ارزیابی برنج‌های هوازی و ایرانی از لحاظ نشانگرهای پیوسته به صفات مرتبط با تحمل به خشکی و شوری و ارتباط آن‌ها با صفات جوانه‌زنی تحت تنش اسمزی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد اصلاح نباتات، گروه زراعت و اصلاح نباتات، دانشکدۀ کشاورزی، دانشگاه گیلان، رشت، ایران

2 استادیار اصلاح نباتات، گروه زراعت و اصلاح نباتات، دانشکدۀ کشاورزی، دانشگاه گیلان، رشت، ایران

چکیده

برنج­های هوازی به‌عنوان یک قابلیت کارآمد در کاهش دشواری‌های ناشی از بحران آب به شمار می‌آیند. بررسی و ارزیابی تفاوت‌های ژنتیکی این برنج­ها با رقم‌های برنج بومی ایرانی می­تواند در شناسایی نواحی ژنگانی (ژنومی) تأثیرگذار در تحمل به تنش خشکی سودمند باشد. در این پژوهش ضمن بررسی صفات جوانه­زنی ژنوتیپ­ها تحت تنش اسمزی، تنوع همردیف ‌ژنی (اللی) 26 نشانگر SSR پیوسته به QTLهای مرتبط با تحمل به خشکی یا شوری در 53 ژنوتیپ برنج شامل 31 ژنوتیپ برنج هوازی و 22 رقم برنج­ غرقابی (ایرانی) بررسی شد. در مجموع 118 همردیف ژنی چند­ شکل با میانگین 54/4 به ازای هر جایگاه ریز­ماهواره تولید شد. بالاترین میزان PIC محاسبه­شده با میزان 77/0 و 76/0و بیشترین تنوع ژنی با میزان 80/0 و 79/0 به ترتیب مربوط به نشانگرهای RM10793 و RM493 بودند. بررسی کلی آماره­های تنوع ژنتیکی نشان داد که دو نشانگر RM10793 و RM493 نسبت به دیگر نشانگرها مقادیر بالاتری داشتند و در جداسازی ژنوتیپ­ها نقش بارزتری ایفا کردند. گروه­بندی ژنوتیپ­ها نیز بر پایۀ ضریب جاکارد و با استفاده از الگوریتم اتصال همسایگی، آن‌ها را به دو گروه منتسب کرد. این گروه‌بندی با نتایج به‌دست‌آمده از تجزیۀ خوشه­ای بر پایۀ صفات جوانه­زنی با استفاده از الگوریتم وارد، همخوانی شایان‌توجهی داشت که این امر می­تواند بیانگر مؤثر بودن و تأیید نشانگرهای ریزماهواره مورد استفاده در جداسازی ژنوتیپ­ها از لحاظ تحمل به تنش اسمزی باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigation and comparison of aerobic and Iranian rice based on markers linked to traits related to drought and salinity tolerance and their relationship with germination traits under osmotic stress

نویسندگان [English]

  • Tayebe Raiesi 1
  • Atefeh Sabouri 2
1 Former M.Sc. Student of Plant Breeding, Department of Agronomy & Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
2 Assistant Professor of Plant Breeding, Department of Agronomy & Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
چکیده [English]

Aerobic rice is as useful potential in reducing of water problem. Comparison and investigation of genetic differences between Iranian rice varieties and aerobic rice can be useful in identification of genomic regions influencing in drought tolerance. In the present study in addition to an examination of germination genotypes under osmotic stress, allelic diversity were evaluated in 53  genotypes including 31 aerobic rice and 22 lowland Iranian rice varieties based on 26 SSR markers linked to QTL associated with drought and salinity tolerance. A total of 118 polymorphic alleles of microsatellite loci were produced with an average of 4.54 per marker. The highest calculated PIC level of 0.77 and 0.76 and the maximum range genetic diversity of 0.80 and 0.79 were related to RM10793 and RM493 respectively. Overview of genetic diversity statistics showed that two markers RM10793 and RM493 had higher values the other markers and more obvious role played in genotype differentiation. Grouping of genotypes according to Jaccard similarity coefficient using algorithms neighbor joining assigned them into two groups. This classification had significant compliance with the results of cluster analysis using the WARD algorithm based on germination traits. This may indicate the effectiveness and confirmation of used microsatellite markers in the separation of genotypes for osmotic tolerance.

کلیدواژه‌ها [English]

  • Cluster Analysis
  • microsatellite markers
  • Genetic diversity

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سابقه مقاله

  • تاریخ دریافت: 08 تیر 1394
  • تاریخ بازنگری: 10 بهمن 1394
  • تاریخ پذیرش: 12 بهمن 1394
  • تاریخ انتشار: 01 آذر 1395

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