ارزیابی فعالیت آنزیم گایکول پراکسیداز در ژنوتیپ‌های کلزا در شرایط تنش و بدون تنش اسمزی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، بیوتکنولوژی کشاورزی- گیاهی، دانشکدۀ علوم کشاورزی، دانشگاه گیلان

2 دانشیار، بیوتکنولوژی کشاورزی- گیاهی، دانشکدۀ علوم کشاورزی، دانشگاه گیلان

3 استادیار، بیوتکنولوژی کشاورزی- گیاهی، دانشکدۀ علوم کشاورزی، دانشگاه گیلان

چکیده

گونه‌های اکسیژن فعال به‌طورمعمول بی‌درنگ به‌وسیلۀ مولکول‌های پاداکسنده (آنتی‌اکسیدان)‌ که در یاخته حضور دارند پاک‌سازی می‌شوند. پراکسیدازها یکی از آنزیم‌های مهم پاداکسنده هستند که می‌توانند با استفاده از پراکسید هیدروژن، بسیاری از بستره (سوبسترا)های آلی و غیر آلی را اکسید کنند. در این تحقیق فعالیت آنزیم پراکسیداز در عصاره‌های استخراج‌شده از کلزا در غلظت‌های مختلف گایکول بررسی شد. نمونه­برداری همزمان از گیاهچه‌های کلزای در شرایط تیمار تنش و بدون تنش اسمزی، در مرحلۀ 4 تا 6 برگی و در چهار زمان 4، 8، 12 و 24 ساعت پس از اعمال تنش اسمزی، انجام شد. نتایج نشان داد که میزان فعالیت آنزیم پراکسیداز در دو ژنوتیپ مقاوم (SLM046) و حساس (Hyola308) در ساعت‌های مختلف ارزیابی‌شده در شرایط بدون تنش متفاوت بوده و در ژنوتیپ SLM046 بیشترین فعالیت در 12 ساعت پس از اعمال تنش مشاهده شد. مقایسۀ دو ژنوتیپ‌ Hyola308 و SLM046 در شرایط تنش نشان داد که فعالیت آنزیم پراکسیداز در ساعت‌های‌ 4، 8 و 12 پس از اعمال تنش به­ترتیب در SLM046 بیشتر از Hyola308 است. در 24 ساعت پس از نمونه‌برداری بیشترین میزان فعالیت مربوط به Hyola308 شاهد در غلظت 60 میلی‌مولار و کمترین میزان فعالیت مربوط به ژنوتیپ SLM046 تنش دیده بود. نتایج نشان داد، بالا بودن فعالیت آنزیم پراکسیداز در شرایط تنش، در ژنوتیپ SLM046 در مقایسه با Hyola308، سبب می‌شود تجمع H2O2در SLM046 کمتر از Hyola308 باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigation of guaiacol peroxidase (GPx) activity in two canola genotype under non-stress and osmotic conditions

نویسندگان [English]

  • Mohammad Mohsenzadeh Golfazane 1
  • Habibollah Samizadeh Lahiji 2
  • Hassan Hassani kumleh 3
1 Ph. D. Candidate, Associate Professor and Assistant Professor, Faculty of Agriculture, University of Guilan, Iran
2 Associate Professor, Faculty of Agriculture, University of Guilan, Iran
3 Assistant Professor, Faculty of Agriculture, University of Guilan, Iran
چکیده [English]

Reactive oxygen species in the cell are rapidly removed by antioxidant molecules. Peroxidases are one of the important antioxidant enzymes that can oxidize many organic and inorganic substrates using hydrogen peroxide. In this study, peroxidase enzyme activities of canola extracts were evaluated in different guaiacol concentrations. Simultaneous sampling of rape seedling under non-stress and osmotic stress condition was conducted in 4 - 6 leaf stage at 4, 8, 12 and 24 hours after osmotic stress. The results showed that the GPx activity in non-stress condition was different in both Hyola308 (susceptible to drought) and SLM046 (tolerant to drought) varieties at different times of exposure to stress, and the highest activity of enzyme in SLM046 were observed at 12 hours after stress. Comparison of the two genotypes under stress condition showed that GPx activity in SLM046 at 4, 8 and 12 hours after osmotic treatment were higher than Hyola308. The highest rate of activity at 8 hours after stress was observed in SLM046 and the lowest activity was seen in Hyola308. At 24 hours after osmotic treatment, Hyola308 showed the highest activity at 60 mM concentration of substrate, and lowest rate was seen in SLM046. The results indicated that the high level of proxidase activity in SLN046 genotype in stress condition, led to the lower accumulation of H2O2 in comparison with Hoyla308.

کلیدواژه‌ها [English]

  • Antioxidant Enzymes
  • guaiacol
  • hydroponic
  • kinetic
  • polyethylene glycol

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سابقه مقاله

  • تاریخ دریافت: 15 اسفند 1394
  • تاریخ بازنگری: 09 اردیبهشت 1395
  • تاریخ پذیرش: 19 اردیبهشت 1395
  • تاریخ انتشار: 01 خرداد 1396

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