نوع مقاله : مقاله پژوهشی
1 دانشجوی دکتری، گروه زراعت واصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج
2 استاد، گروه زراعت واصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج
3 استادیار، دانشگاه تخصصی فناوریهای نوین آمل
عنوان مقاله [English]
Peppermint known as important medicinal plants that used in the food, medical and pharmaceutical industry. In present study the effect of drought stress at four levels: 100 (as control), 75, 50 and 25% field capacity (FC) were investigated on expression of limonene synthase (lim) and menthol dehydrogenase (mdeh) genes in menthol pathway as well as morpho-physiological responses and performance of peppermint essential oil was evaluated using GC-MS analysis. Results showed that drought stress effect on fresh and dry weight of shoot, leaf area, leaf number, proline content and relative water content (RWC) significantly (P˂0.01) differences. Drought stress caused a significant decrease in the morphological traits also the proline content and relative water content increased and declined respectively. Essential oil yield increased under drought stress condition, although the increase was not significant. The relative expression of lim gene increase at 75 and 50% FC, while showed a significant decreased at 25% FC. The relative expression of mdeh gene decreased at three regime of irrigation compared to control, significantly. In general, drought stress increased oil yield but its quality was declined.