جداسازی و شناسایی باکتری‌های محرک رشد از ریشه‌گاه آویشن دنایی و بررسی توانایی آن‌ها در بهبود جوانه‌زنی بذر

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموختۀ دکتری زراعت، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 استادیار، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 استادیار پژوهشکدۀ گیاهان و مواد اولیۀ دارویی، دانشگاه شهید بهشتی

4 استاد، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

5 استادیار گروه خاکشناسی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران

چکیده

آویشن دنایی (Thymus daenensis subsp. Daenensis) گیاه دارویی بومی ایران بوده و ازنظر فنول‌های مونوترپن ]به‌ویژه تیمول (thymol) و کارواکرول (carvacrol)[ غنی است. هدف از این تحقیق جداسازی و شناسایی ریزوباکتری‌های بهبوددهندۀ رشد گیاه (PGPRs) از ریشه‌گاه (ریزوسفر) آویشن دنایی است. غربالگری سویه‌های PGPR با استفاده از آزمون‌های فیزیولوژیکی مانند توانایی تولید اکسین، توانایی تولید ترکیبات آهن‌بر (سیدروفور) و حل‌کنندگی فسفات و همچنین آزمون‌های جوانه‌زنی و قوۀ نامیِۀ بذر آویشن دنایی انجام شد. 21 جدایۀ باکتری از نمونۀ ریشه‌گاه آویشن دنایی که از رویشگاه طبیعی گردآوری شده بودند، جداسازی شد و تأثیر تلقیح آن‌ها روی ویژگی‌های جوانه‌زنی آویشن دنایی در یک تحقیق آزمایشگاهی در قالب طرح بلوک کامل تصادفی با سه تکرار بررسی شد. چهار جدایه به‌عنوان جدایه‌های PGPRشناسایی شدند. جدایه‌های TDE3،  TDE16 و TDE20 توانایی تولید IAA، ترکیبات سیدروفور و حل‌کنندگی فسفات نامحلول را داشتند. جدایۀ TDE4 (Achromobacter spiritinus) تنها جدایه‌ای بود که سرعت جوانه‌زنی و شاخص بنیة (ویگور) بذر را به‌طور معنی‌دار افزایش داد. چهار جدایۀ یادشده این قابلیت (پتانسیل) را دارند تا به‌عنوان بهبوددهندۀ رشد گیاه (PGPR)، در کشت آویشن دنایی استفاده شوند.

کلیدواژه‌ها

عنوان مقاله [English]

Isolation and characterization of growth-promoting bacteria from rhizosphere of Thymus danenensis and study of their potential for enhancement of seed germination

نویسندگان [English]

  • Amir Hossein Agha Ahmadi 1
  • Hossein Moghadam 2
  • Farzad Najafi 3
  • Daryoush Mazaheri 4
  • Ahmad Ali Pourbabaee 5
1 Former Ph. D. Student, Department of Agronomy and Plant Breeding, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Assistant Professor, Department of Agronomy and Plant Breeding, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Assistant Professor, Medicinal Plants & Drug Research Institute, Shahid Beheshti University, Tehran, Iran
4 Professor, Department of Agronomy and Plant Breeding, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
5 Assistant Professor, Soil Science Department, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

The Iranian Thymus daenensis subsp. daenensis is rich in monoterpene phenols (especially thymol and carvacrol). This research was conducted to isolate and characterize the plant growth promoting rhizobacteria (PGPRs) from rhizosphere of T. daenensis. PGPRs were screened based on some physiological traits including IAA production, phosphate solubilization, and siderophore compounds production abilities, and also seed germination and vigor tests. Twenty one rhizobacteria were isolated from the rhizosphere samples of T. daenensis which grow widely in Iran, and their effect on seed germination properties was studied in a completely randomized block design with three replications. We could characterize four isolates for PGPRs activity based on physiological and germination assays. TDE3, TDE16 and TDE20 isolates had the phosphate solubilization and siderophores production abilities. TDE4 (Achromobacter spiritinus) was the only isolated bacteria which significantly improved the SGR and VI of T. daenensis seeds. These four isolates have the potential to be used as PGPRs in cultivation of T. daenensis.

کلیدواژه‌ها [English]

  • IAA production
  • PGPRs
  • Seed germination
  • seedling vigor
  • Thymus daenensis

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سابقه مقاله

  • تاریخ دریافت: 29 مرداد 1393
  • تاریخ بازنگری: 27 اسفند 1393
  • تاریخ پذیرش: 19 آبان 1394
  • تاریخ انتشار: 01 خرداد 1395

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