ارزیابی تحمل به شوری اکوتیپ‌های یونجه با استفاده از شاخص‌های تحمل

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکترای فیزیولوژی گیاهان زراعی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

چکیده

تنش شوری یکی از مهم ترین تنش­های غیر زنده است که همه ساله کشت گیاهان زراعی را در مناطق وسیعی از کشور تحت تأثیر قرار می‌دهد. به منظور بررسی تحمل به شوری اکوتیپ‌های مختلف یونجه آزمایشی به صورت فاکتوریل در قالب طرح بلوک کامل تصادفی با سه تکرار در سال 93 در گلخانه پردیس دانشکده کشاورزی و منابع طبیعی دانشگاه تهران انجام شد. 25 اکوتیپ یونجه در بستر هیدروپونیک (تغذیه با محلول هوگلند) و اعمال سطوح  شوری صفر، 100 و 200 میلی مولار کلرید سدیم همراه با آب آبیاری مورد بررسی قرار گرفتند. نتایج حاصل نشان داد برهمکنش شوری و اکوتیپ‌های مختلف بر وزن خشک اندام هوایی، غلظت سدیم و پتاسیم اندام هوایی و نسبت آنها معنی‌دار شد. جهت تعیین اکوتیپ‌های متحمل و حساس از شاخص‌های تحمل به تنش استفاده شد که نتایج نشان داد عملکرد در شرایط بدون تنش با شاخص­های MP، TOL و STIو در شرایط تنش با MH و STI و YIدارای هم بستگی مثبت و بالایی می­باشد که بر این اساس اکوتیپ‌های 5 و 21 به­عنوان متحمل­ترین و 20 و 8 حساس­ترین معرفی شدند. نتایج تجزیه بای پلات نشان داد، دو مؤلفه اول و دوم 15/87 درصد از تغییرات کل عملکرد را توجیه کردند. لذا، انتخاب بر اساس مؤلفه اول  و دوم به ترتیب موجب گزینش اکوتیپ‌های متحمل و حساس به شوری شد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Evaluation the salt tolerance of alfalfa ecotypes according to tolerance indicator

نویسندگان [English]

  • Samira Ali 1
  • Mojtaba Tavakoli 1
  • Kazem Poustini 2
  • Ahmad Ali Pourbabaei 2
  • Hoshang Alizadeh 2
1 Ph. D. Candidate in Crop Physiology, University College of Agriculture and Natural Resources& Tehran University, Karaj, Iran
2 Professor, University College of Agriculture and Natural Resources& Tehran University, Karaj, Iran
چکیده [English]

Salinity is the most important abiotic stress that influences the crops production in large areas of the country. To evaluate the salt tolerance of different alfalfa ecotypes, an experiment was conducted with three replications in a factorial randomized complete block design in 2014, in College of Agricultural and Natural Resources University of Tehran. 25 alfaalfa ecotypes in hydroponics (Hoagland solution) condition and applied salinity levels 100 and 200 mM sodium chloride with irrigation water were examined. The results indicated that the interaction of salinity and different ecotypes was significant on shoot dry weight, concentration of sodium and potassium. To determine tolerant and sensitive ecotypes indicators of stress were used and the results showed that in non-stress condition yield to MP (mean productivity), TOL (Tolerance), STI (stress tolerance index) indicators and in salt stress yield to MH (Harmonic mean), STI, YI (Yield index) had positively significant correlation. According to this ecotypes 5 and 21 as the most tolerant and ecotype 20 and 8 as more sensitive were introduced. The result of the Biplot analysis showed that the first and second principal components justified 87/15 precent of the whole yield changes. Hence, the selection due to the first and second principal components respectively led to choose the tolerant and sensitive ecotypes.

کلیدواژه‌ها [English]

  • Alfalfa
  • Biplot
  • Stress susceptibility index
  • Stress tolerance index

مراجع

  1. Allakhverdiev, S. I., Sakamoto, A., Nishiyama, Y., Inaba, M. & Murata, N. (2000). Ionic and osmotic effects of NaCl induced inactivation of photosystems I and II in Synechococcus sp. Plant Physiol, 123, 1047-1056.
  2. Asch, F., Dingkuhn, M., Miezan, K. & Doerffling, K. (2000). Leaf K/Na ratio predicts salinity induced yield loss in irrigated rice. Euphytica, 113, 109-118.
  3. Ashraf, M. & McNeilly, T. (2004). Salinity tolerance in Brassica oilseeds. Critical Review of Plant Science, 23, 157-174.
  4. Ashrafi, E., Zahedi, M. & Razmjoo, J. (2014). Co-inoculations of arbuscular mycorrhizal fungi and rhizobia under salinity in alfalfa. Soil Science and Plant Nutrition, 60, 619-629.
  5. Ashrafi, E., Razmjoo, J. & Zahedi, M. (2015). The effect of salt stress on biochemical traits and relation with salt tolerant of alfalfa cultivars in field. Agronomy Journal, 108, 43-56. 
  6. Aydi, S., Sameh, S. & Abdelly, C. (2008). Growth, nitrogen fixation and ion distribuition in Medicago truncatula subjected to salt stress. Plant Soil, 312, 59-67.
  7. Bertrand, A., Dhont, C., Bipfubusa, M., Chalifour, F. P., Drouin, P. & Beauchamp, C. J. (2015). Improving salt stress responses of the symbiosis in alfalfa using salt-tolerant cultivar and rhizobial strain. Applied Soil. Ecology, 87, 108-117.
  8. Brown, J. W. & Hayward, H. E. (1970). Salt tolerance of alfalfa varieties. Agronomy Journal, 63, 18-20.
  9. Cavalanti, F. R., Lima, J. P. M. S., Silva, S. L. F., Viegas, R. A. & Silveira, J. A. G. (2007) Roots and leaves display contrasting Oxidative response during salt stress and recovery in cowpea. Journal of Plant Physiology, 164, 591-600.
  10. Clark, J. M., Depauw, R. M. & Townley-Smith, T. F. (1992). Evaluation of methods for quantification of drought tolerance in wheat. Crop Science, 32, 723-728.
  11. Darvishi, B., Poustini, K. & Tavakolafshary, R. (2005).The reaction of photosynthetic 4 Iranian alfalfa cultivars to salinity stress .Iranian Journal of Agriculture Science, 36(6), 1529-1538. (in Farsi)
  12. Dehdari, A., Rezai, A. & Mirmohamadi Maibody, S. A. M. (2006). Nuclear and cytoplasmic inheritance of salt tolerance in bread wheat plants based on ion contents and biological yield. Iran Agricultural Research, 25(1), 15-26.
  13. Esfandiary, S., Hasanli, A. M., Safari, H. & Farshadfar, M. (2008). Study on drought resistance of five annual medics in Kermanshah province. Iranian Journal of Range Desert Res, 15(2), 283-294.
  14. Fernandez, G. C. J. (1992). Effective selection criteria for assessing plant stress tolerance. In: Proceedings of  the International Symposium on Adaptation of Vegetables and other Food Crops in Temperature and Water Stress, Chapter 25, Taiwan, 13-16 August, pp. 257-270.
  15. Fischer. R. A. & Maurer, R. (1978). Drought resistance in spring wheat cultivars. I. Grain yield response. Australian Journal of Agricultural Research, 29, 897-912.
  16. Fougere, F., Rudulier, D. L. & Streeter, J.G. (1991). Effect of salt stress on amino acid, organic acid, and carbohydrate composition of roots, bactreioids, and cytosol of alfaalfa. Plant Physiology, 96, 1228-1236.
  17. Ghavami, F., Malboobi, M. A., Ghannadha, M. R., Yazdi Samadi, B., Mozaffari. J. & Jafar Aghaei, M. (2004). An evaluation of salt tolerance in Iranian wheat cultivars at germination and seedling stages. Iranian Journal of Agricultural Sciences, 35, 453-464.
  18. Gavuzzi, P., Rizza, F., Palumbo, M., Campaline, R. G., Ricciardi, G. L. & Borghi, B. (1997). Evaluation of field and laboratory predictors of drought and heat tolerance in winter cereals. Canadian Journal of Plant Science, 77, 523-531.
  19. Hajilooi. S. (1998). Genetic study of salt tolerance in wheat diallel method. M.s.c. Thesis. College of Agriculture Tehran University. (in Farsi)
  20. Hashemi Jazi, S. A. M. (1999). Assess the potential impact of salinity on vegetative characteristics of alfalfa varieties.  Iranian Congress of Agronomy, Babolsar.
  21. Hoagland, D. R. & Arnon, D. I. (1950).  The water-culture method for growing plants without soil. California Agricultural Experiment Station Circular, 347.
  22. Jeschke, W. D. & Wolf, O. (1985). Na+-dependent net K+ retranslocation in leaves of Hordeum vulgare, cv. California Mariout and Hordeum distichon, cv. Villa under salt stress. Journal of Plant Physiology, 121, 211-223.
  23. Kanafy Lasku Kalayeh, M., Dehghani, H. & Dourak, J. (2014). Evaluate the response to salt stress in some bread wheat cultivars using stress tolerance indices. Cereal Research, 5(2), 145-157. (in Farsi)
  24. Karimizadeh, R., Dehghani, H. & Dehghanpour, Z. (2006). Determination of genotypic ranks and stability of corn hybrids (Zea mays L.) by nonparametric statistics. Iranian Journal of Agricultural Sciences, 37, 381-388. (in Farsi)
  25. Kattab, H. (2007). Role of glutathione and polyadenylic acid on the oxidative defense systems of two different cultivars of canola seedlings grown under saline condition. Australian Journal of Basic Applied Science, 1, 323-334.
  26. Kingsbury, R. W. & Epstein, E. (1984). Selection for salt-resistant   spring wheat. Crop Science, 24, 310-14.
  27. Khan, M. G., Silberbush, M. & Lips, Sh. (1994). Physiological studies on salinity and nitrogen intraction in alfalfa.1. Biomassproduction and root development. Journal   of Plant –Nutrition, 17(4), 657-668.
  28. Levitt, J. (1980). Responses of Plants to Environmental Stresses. (2nd ed.) Academic Press, New York. pp. 697.
  29. Mass, E.V. & Hoffman, G. J. (1977). Crop salt tolerance-current assessment. Journal of Irrigation and Drainage Engineering, 103, 115-134.
  30. Mirdar Mansuri, Sh., Babaeian Jelodar, N. & Bagheri, N. (2012). Effect of NaCl stress on Iranian rice genotypes in reproductive stage on the base of tolerance indices and screen by biplot method. Journal of Plant Production, 19(1), 68-84. (in Farsi)
  31. Moreno-Gonzalez, J., Crossa, J. & Cornelius, P. L. (2004). Genotype×environment interaction in multi-environment trials using shrinkage factors for AMMI models. Euphytica, 137, 119-127
  32. Munns, R., Schachtman, D. P. & Condon, A. G. (1995). The significance of a two-phase growth response to salinity in wheat and barley. Australian Journal of Plant Physiology, 22, 561-569.
  33. Noble, C. L., Halloran, G. M. & Weast, D. W. (1984). Identification and selection for salt tolerance in Lucerne (Medicago sativa L.). Australian Journal Agriculture of Research, 35, 239-252.
  34. Omielan, J. A., Epstein, E. & Dvorak, F. (1991). Salt tolerance and ionic relations of wheat as affeced by individual chromosomes of salt tolerant Lonphopyrum elongatum. Genome, 34, 961-964.
  35. Owen, C. P. (1992). Plant analysis reference producers for the southern region of the United States. The University of Georgia, PP, 33-45.
  36. Poustini, K. & Siosemardeh, A. (2004). Ion distribution in wheat cultivars in response to salinity stress. Field Crops Research, 85, 125-133.
  37. Qiao, G. R., Zhang, X. G., Jiang, J., Liu, M. Y., Han, X. J. & Yang, H. Q. (2014). Comparative proteomic analysis of responses to salt stress in Chinese willow (Salix Matsudana Koidz). Plant Moecularl Biology Reporter, 32, 814-827.
  38. Rezaian, M. & Ghamari Zare, A. (2000). The effect of saliting on alfalfa ghareh performance, Lain 2129 Australian alfalfa and golpaegani esperes. Articles summary of Iranian Sixth Congress about Sicences and plants improvement. 275. (in Farsi)
  39. Rosielle, A. A. & Hamblin, J. (1981). Theoretical aspect of selection for yield in stress and non - stress environment. Crop Science, 21, 943-946.
  40. Shannon, M. C. & Grieve, C. M. (1999). Tolerance of vegetable crops to salinity. Scientia Horticulturae, 78, 5-38.
  41. Subbarao, G. V. & Johansen, C. (1999). Strategies and scope for improving salinity tolerance in crop plants. (pp. 1069-1087). In Pessaraki, M.Hand book of plant and crop stress. Marcel Dekker Inc. New York. USA.
  42. Yan, W., Hunt, L. A., Sheng, Q. & Szlavnics, Z. (2000). Cultivar evaluation and mega environment investigations based on the GGE biplot. Crop Science, 40, 597-605.

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سابقه مقاله

  • تاریخ دریافت: 17 دی 1395
  • تاریخ بازنگری: 27 تیر 1396
  • تاریخ پذیرش: 27 تیر 1396
  • تاریخ انتشار: 01 شهریور 1396

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