW5001 عامل‌های پایداری ساختار، حفظ کارایی سوخت‌وساز (متابولیسم) کربن و دفاع در برابر تنش اکسایشی در شرایط تنش به نحو مؤثری موجب ایجاد مقاومت شدند. در ضمن بیشترین نقطۀ قوت رقم متحمل با اتکا به پروتئین­های منحصربه‌فرد در قسمت واکنش نوری نورساخت بود و رقم حساس بیشترین آسیب را در چرخۀ کالوین متحمل شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Assessment of changes in protein expression pattern in tolerant and sensitive cultivars of rapeseed under salt stress

نویسندگان [English]

  • Marouf Khalili
  • Mohammad Reza Naghavi
Assistant Professor, Department of Agriculture, Payamme Noor University, Iran
چکیده [English]

To investigate the mechanisms of tolerance and sensitivity to salinity stress in canola (Brassica napus L.), two spring canola cultivars such as SW5001 as tolerant cultivar and Sarigol as sensitive cultivar were evaluated at two levels of salinity in factorial based on randomized complete block design with four replications using a hydroponic culture system into green house. Sampling of leaves was performed in the rosette stage and two weeks after starting of salt stress. Proteins were extracted from leaf tissues and two-dimensional electrophoresis for the study of expression of proteins in both control and salt stressed plants was performed. Staining with commassie brilliant blue and quantitative analysis of protein spot by PDQuest software was performed and protein spots were identified by mass spectrometry using MALDI TOF/TOF MS method. 17 protein spots with significant expression differences between control and treatment plants to salinity, were identified that Of these, 10 protein spots was common between two cultivars and four and three proteins were assigned only to tolerant and sensitive cultivars respectively. Common proteins identified were classified in the functional groups of photo reaction of photosynthesis, Calvin cycle, proteins involved in remove of antioxidant, signal transduction and structural stability of proteins. In generally, in the tolerant cultivar of SW5001, sustainability factors of structure, maintaining of carbon metabolism efficiency and defense against to oxidative stress under stress conditions were effectively creates resistance. The highest strength point tolerant cultivar to rely on the unique proteins was in the light reaction of photosynthesis and the most of damage suffered in sensitive cultivar related to Calvin cycle.

کلیدواژه‌ها [English]

  • 2D electrophoresis
  • Canola
  • responsive proteins to stress
  • tolerance to salt stress
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