بررسی سطح بیان ژن های lim، mdeh و تغییرات مورفو-فیزیولوژیکی Mentha piperita L. در واکنش به تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه زراعت واصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 استاد، گروه زراعت واصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 استادیار، دانشگاه تخصصی فناوری‌های نوین آمل

چکیده

نعناع فلفلی یکی از گیاهان دارویی با ارزش در جهان به­شمار می­رود که در صنایع غذایی، بهداشتی، داروسازی، و ... کاربرد دارد. در این پژوهش اثر تنش خشکی در چهار سطح: شاهد (100 درصد ظرفیت مزرعه)، 75، 50 و 25 درصد ظرفیت مزرعه بروی بیان ژن­های لیمونن سنتاز (lim) و منتول دهیدروژناز (mdeh) در مسیر بیوسنتز منتول و پاسخ­های مورفولوژیک و فیزیولوژیک نعناع فلفلی و عملکرد و نوع ترکیبات اسانس آن با استفاده از تجزیه GC-MS بررسی شد. براساس نتایج تجزیه واریانس، اثر تنش خشکی بروی صفات وزن تر و خشک اندام هوایی، سطح برگ، تعداد برگ، میزان پرولین و محتوی نسبی آب در سطح احتمال 1 درصد (01/0) معنی­دار بود. تنش خشکی باعث کاهش معنی­داری در صفات مورفولوژیک شد. میزان پرولین و محتوی نسبی آب با افزایش شدت تنش خشکی به­ترتیب افزایش و کاهش یافت. عملکرد اسانس با افزایش تنش خشکی، افزایش یافت هرچند که این افزایش معنی­دار نبود. میزان بیان نسبی ژن لیمونن سنتاز ابتدا افزایش و در سطح 25 درصد ظرفیت مزرعه کاهش معنی­داری یافت همچنین میزان بیان نسبی ژن منتول دهیدروژناز در سطوح مختلف تنش خشکی کاهش نشان داد. به­طور کلی تنش خشکی باعث افزایش عملکرد اسانس ولی کاهش کیفیت آن شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Investigation of lim, medh gene expression level and morpho-physiological changes of Mentha piperiat L. in response to drought

نویسندگان [English]

  • Yousef Rahimi 1
  • Alireza Taleei 2
  • Mojtaba Ranjbar 3
1 Ph.D. Candidate, Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Assistant Professor, Amol University of Special Modern Technologies, Amol, Iran
چکیده [English]

Peppermint known as important medicinal plants that used in the food, medical and pharmaceutical industry. In present study the effect of drought stress at four levels: 100 (as control), 75, 50 and 25% field capacity (FC) were investigated on expression of limonene synthase (lim) and menthol dehydrogenase (mdeh) genes in menthol pathway as well as morpho-physiological responses and performance of peppermint essential oil was evaluated using GC-MS analysis. Results showed that drought stress effect on fresh and dry weight of shoot, leaf area, leaf number, proline content and relative water content (RWC) significantly (P˂0.01) differences. Drought stress caused a significant decrease in the morphological traits also the proline content and relative water content increased and declined respectively. Essential oil yield increased under drought stress condition, although the increase was not significant. The relative expression of lim gene increase at 75 and 50­% FC, while showed a significant decreased at 25% FC. The relative expression of mdeh gene decreased at three regime of irrigation compared to control, significantly. In general, drought stress increased oil yield but its quality was declined.

کلیدواژه‌ها [English]

  • Drought stress
  • lim gene
  • mdeh gene
  • morpho-physiological traits
  • Peppermint
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