تأثیر پیش‌تیمار بذر با آب بر جوانه‌زنی، رشد و فعالیت آنزیم‌های پاداکسندۀ گیاهچه‌های گاوزبان اروپایی (Borago officinalis L.) در شرایط تنش کادمیوم

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، استادیار و دانشیار، گروه زراعت و اصلاح نباتات، دانشگاه محقق اردبیلی، اردبیل

2 استادیار، گروه زراعت و اصلاح نباتات، دانشگاه محقق اردبیلی، اردبیل

3 دانشیار، گروه زراعت و اصلاح نباتات، دانشگاه محقق اردبیلی، اردبیل

چکیده

کادمیوم یکی از فلزهای سنگین و از جمله آلاینده‌های مهم محیط‌زیست است که جوانه‌زنی و رشد و نمو گیاهان را مهار می‌کند، لذا در این شرایط استفاده از روش‎هایی که جوانه‎زنی گیاه را تحریک کند ضروری به نظر می‎رسد. در این تحقیق تأثیر پیش‌تیمار بذر با آب (هیدروپرایمینگ) بر شاخص‌های جوانه‌زنی و رشد گیاهچه‌های گیاه دارویی گاوزبان اروپایی (Borago officinalis L.) در شرایط تنش کادمیوم به­صورت فاکتوریل در قالب طرح کامل تصادفی با سه تکرار در سال 1394 در دانشگاه محقق اردبیلی بررسی شد. عامل‌های آزمایشی شامل تنش کادمیوم در چهار سطح (1S، 2S، 3S و 4S: به ترتیب 0، 10، 50 و 100 میلی­گرم کادمیوم در لیتر) و پیش‌تیمار بذر با آب در چهار سطح (0 (شاهد)، 12، 24 و 48 ساعت) بود. نتایج نشان داد، با افزایش شدت تنش، میانگین درصد و سرعت جوانه‌زنی، وزن خشک و طول گیاهچه، شاخص­‌های طولی و وزنی توان و فعالیت آنزیم­های کاتالاز و پراکسیداز کاهش یافت. در شرایط بدون تنش، درصد و سرعت جوانه‌زنی و وزن خشک گیاهچه­های ناشی از بذرهای پیش‌تیمار‌شده به‌طور معنی‌داری بیشتر از بذرهای شاهد بود. پیش‌تیمار بذر با آب شاخص‌های جوانه‌زنی و رشد گیاهچه‌های گاوزبان اروپایی در شرایط تنش کادمیوم را به‌طور معنی‌داری افزایش داد. به‌طوری‌که، پیش‌تیمار بذر با آب به مدت 48 ساعت سبب افزایش معنی‌دار و دو برابری در سرعت جوانه‌زنی بذرها، 5/4 برابری شاخص وزنی گیاهچه‌ها، 5/3 برابری فعالیت آنزیم‌ کاتالاز و 5/2 برابری فعالیت آنزیم‌ پراکسیداز نسبت به شاهد شد. در مجموع، پیش‌تیمار بذر با آب بذر به مدت 48 ساعت بیشترین اثر مثبت را بر جوانه‌زنی و رشد گیاهچه‌های گاوزبان اروپایی داشت.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of hydropriming on germination, growth and antioxidant enzymes Activity of Borage (Borago officinalis L.) seedling under cadmium stress

نویسندگان [English]

  • Fatemeh Mahmoudi 1
  • Parisa Sheikhzadeh Mosaddegh 2
  • Naser Zare 3
  • Behrouz Esmaielpour 3
1 Former MSc. Student, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran
2 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran
3 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran
چکیده [English]

Cadmium is one of the heavy metals and the important environmental pollutants that inhibits seed germination and plant growth and development. So, in these conditions, the use of methods for stimulation of seed germination is necessary. In this research, the effects of hydropriming on germination and growth indice of borage seedlings under cadmium stress were investigated in a factorial experiment based on completely randomized design with three replications at the University of Mohaghegh Ardabili in 2015. The Experimental treatments were cadmium stress with four levels (S1, S2, S3 and S4; zero (control), 10, 50 and 100 mg.lit-1 ) and hydropriming with four levels (12, 24 and 48 hours). The results showed that the percentage and rate of germination, length and seedling dry weight, vigor indice and catalase and peroxidase enzymes activity were decreased with increasing stress level. In non stress condition, the percentage and rate of seed germination and seedlings dry weight of seedling derive from primed seeds were significantly higher than those of control seeds. Hydropriming significantly increased the seed germination and seedling growth indices of borage under cadmium stress conditions. 48 hours hydropriming under cadmium stress conditions, caused significant and 2-fold increase in the germination rate, 4.5-fold in the seedling weight index, 3.5 and 2.5-fold in the activity of catalase and peroxidase enzymes, respectively, as compared to control. In total,. 48 hours hydropriming was the most positive effects on seed germination and growth of borage seedling.

کلیدواژه‌ها [English]

  • catalase enzyme
  • Heavy metals
  • Medicinal plant
  • Peroxidase enzyme
  • Seedling growth

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سابقه مقاله

  • تاریخ دریافت: 19 اردیبهشت 1395
  • تاریخ بازنگری: 12 مرداد 1395
  • تاریخ پذیرش: 17 مهر 1395
  • تاریخ انتشار: 01 خرداد 1396

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