تأثیر تنش خشکی بر جوانه‌زنی و محتوای رنگدانه‌های نورساختی در ژنوتیپ‌های لوبیای معمولی (Phaseolus vulgaris L.)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 دانشیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

چکیده

به‌منظور بررسی تأثیر تنش خشکی بر جوانه­زنی بذر، 16 ژنوتیپ لوبیای معمولی در سطوح مختلف تنش اسمزی شاهد، 4-، 8- و 12- بار پلی‌اتیلن گلیکول 6000 در یک آزمایش فاکتوریل در قالب طرح کامل تصادفی با سه تکرار بررسی شد. تنش خشکی از روز اول کشت بذر در پتری دیش اعمال و نمونه­گیری 9 روز پس از اعمال تنش از ریشه­چه و ساقه­چه انجام شد. نتایج نشان داد، تنش خشکی باعث کاهش معنی­دار درصد و سرعت جوانه­زنی، طول ریشه­چه و ساقه­چه، وزن تر و خشک ریشه­چه و ساقه­چه می­شود. به‌منظور ارزیابی تأثیر فیزیولوژی مقاومت به تنش خشکی سه ژنوتیپ K-S-31167، GE-288 و Naz حاصل از غربالگری آزمایش جوانه­زنی در سطوح تنش 75، 50 و 25 درصد ظرفیت زراعی به­همراه شاهد در مرحلۀ گیاهچه­ای بررسی شد. تنش خشکی پنج هفته پس از کاشت اعمال و ده روز پس از اعمال تنش نمونه­گیری از سه­ برگچۀ دوم انجام شد. نتایج نشان داد که تنش خشکی باعث تغییر در محتوای رنگدانه­های نورساختی (فتوسنتزی) و محتوای نسبی آب می­شود. ژنوتیپ متحمل K-S-31167 نسبت به دو ژنوتیپ به نسبت متحمل GE-288 و حساس Naz رنگدانه­های نورساختی و محتوای نسبی آب بیشتر داشت. که نشان‌دهندۀ پایداری غشایی بالاتر این ژنوتیپ در شرایط تنش است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effects of drought stress on germination and photosynthetic pigments content of common bean (Phaseolus vulgaris L.) genotypes

نویسندگان [English]

  • Elham Soltani 1
  • Abdol Hadi Hosseinzadeh 2
  • Alireza Abbasi 2
1 Former M. Sc. Student, Department of Agronomy and Plant Breeding, Agricultural Science and Engineering College, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Associate Professor, Department of Agronomy and Plant Breeding, Agricultural Science and Engineering College, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

To evaluate the effects of drought stress on seed characteristics, seeds of the tolerant and sensitive genotyps of common bean were placed in petri dishes containing polyethylene glycol 6000 with concentration equal to -4, -8 and -12 bar, according to CRD Design with three replication using factorial arrengment, Nine day later, radicle and caulicle length were measured. The result showed that drought stress decreased germination speed and percentage, radicle and caulicle length, radicle fresh and dry weight, caulicle fresh and dry weight. To study the physiological processes, three genotypes including K-S-31167, GE-288 and Naz witch were respectivly tolerant, relatively tolerant and sensitive to drought stress were selected from first expriment and were subjected to 4 level of drought stress based on 25, 50, 75 and 100 percent of field capacity using CRD design with three replication using factorial arrengment, Droght stress was applied five weeks after planting and lasted for 10 days. Second terifoliate were used for sampling. The results indicated significant changes in the photosynthetic pigments content (PPC) and relative water content (RWC). Tolerant genotype (K-S-31167) had greater PPC and RWC compare to relatively tolerant genotype (GE-288) and sensitive genotype (Naz). This indicate that resistant genotype had higher membrane stability during stress.

کلیدواژه‌ها [English]

  • common bean
  • Drought
  • germination
  • photosynthetic pigments
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