ارزیابی تغییرپذیری‌های بیوشیمیایی و مولکولی شماری از ژنوتیپ‌های لوبیا (Phaseolus vulgaris L.) در شرایط تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 دانشیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

چکیده

به‌منظور بررسی­ تأثیر بیوشیمیایی و مولکولی تحمل به تنش خشکی ژنوتیپ­های K-S-31167، GE-288 و Naz به‌دست‌آمده از غربالگری آزمون جوانه­زنی در سطوح تنش خشکی 50 و 25 درصد ظرفیت زراعی به همراه شاهد در مرحلۀ گیاهچه­ای بررسی شد. تنش خشکی پنج هفته پس از کاشت بذر اعمال و ده روز پس از اعمال تنش نمونه­گیری از سه ­برگچۀ دوم انجام شد. نتایج نشان داد که تنش خشکی باعث تغییر در نشت یونی، پرولین و کربوهیدرات‌های محلول می­شود. ژنوتیپ متحمل K-S-31167 نسبت به دو ژنوتیپ به نسبت متحمل  GE-288و حساس Naz نشت یونی کمتر داشت که نشان‌دهندۀ پایداری غشایی بالاتر این ژنوتیپ در شرایط تنش است. محتوای پرولین بیشتر در ژنوتیپ K-S 31167 نشان‌دهندۀ سامانة پاداکسندگی (آنتی‌اکسیدانی) قوی‌تر این ژنوتیپ نسبت به دو ژنوتیپ GE-288 و Naz است. نتایج به‌دست‌آمده از سنجش کربوهیدرات­های محلول و بیان ژن­های اینورتاز واکوئلی و سوکروز سنتاز نیز نشان داد که افزایش و کاهش بیان این ژن­ها در پاسخ به تنش خشکی به ترتیب باعث افزایش و کاهش فعالیت آنزیم­های اینورتاز و سوکروز سنتاز و در نهایت افزایش و کاهش غلظت مواد محلول در یاخته­ها شده و به­عنوان یک نشانة سوخت‌وسازی (متابولیک) در پاسخ به تنش عمل می­کند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Biochemical and molecular changes in three genotypes of beans (Phaseolus vulgaris L.) under drought stress

نویسندگان [English]

  • Elham Soltani 1
  • Abdol Hadi Hosseinzadeh 2
  • Alireza Abbasi 2
1 Former M. Sc. Student, Department of Agronomy and Plant Breeding, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Associate Professor, Department of Agronomy and Plant Breeding, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

Drought stress is one of the major causes of reduction in crop production. Study physiological and biochemical processes related to drought stress can give better insight about mechanism of drought tolerance. To study the physiological and biochemical processes, three genotypes including K-S-31167, GE-288 and Naz were subjected to 3 level of drought stress based on 25, 50 and 100 percent of field capacity using CRD design with three replication using Factorial arrengment, Droght stress was applied five weeks after planting and lasted for 10 days. Second terifoliate were used for sampling. The results indicated significant changes in the electrolyte leakage (EL), prolin content (PC) and carbohydrates content (CC). Tolerant genotype (K-S-31167) had greater PC but lesser EL compare to relatively tolerant genotype (GE-288) and sensitive genotype (Naz). This indicates that tolerant genotype had higher membrane stability during stress. Results from measurement of soluble carbohydrate and expression of sucrose synthase and vacuolar invertase genes showed increase and decrease in the expression of these genes resulted in increase and decrease in the activity of the invertase and sucrose synthase enzyme which in turn influences the concentration of soluble materials which acts as metabolic signal in response to stress.

کلیدواژه‌ها [English]

  • common bean
  • Drought
  • expression gene
  • soluble carbohydrates
  • sucrose synthase
  • vacuolar invertase

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سابقه مقاله

  • تاریخ دریافت: 14 آذر 1394
  • تاریخ بازنگری: 15 دی 1394
  • تاریخ پذیرش: 03 بهمن 1394
  • تاریخ انتشار: 01 آذر 1395

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