تأثیر نانو محرک‌ها بر تولید و بیان ژن‌های دخیل در زیست‌ساخت آلکالوئیدها در دروایۀ شقایق شرقیPapaver orientale L.

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، گروه زراعت و اصلاح نباتات، دانشکدۀ کشاورزی دانشگاه تهران، کرج

2 استاد، گروه زراعت و اصلاح نباتات، دانشکدۀ کشاورزی دانشگاه تهران، کرج

چکیده

کشت بافت منبع بالقوه‌ای در جهت تولید متابولیت‌های ثانویه است. محرک (الیسیتور)ها نقش مهمی در القای سامانۀ دفاعی گیاهان و تولید متابولیت‌های ثانویه ایفا می‌کنند. الکالوئیدها گروهی از متابولیت‌های ثانویه و با ارزش اقتصادی هستند که استفاده از آن‌ها توسط انسان به عصر باستان باز می‌گردد. بسیاری از گونه‌های Papaver توانایی تولید آلکالوئیدهایی مانند پاپاورین، نوسکاپین، مورفین و کدئین را دارند. در این پژوهش تغییرپذیری بیان ژن‌های دخیل در زیست‌ساخت (بیوسنتز) این آلکالوئیدها در کشت دروایۀ (سوسپانسیون) یاخته‌ای Papaver orientale در فاصله‌های زمانی24 و 48 ساعت پس از اعمال محرک‌های نانو نقره و نانو دی‌اکسید تیتانیوم با qRT-PCR بررسی شد. نتایج بیانگر افزایش بیان DBOX، SDR، COR و CODM 24 و 48 ساعت پس از تیمار با نانو نقره بود درحالی‌که کاهش بیان SaLAT و T6ODM  در 24 ساعت آغازین مشاهده شد. نانو دی‌اکسید تیتانیوم پس از 48 ساعت منجر به افزایش بیان DBOX، COR و CODM شد. ولی افزایش بیان SDR، SaLAT و T6ODM  تنها پس از 24 ساعت مشاهده شد. بنا بر نتایج به‌دست‌آمده از HPLC نانو نقره به‌صورت بسیار مؤثرتر از نانو دی‌اکسید تیتانیوم پاپاورین، نوسکاپسین و مورفین را به ترتیب 19، 88/1 و 12/3 برابر پس از 48 ساعت در مقایسه با شاهد افزایش داد. بنابراین می‌توان از کشت دروایۀ یاخته‌ای شقایق شرقی به‌عنوان منبعی برای تولید آلکالوئیدهای مهمی مانند مورفین و نوسکاپین استفاده کرد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of nano elicitors on alkaloids production and genes expression in Papaver orientale suspension culture

نویسندگان [English]

  • Safoora Shirvani 1
  • Mohammad Reza Naghavi 2
1 Former M. Sc. Student, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

Plant cell cultures have emerged as potential sources of secondary metabolites. Elicitors play an important role in challenging the plant defense system and altering the secondary metabolite production. Alkaloids are secondary metabolites that have been used as medicine since ancient times. Many Papaver species contain alkaloids such as papaverine, noscapine, morphine and codeine. In this study, we investigated the expression level of genes involved in the biosynthesis of alkaloids in suspension culture of Papaver orientale L. 24 and 48 hours after treatment by nano-Ag and nano-TiO2 with qRT-PCR. qRT-PCR analysis showed that DBOX, SDR, COR and CODM expression were significantly up-regulated at 24 h and 48 h in silver nanoparticles treatment while SaLAT and T6ODM down- regulated. Nano-TiO2 caused up-regulation of DBOX, COR and CODM after 48 h but SDR, SaLAT and T6ODM expression considerably up-regulated at 24 h. The high-performance liquid chromatography analysis indicated the nano-Ag is more effective than nano-TiO2 that increased papaverine, noscapine and morphine respectively 19-fold, 1.88-fold and 3.12-fold in comparison with control 48 h after treatment. This finding indicates that we can use the cell suspension culture of Papaver orientale L. as source of important alkaloids particular morphin and noscapine.

کلیدواژه‌ها [English]

  • Alkaloids
  • Nano elicitors
  • Papaver orientale
  • qRT-PCR
  • suspension culture
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