بررسی سطح بیان ژن های lim، mdeh و تغییرات مورفو-فیزیولوژیکی Mentha piperita L. در واکنش به تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه زراعت واصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 استاد، گروه زراعت واصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 استادیار، دانشگاه تخصصی فناوری‌های نوین آمل

چکیده

نعناع فلفلی یکی از گیاهان دارویی با ارزش در جهان به­شمار می­رود که در صنایع غذایی، بهداشتی، داروسازی، و ... کاربرد دارد. در این پژوهش اثر تنش خشکی در چهار سطح: شاهد (100 درصد ظرفیت مزرعه)، 75، 50 و 25 درصد ظرفیت مزرعه بروی بیان ژن­های لیمونن سنتاز (lim) و منتول دهیدروژناز (mdeh) در مسیر بیوسنتز منتول و پاسخ­های مورفولوژیک و فیزیولوژیک نعناع فلفلی و عملکرد و نوع ترکیبات اسانس آن با استفاده از تجزیه GC-MS بررسی شد. براساس نتایج تجزیه واریانس، اثر تنش خشکی بروی صفات وزن تر و خشک اندام هوایی، سطح برگ، تعداد برگ، میزان پرولین و محتوی نسبی آب در سطح احتمال 1 درصد (01/0P˂) معنی­دار بود. تنش خشکی باعث کاهش معنی­داری در صفات مورفولوژیک شد. میزان پرولین و محتوی نسبی آب با افزایش شدت تنش خشکی به­ترتیب افزایش و کاهش یافت. عملکرد اسانس با افزایش تنش خشکی، افزایش یافت هرچند که این افزایش معنی­دار نبود. میزان بیان نسبی ژن لیمونن سنتاز ابتدا افزایش و در سطح 25 درصد ظرفیت مزرعه کاهش معنی­داری یافت همچنین میزان بیان نسبی ژن منتول دهیدروژناز در سطوح مختلف تنش خشکی کاهش نشان داد. به­طور کلی تنش خشکی باعث افزایش عملکرد اسانس ولی کاهش کیفیت آن شد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigation of lim, medh gene expression level and morpho-physiological changes of Mentha piperiat L. in response to drought

نویسندگان [English]

  • Yousef Rahimi 1
  • Alireza Taleei 2
  • Mojtaba Ranjbar 3
1 Ph.D. Candidate, Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Assistant Professor, Amol University of Special Modern Technologies, Amol, Iran
چکیده [English]

Peppermint known as important medicinal plants that used in the food, medical and pharmaceutical industry. In present study the effect of drought stress at four levels: 100 (as control), 75, 50 and 25% field capacity (FC) were investigated on expression of limonene synthase (lim) and menthol dehydrogenase (mdeh) genes in menthol pathway as well as morpho-physiological responses and performance of peppermint essential oil was evaluated using GC-MS analysis. Results showed that drought stress effect on fresh and dry weight of shoot, leaf area, leaf number, proline content and relative water content (RWC) significantly (P˂0.01) differences. Drought stress caused a significant decrease in the morphological traits also the proline content and relative water content increased and declined respectively. Essential oil yield increased under drought stress condition, although the increase was not significant. The relative expression of lim gene increase at 75 and 50­% FC, while showed a significant decreased at 25% FC. The relative expression of mdeh gene decreased at three regime of irrigation compared to control, significantly. In general, drought stress increased oil yield but its quality was declined.

کلیدواژه‌ها [English]

  • Drought stress
  • lim gene
  • mdeh gene
  • morpho-physiological traits
  • Peppermint

مراجع

  1. Abdalla, M. M. & El-Khoshiban, N. H. (2007). The influence of water stress on growth, relative water content, photosynthetic pigments, some metabolic and hormonal contents of two Triticium aestivum cultivars. Journal of Applied Sciences Research, 3(12), 2062-2074.
  2. Afkar, S., Karimzadeh, G., Jalali-Javaran, M., Sharifi, M. & Behmanesh, M. (2013). Influence of methyl jasmonate on menthol production and gene expression in peppermint (Mentha X piperita L.). Journal of medicinal plants and by-products, 1, 75-82.
  3. Alishah, H. M., Heidari, R., Hassani, A. & Dizaji, A. A. (2006). Effect of water stress on some morphological and biochemical characteristics of purple basil (Ocimum basilicum). Biological Sciences, 6(4), 763-767.
  4. Alishah, H. M., Heidari, R., Hassani, A. & Dizaji, A. A. (2006). Effect of water stress on some morphological and biochemical characteristics of purple basil (Ocimum basilicum). Biological Sciences, 6(4), 763-767.
  5. Alonso, W. R., Rajaonarivony, J. I., Gershenzon, J. & Croteau, R. (1992). Purification of 4S-limonene synthase, a monoterpene cyclase from the glandular trichomes of peppermint (Mentha x piperita) and spearmint (Mentha spicata). Journal of Biological Chemistry, 267(11), 7582-7587.
  6. Aziz EA Hendawi, T., Azza, EED. & Omer, EA. (2008). Effect of Soil Type and Irrigation Intervals on Plant Growth, Essential Oil and Constituents of Thymus vulgaris Plant. American-Eurasian Journal of Agriculture and Environmental Science, 4(4), 443-450.
  7. Aziz, E. E., Al-Amier, H. & Craker, L. E. (2008). Influence of salt stress on growth and essential oil production in peppermint, pennyroyal, and apple mint. Journal of herbs, spices & medicinal plants, 14(1-2), 77-87.
  8. Baher, F., Mirza, M., Ghorbanli, M. & Bagher Rezaii, M. (2002). The influence of water stress on plant height, herbal and essential oil yield and composition in Satureja hortensis L. Flavour and Fragrance Journal, 17(4), 275-277.
  9. Baher, Z. F., Mirza, M., Ghorbanli, M. & Bagher Rezaii, M. (2002). The influence of water stress on plant height, herbal and essential oil yield and composition in Satureja hortensis L. Flavour and Fragrance Journal, 17(4), 275-277.
  10. Baser, K.H.C. (1997). Industrial utilization of medicinal and aromatic plants. Acta Horticulturae, 503, 177-192.
  11. Bates, L. S., Waldren, R. P. & Teare, I. D. (1973). Rapid determination of proline for water stress studies. Plant and Soil, 39, 205-208.
  12. Bin Zakaria, M. & Mohd, M. A. (2010). Traditional Malay medicinal plants. ITBM.
  13. Bose, S. K., Yadav, R. K., Mishra, S., Sangwan, R. S., Singh, A. K., Mishra, B. & Sangwan, N. S. (2013). Effect of gibberellic acid and calliterpenone on plant growth attributes trichomes, essential oil biosynthesis and pathway gene expression in differential manner in Mentha arvensis L. Plant Physiology and Biochemistry, 66, 150-158.
  14. Croteau, R. B., Davis, E. M., Ringer, K. L. & Wildung, M. R. (2005). (−)-Menthol biosynthesis and molecular genetics. Naturwissenschaften, 92(12), 562-577.
  15. Croteau, R. & Gershenzon, J. (1994). Genetic control of monoterpene biosynthesis in mints (Mentha: Lamiaceae). In Genetic engineering of plant secondary metabolism (pp. 193-229). Springer US.
  16. Davis, E. M. & Croteau, R. (2000). Cyclization enzymes in the biosynthesis of monoterpenes, sesquiterpenes, and diterpenes. In Biosynthesis (pp. 53-95). Springer Berlin Heidelberg.
  17. Descalzo, AM. & Sancho, AM. (2008). A review of natural antioxidants and their effects on oxidative status, odor and quality of fresh beef produced in Argentine. Meat Science, 79, 423-436.
  18. Fan, J., Ding, X. & Gu, W. (2007). Radical-scavenging proanthocyanidins from sea buckthorn seed. Food Chemistry, 102, 168-177.
  19. Farahani, HA., Valadabadi, SA., Daneshian, J. & Khalvati, MA. (2009). Evaluation changing of essential oil of balm (Melissa officinalis L.) under water deficit stress conditions. Journal of Medicinal Plants Research, 3(5): 329-333.
  20. Fatima, S., Farooqi, A. A. & Sharma, S. (2002). Physiological and metabolic responses of different genotypes of Cymbopogon martinii and C. winterianus to water stress. Plant Growth Regulation, 37(2), 143-149.
  21. Gedif, T. & Hahn, H. J. (2002). Herbalists in Addis Ababa and Butajira, Central Ethiopia: Mode of service delivery and traditional pharmaceutical practice. Ethiopian Journal of Health Development, 16(2), 183-189.
  22. Gershenzon, J. (1984). Changes in the levels of plant secondary metabolites under water and nutrient stress. In Phytochemical adaptations to stress (pp. 273-320). Springer USA.
  23. Gómez-Galera, S., Pelacho, A. M., Gené, A., Capell, T. & Christou, P. (2007). The genetic manipulation of medicinal and aromatic plants. Plant Cell Reports, 26, 1689-715.
  24. Goren, N., Demirci, B. & Baser, K. H. C. (2001). Composition of the essential oils of anacetum spp. from Turkey. Flavour and fragrance journal, 16: 191-194.
  25. Hassanpour, H., Khavari-Nejad, R. A., Niknam, V., Najafi, F. & Razavi, K. (2012). Effects of penconazole and water deficit stress on physiological and antioxidative responses in pennyroyal (Mentha pulegium L.). Acta Physiologiae Plantarum, 34(4), 1537-1549.
  26. Irrigoyen, J. H., Emerich, D. W. & Sanchez, D. M. (1992). Water stress induced changes in concentrations of proline and total soluble sugars in modulated alfalfa (Medicago sativa) plants. Physiologia Plantarum, 84, 55-60.
  27. Jaleel, C. A., Manivannan, P. A., Wahid, A., Farooq, M., Al-Juburi, H. J., Somasundaram, R. A. & Panneerselvam, R. (2009). Drought stress in plants: a review on morphological characteristics and pigments composition. International Journal of Agriculture & Biology, 11(1), 100-5.
  28. Kang, Y. M., Park, D. J., Lee, D. G., Song, H. J., Kang, S. M., MIN, J., ... & Choi, M. S. (2015). Over expression of IPP isomerase and limonene synthase enzymes in Mentha spicata and their influence on the terpenoid metabolism. Romanian Biotechnological Letters, 20(2), 10358-10368.
  29. Khalil, S. E., Nahed, G., Azizi, A. & Bedour, L. A. H. (2010). Effect of water stress and ascorbic acid on some morphological and biochemical composition of Ocimum basilicum plant. Journal of American Science, 6, 33-44.
  30. Khorasaninejad, S., Mousavi, A., Soltanloo, H., Hemmati, K. & Khalighi, A. (2011). The effect of drought stress on growth parameters, essential oil yield and constituent of Peppermint (Mentha piperita L.). Journal of Medicinal Plants Research, 5(22), 5360-5365.
  31. Kjonaas, R. & Croteau, R. (1983). Demonstration that limonene is the first cyclic intermediate in the biosynthesis of oxygenated p-menthane monoterpenes in Mentha piperita and other Mentha species. Archives of biochemistry and biophysics, 220(1), 79-89.
  32. Krasnyanski, S., May, R. A., Loskutov, A., Ball, T. M. & Sink, K. C. (1999). Transformation of the limonene synthase gene into peppermint (Mentha piperita L.) and preliminary studies on the essential oil profiles of single transgenic plants. Theoretical and Applied Genetics, 99(3-4), 676-682.
  33. Lange, B. M., Mahmoud, S. S., Wildung, M. R., Turner, G. W., Davis, E. M., Lange, I., ... & Croteau, R. B. (2011). Improving peppermint essential oil yield and composition by metabolic engineering. Proceedings of the National Academy of Sciences, 108(41), 16944-16949.
  34. Liu, H., Qiu, N., Ding, H. & Yao, R. (2008). Polyphenols contents and antioxidant capacity of 68 Chinese herbals suitable for medicinal or food uses. Food Reseach International, 41(4), 363-370.
  35. Maffei, M., Camusso, W. & Sacco, S. (2001). Effect of Mentha piperita essential oil and monoterpenes on cucumber root membrane potential. Phytochemistry, 58(5), 703-707.
  36. Muzemil, A. (2008). Determination of artemisinin and essential oil contents of Artemisia annua L. grown in Ethiopia and in vivo antimalarial activity of its crude extracts against Plasmodium berghei in mice (Doctoral dissertation, Department of Pharmaceutical Chemistry, School of Pharmacy, Addis Ababa University).
  37. Ozturk, A. H. M. E. T., Unlukara, A., Ipek, A. R. İ. F. & Gurbuz, B. İ. L. A. L. (2004). Effects of salt stress and water deficit on plant growth and essential oil content of lemon balm (Melissa officinalis L.). Pakistan Journal of Botany, 36(4), 787-792.
  38. Petropoulos, S. A., Daferera, D., Polissiou, M. G. & Passam, H. C. (2008). The effect of water deficit stress on the growth, yield and composition of essential oils of parsley. Scientia Horticulturae, 115(4), 393-397.
  39. Pfaffl, M. W., Horgan, G. W. & Dempfle, L. (2002). Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research, 30(9), e36-e36.
  40. Pirzad, A., Shakiba, M. R., Zehtab-Salmasi, S., Mohammadi, S. A., Darvishzadeh, R. & Samadi, A. (2011). Effect of water stress on leaf relative water content, chlorophyll, proline and soluble carbohydrates in Matricaria chamomilla L. Journal of Medicinal Plants Research, 5(12), 2483-2488.
  41. Pirzad, A., Shakiba, M. R., Zehtab-Salmasi, S., Mohammadi, S. A., Darvishzadeh, R. & Samadi, A. (2011). Effect of water stress on leaf relative water content, chlorophyll, proline and soluble carbohydrates in Matricaria chamomilla L. Journal of Medicinal Plants Research, 5(12), 2483-2488.
  42. Rai, V., Vajpayee, P., Singh, S. N. & Mehrotra, S. (2004). Effect of chromium accumulation on photosynthetic pigments, oxidative stress defense system, nitrate reduction, proline level and eugenol content of Ocimum tenuiflorum L. Plant science, 167(5), 1159-1169.
  43. Razmjoo, K. H., Heydarizadeh, P. & Sabzalian, M. (2008). Effect of salinity and drought stresses on growth parameters and essential oil content of Matricaria chamomile. International Journal of Agriculture & Biology, 10(4), 451-454.
  44. Rebey, I. B., Jabri-Karoui, I., Hamrouni-Sellami, I., Bourgou, S., Limam, F. & Marzouk, B. (2012). Effect of drought on the biochemical composition and antioxidant activities of cumin (Cuminum cyminum L.) seeds. Industrial Crops and Products, 36(1), 238-245.
  45. Rohdich, F., Bacher, A. & Eisenreich, W. (2005). Isoprenoid biosynthetic pathways as anti-infective drug targets. Biochemical Society Transactions, 33, 785-791.
  46. Rohmer, M. (2003). Mevalonate-independent methylerythritol phosphate pathway for isoprenoid biosynthesis. Elucidation and distribution. Pure and Applied Chemistry, 75, 375-387.
  47. Sangwan, N. S., Farooqi, A. H. A., Shabih, F. & Sangwan, R. S. (2001). Regulation of essential oil production in plants. Plant growth regulation, 34(1), 3-21.
  48. Sankar, B., Jaleel, C. A., Manivannan, P., Kishorekumar, A., Somasundaram, R. & Panneerselvam, R. (2007). Drought-induced biochemical modifications and proline metabolism in Abelmoschus esculentus (L.) Moench. Acta Botanica Croatica, 66(1), 43-56.
  49. Sanchez, S. R. (1998). Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress. Field crops research, 59, 225-235.
  50. Sadeghian, M., Hakimi, M. H. & Sodaeizadeh, H. (2015). Effect of drought stress on some physiological and morphological characteristics of Hymenocrater yazdianus. Journal of Biological and Environmental Sciences, 7, 110-119.
  51. Sequera-Mutiozabal, M., Tiburcio, A. F. & Alcázar, R. (2016). Drought Stress Tolerance in Relation to Polyamine Metabolism in Plants. In: Drought Stress Tolerance in Plants, 1, 267-286. Springer International Publishing.
  52. Serraj, R. & Sinclair, T. R. (2002). Osmolyte accumulation: can it really help increase crop yield under drought conditions? Plant, Cell & Environment, 25(2), 333-341.
  53. Siddique, M. R. B., Hamid, A. & Islam, M. S. (2000). Drought stress effects on water relations of wheat. Botanical Bulletin of Academia Sinica, 41.
  54. Sustrikova, A. & Salamon, I. (2004). Essential oil of peppermint (Mentha× piperita L.) from fields in Eastern Slovakia. Horticutural Science, 31, 31-6.
  55. Todaka, D., Shinozaki, K. & Yamaguchi-Shinozaki, K. (2015). Recent advances in the dissection of drought-stress regulatory networks and strategies for development of drought-tolerant transgenic rice plants. Frontiers in Plant Science, 6, 84.
  56. Tateo, F. & Riva, G. (1991). Influence of the drying process on the quality of essential oils in Artemisia absinthium. Mitteilungen aus dem Gebiete der Lebensmitteluntersuchung und Hygiene, 82, 607-614.
  57. Yadegarinia, D., Gachkar, L., Rezaei, M. B., Taghizadeh, M., Astaneh, S. A. & Rasooli, I. (2006). Biochemical activities of Iranian Mentha piperita L. and Myrtus communis L. essential oils. Phytochemistry, 67(12), 1249-1255.

فایل ها

سابقه مقاله

  • تاریخ دریافت: 28 مهر 1395
  • تاریخ بازنگری: 14 دی 1395
  • تاریخ پذیرش: 02 اسفند 1395
  • تاریخ انتشار: 01 شهریور 1396

هم رسانی

ارجاع به این مقاله

آمار