بررسی فعالیت بعضی از آنزیم‌های پاداکسندگی و پراکسیداسیون چربی‌های غشا در ژنوتیپ‌های لوبیا چشم‌بلبلی (Vigna unguiculata L.) در شرایط عادی و تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری اصلاح نباتات، گروه اصلاح نباتات و بیوتکنولوژی، دانشکدۀ کشاورزی دانشگاه تبریز

2 استاد، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 دانشیار، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

چکیده

شناخت فرآیندهای فیزیولوژیکی مرتبط با تنش خشکی منجر به شناسایی راه­کارهای اساسی در مقاومت، حفظ ظرفیت عملکرد و رقم‌های متحمل به تنش خشکی خواهد شد. با این هدف تأثیر تنش خشکی بر ژنوتیپ­های لوبیا چشم­بلبلی در سال 1393 در دانشکدة کشاورزی دانشگاه تهران ارزیابی شد. پنج ژنوتیپ با قابلیت و پتانسیل عملکرد متفاوت در یک آزمایش کرت‌های خردشده (اسپلیت­پلات) در قالب طرح پایة بلوک کامل تصادفی در سه تکرار تحت سه سطح تنش خشکی (35، 65 و 100 درصد ظرفیت زراعی) قرار گرفتند. خشکی سبب کاهش RWC و افزایش فعالیت آنزیم­های پاداکسندگی (آنتی­اکسیدانی) و محتوای مالون­دی آلدهید شد. تنش خشکی سبب کاهش  RWC و افزایش MDA و فعالیت آنزیم­های پاداکسندگی شد که با افزایش سطوح تنش بر مقادیر آن‌ها افزوده شد اما برای آنزیم کاتالاز بیشترین میزان آنزیم در سطح تنش 65 درصد بود و با افزایش شدت تنش در سطح تنش 35 درصد ظرفیت زراعی میزان آن کاهش نشان داد. به صورتی‌که در سطح تنش 35 درصد ظرفیت زراعی بیشترین و کمترین  میزان محتوای آب نسبی به ترتیب مربوط به ژنوتیپ­های 210 و 313 بود، میزان MDA در این دو ژنوتیپ در این سطح تنش کمترین میزان را داشت برای آنزیم­های CAT، GPX و  APXژنوتیپ­های 210 و 291 بالاترین میزان را داشتند. بالا بودن فعالیت آنزیم­های پاداکسندگی در ژنوتیپ­های 210 و 291 همراه با پایین بودن میزان محتوای مالون­دی­آلدهید در این ژنوتیپ­ها، به‌احتمال زیاد نشان‌دهندة ظرفیت بالای این ژنوتیپ­ها برای حذف گونه­های فعال اکسیژن تولیدی و ثبات عملکرد و اجزای عملکرد در مقایسه با دیگر ژنوتیپ­ها است.

کلیدواژه‌ها

عنوان مقاله [English]

Assessment of antioxidant enzymes activity and peroxidation of membrane lipid in cowpea (Vigna unguiculata L.) genotypes under normal and drought stress condition

نویسندگان [English]

  • Khosro Mafakheri 1
  • Mohamad Reza Bihamta 2
  • Ali Reza Abbasi 3
1 Ph.D student Plant breeding in University of Tabriz
2 Professor, Department of Agronomy and Plant Breeding, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Associated Professor, Department of Agronomy and Plant Breeding, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

Understanding the physiological processes associated with drought stress lead to an understanding of the basic mechanisms of resistance, maintaining yield potential and contribute to the identification of drought tolerant cultivars. For this purpose, the effects of drought stress in cowpea genotypes was evaluated using five different genotypes with different yield potential in a pot experiment in split plot in a randomized complete block design with three replications under drought stress levels (35, 65 and 100% of field capacity) in the College of Agriculture, University of Tehran in 2014. Drought stress decreased RWC of cells and increase antioxidant enzyme and MDA content. Drought stress decreased RWC and increase MDA content and antioxidant enzyme activities by increasing levels of stress, but for catalase enzyme in stress levels the highest activity was 65%. With increasing stress and the stress level of 35% of field capacity, catalase activity exhibited higher reduction. Genotypes 210 and 313 had the lowest MDA level at 35% of field capacity and in these two genotypes enzymes CAT, GPX and APX 210 had the highest value. High levels of antioxidant enzyme activity in genotypes 210 and 291 associated with lower levels of MDA content in these genotypes, probably indicates the high potential of these genotypes to eliminate oxygen species production and yield stability compared with other genotypes.

کلیدواژه‌ها [English]

  • antioxidant enzyme
  • cowpea
  • Drought stress
  • Lipid Peroxidation

مراجع

  1. Abdul Jaleel, C., Riadh, K., Gopi, R., Manivannan, P., Ines, J., Al-Juburi, H.J., Chang-Xing, X., Hong-Bo, S. & Panneerselvam, R. (2009). Antioxidant defense responses: physiological plasticity in higher plants under abiotic constraints. Acta Physiologiae Plantarum, 31, 427-436.
  2. Abedi, T. & Pakniat, H. (2010). Antioxidant Enzyme Changes in Response to Drought Stress in Ten Cultivars of Oilseed Rape (Brassica napus L.). Czech Journal of Genet Plant Breed, 46(1), 27-34.
  3. Aebi, H. (1984). Catalase in vitro. Methods in Enzymology. ELSEVIER. 105, 121-126.
  4. Ariano, S., Bartolomeo, D., Cristos, X. & Andras, M. (2005). Antioxidant defenses in Olive trees during drought stress: changes in activity of some antioxidant enzymes. Functional Plant Biology, 32, 45-53.
  5. Asada, K. (1992). Ascorbate peroxidase-a hydrogen peroxide acavenging in plant. Physiologia platarum, 85, 235-241.
  6. Bailly, C. (2004). Active oxygen species and antioxidants in seed biology. Seed Science Research, 14, 93-107.
  7. Borsani, O., Valpuesta, V. & Butella, M.A. (2001) Evidence for the role of salisylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings. Plant Physiology, 126, 1024-1030.
  8. Bradford, M.M. (1979). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.
  9. Carmak, I. & Horst, J.H. (1991). Effects of aluminum on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiol Plant, 83, 463-8.
  10. Cameron, D. (1999). The effect of different irrigations on water relation and growth in Rododenderon. New Phytologist, 137, 90-95.
  11. Campos, P.S., Quartin, V., Ramalho, J.C. & Nunes, M.A. (2003). Electrolyte leakage and lipid degradation account for cold sensitivity in leaves of Coffea sp. Journal of Plant Physiology. 160, 283-292.
  12. Canfield, T.J., Brunson, E.L., Dwyer, F.J., Ingersoll, C.G. & Kemble, N.E. (1998). Assessing sediments from the upper Mississippi River naviga­ tional pools using a benthic community evaluation and the sediment quality triad approach. Arch Environ Contam Toxic, 0135, 202-212
  13. Chance, A. & Maehly, A.C. (1955). Assay of catalases and proxidase. Methods Biochem Anal, 2, 764-775.
  14. Dat, J.F., Lopez–Delgado, H., Foyer, C.H. & Scott, I.M. (1998). Parallel changes in H2O2and catalase during thermo tolerance induced by salicylic acid or heat acclimation in mustard seedlings. Plant Physiology, 116, 1351-1357.
  15. Dedio, W. (1975). Water reiations in wheat leaves as screening tests for drought resistance, Canadian Journal of Plant Scienc, 55, 369-378.
  16. Demiral, T. & Turkan, I. (2005). Comparative lipid per oxidation, antioxidant defense systems and praline content in roots of two rice cultivars differing in salt tolerance. Environmental and Experimental Botany, Elsevier, 53, 247-257.
  17. Dhindsa, R.S. (1991). Drought stress, Enzymes of Glutathione Metabolism, Oxidation Injury, and Protein Synthesis in Turtula ruralis. Plant Physiology, 95, 648-651.
  18. Faize, M., Burgos, L., Faize, L., Piquerasm, A., Nicolas, E., Barba-Espin, G., Clemente-Moreno, M. J., Alcobendas, R., Artlip, T. & Hernandez, J.A. (2011) Involvement of cytosolic ascorbate peroxidase and Cu/Zn-superoxide dismutase for improved tolerance against drought stress. Journal of Experimental Botany, 62(8), 2599-2613.
  19. Graça, J.P., Rodrigues, F.A., Farias, J.R.B., Oliveira, M.C.N., Hoffmann-Campo, C.B. & Zingaretti, S.M. (2010). Physiological parameters in sugarcane cultivars submitted to water stress. Brazilian Journal of Plant Physiology, 22(3), 189-197, ISSN 1677-0420.
  20. Genadii, B., Irina, V., Anna, I.  & Victor, K.  (2002). Accumulation of dehydrin-like protein in the mitochondria of cereals inresponse to cold, freezing, drought and ABA treatment. Plant Biology, 2, 157-170.
  21. Gill, S.S. & Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48, 909-930.
  22. Guo, Z., Ou, W., Lu, S. & Zhong, Q. (2006). Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiology and Biochemistry, 44, 828-836.
  23. Hsu, S.Y. & Kao, C.H. (2003).  Differential  effect  of  sorbitol  and  polyethylene  glycol  and  antioxidant  enzymes  in  rice leaves. Plant Growth Regulation, Springer, 39, 83-90.
  24. Hsiao, T.C. (1973). Plant responses to water stress. Ann. Rev. Plant Physiology, 24, 519-570.
  25. Ilampour, S. (1992). Irrigation scheduling and estimating evaporation sweating cowpea of farm (Vigna sinensis L.) by using the temperature of the green plant cover. MS Thesis, Department of Agriculture. ShirazUniversity. Iran. p166.
  26. Israr, M. & Sahi, S.V. (2006). Ant oxidative responses to mercury in the cell cultures of Sesbania drummondii. Plant Physiology and Biochemistry, 44, 590-595.
  27. Jiang, Y. & Huang, B. (2001). Drought and Heat Stress Injury to Two Cool-Season Turf grasses in Relation to Antioxidant Metabolism and Lipid Peroxidation. Crop Science, 41, 436-442.
  28. Kazemi Shahandashti, S.S., Mali Amiri, R. & Zinali Khanghah, H. (2011). Assessment some indicators of cell damage under cold stress in pea Jam. Genetic Novin Journal, 6(4), 70-77. (in Farsi)
  29. Khani, M.R., Heidari Sharif Abad, H., Madani, H. & Amini, Z. (2011). The comparison between different levels of drought stress on yield and components yield of 5 cultivars of sesame in tropical region. The first national conference on new issues in agriculture, Islamic Azad University Saveh branch, Saveh, Iran.http://www.civilica.com/Paper-NCNCA01-NCNCA01_217.html.
  30. Kubo, A., Aono, M., Nakajima, N., Saji, H., Tanaka, K. & Kondo, N. (1999). Differential responses in activity of antioxidant enzymes to different environmental stresses in Arabidopsis thaliana. Journal of Plant Research, 112, 279-290.
  31. Lee, D. H., Kim, Y. S. & Lee, C. B. (2001). The inductive responses of the antioxidant enzymes by salt stress in the rice (Oryza sativa L.), Journal of Plant Physiology, 158, 737-745.
  32. Luna, C.M., Pastori, G.M., Driscoll, S., Groten, K., Bernard, S. & Foyer, C.H. (2004). Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat. Journal of Experimental Botany, 56, 417-423.
  33. Majnoon Hoseini, N. (2008). Culture and Production Leguminous, Tehran University Publication Jahad. (in Farsi)
  34. Mafakheri, Kh. Bihamta, M. R., Abbasi, A. R. & Rasolnia, A.R. (2015). Genetic diversity of cowpea (Vigna unguiculata (L.) Walp) Germplasm Based on Agronomic and Morphological Traits. Seed and Plant, Improvement Journal, 1(31), 135-162. (in Farsi)
  35. McDonald, M. B. (1999). Seed deterioration: physiology, repair, and assessment. Seed Science and Technology, 27(11), 177-237.
  36. Mittler, R. (2002). Oxidative stress, antioxidant and stress tolerance. Ann. Rev. Plant Science., 7, 405-415.
  37. Moran, J.F., Becana, M.I., Iturbe-Ormaetxe, S., Frechilla, R., Klucas, V. & Aparicio-Tejo, P. (1994). Drought induces oxidative stress in pea plants. Plant Science, 194, 346-352.
  38. Moore, K. & Roberts, L.J. (1998). Measurement of lipid peroxidation. Free Radical Research, 28, 659-71.
  39. Patel, P.K. & Hemantaranjan, A. (2012). Antioxidant Defence System in Chickpea (Cicer arietinum L.) Influence by Drought Stress Implement at Pre- and Post-anthesis Stage. American Journal of Plant Physilogy, 7(4), 164-173.
  40. Polle, A. (2001). Dissecting the superoxide dismutase-ascorbate-glutathione pathway in chloroplasts by metabolic modeling: Computer simulation as a step towards flux analysis. Plant Physiology, 126, 445-462.
  41. Ranieri, A., Castagna, A., Pacini, J., Baldan, B., Mensuali Sodi, A. & Soldatini, G.F. (2003). Early production and scavenging of hydrogen peroxide in the apoplast of sunflower plants exposed to ozone. Jornal of Experimental Botany, 54, 2529-2540.
  42. Rajendra, P., Kandpal, C.S., Vaidyanathan, M., Udayakumar, K.S. & Appaji, R. (1991). Alterations in the activities of the  enzymes  of  proline  metabolism  in  Ragi(Eleusine coracana)  leaves  during  water stress.  Journal of Biosciences, 3, 361-370.
  43. Rostami, A.A. & Rahemi, M. (2013). Screening Drought Tolerance in Caprifig Varieties in Accordance to Responses of Antioxidant Enzymes. World Applied Sciences Journal, 21(8), 1213-1219.
  44. Sairam, R.K., Deshmukh, P.S. & Saxena, D.C. (1998). Role of antioxidant systems in wheat genotypes tolerance to water stress. Plan Biology, 41(3), 387-394.
  45. Sairam, R.K. & Srivastava, G.C. (2002). Changes in antioxidant activity in sub-cellular fractions of tolerant and susceptible wheat genotypes in response to long term salt stress. Plant Science, Elsevier, 162, 897-904.
  46. Saglam, A., Saruhan, N., Terzi, R. & Kadioglu, A. (2011). The Relations between Antioxidant Enzymes and Chlorophyll Fluorescence Parameters in Common Bean Cultivars Differing in Sensitivity to Drought Stress. Russian Journal of Plant Physiology, 58(1), 60-68.
  47. Sayfzadeh, S., Habibi, D., Taleghani, D.F., Kashani, A., Vazan, S., Sadre Qaeni, S.H., Khodaei, A.H., Boojar, M.M.A. & Rashidi, M. (2011). Response of Antioxidant Enzyme Activities and Root Yield in Sugar Beet to Drought Stress. International Journal of Agriculture & Biology, 1560-8530, ISSN Online, 1814-9596.
  48. Silva, M.A., Jifon, J.L., Silva, J.A.G. & Sharma, V. (2007). Use of physiological parameters as fast tools to screen for drought tolerance in sugarcane. Brazilian Journal of Plant Physiology, Vol.19, No.3, (July/September 2007), pp. 193-201, ISSN 1677-0420.
  49. Svetleva, D., Krastev, V., Dimova, D., Mitrovska, Z., Miteva, D., Parvanova, P. & Chankova, S. (2012). Drought tolerance of Bulgarian common bean genotypes, charactrized by some biochemical markers for oxidative stress. Journal of Central European Agriculture, 13(2), 349-361.
  50. Smirnoff, N. (1993). The role of active oxygen in the response of plants to water deficit and desiccation. New Phytologist, 125(1), 27-58.
  51. Sharma, P. & Dubey, R.S. (2005). Drought induces oxidative stress and enhances theactivities of antioxidant enzymes in growing rice seedlings. Plant Growth Regulation, Springer, 46, 209-221.
  52. Shahram, A. & Daneshi, N. (2005). The most appropriate amount of water needed in irrigation and agriculture agricultural white beans. Ninth Congress of Soil Science. Tehran, Iran. http://www.civilica.com/Paper-SSCI09-SSCI09_457.html. 
  53. Szilagyi, L. (2003). Influence of drought on seed yield components in common bean, Blug. J. Plant Phsiology. Special Issue, 320-330.
  54. Tayefi-Nasrabadi, H., Dehghan, G., Daeihassani, B., Movafegi, A. & Samadi, A. (2011). Some biochemical properties of guaiacol peroxidases as modified by salt stress in leaves of salt-tolerant and salt-sensitive safflower (Carthamus tinctorius L.cv.) cultivars. African Journal of Biotechnology, 10(5), 751-763.
  55. Terzi, R., Saglam, A., Kutlu, N., Nar, H. & Kadioglu, A. (2010). Impact of soil drought stress on photochemical efficiency of photosystem II and antioxidant enzyme activities of Phaseolus vulgaris cultivars. Turkish Journal of Botany, 34, 1-10.
  56. Turkan, I., Bor, M., Ozdemir, F. & Koca, H. (2005). Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Science, 168, 223-231.
  57. Vanai, S., Siosemardeh, A. & Haidari, GH.R. (2011). The effects of cold stress during seed germination and seedling antioxidant enzyme activities and some physiological traits in pea (Cicer arietinum). Iranian Journal of Field Crops Research, 9(3), 514-524.
  58. Yasar, F., Uzal, O. & Ozpay, T. (2010). Changes of the lipid peroxidation and chlorophyll amount of green bean genotypes under drought stress. African Journal of Agricultural Research, 5(19), 2705-2709.
  59. Zhang, J. & Kirkham, M.B. (1996). Antioxidant responses to drought in sunflower and sorghum seedlings. New Phytologist, 132, 361-373.
  60. Zlatev, Z.S., Lidon, F.C., Ramalho, J.C. & Yordanov, I.T. (2006). Comparison of resistance to drought of three bean cultivars. Biology Plantrum, 50(3), 389-394.
علوم گیاهان زراعی ایران
دوره 47، شماره 2 - شماره پیاپی 2
تابستان 1395
صفحه 217-232

فایل ها

سابقه مقاله

  • تاریخ دریافت: 05 خرداد 1394
  • تاریخ بازنگری: 29 آبان 1394
  • تاریخ پذیرش: 01 آذر 1394

اشتراک گذاری

ارجاع به این مقاله

آمار