شناسایی و ارزیابی الگوی بیان ژن‌های دخیل در مسیر‌های سیگنال‌دهی در آجیلوپس (Aegilops tauschii) تحت تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 استاد پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 استادیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

4 دانشیار گروه زراعت و اصلاح نباتات دانشگاه شهید باهنر کرمان

5 گروه سلولهای بنیادی، مرکز تحقیقات علوم سلولی جهاد دانشگاهی، پژوهشگاه رویان، تهران

6 دانشیار، بخش ژنومیکس پژوهشکده بیوتکنولوژی کشاورزی کرج

چکیده

شور شدن زمین­های کشاورزی یکی از مهم‌ترین عامل‌های محدودکنندۀ تولیدات کشاورزی و تهدیدکنندۀ امنیت غذایی در کشورهای جهان به­شمار می‌آید. فعال شدن مسیرهای سیگنالی نخستین رخداد در شناسایی تنش­های محیطی برای گیاهان به­شمار می‌آید. شناسایی اجزای شبکۀ سیگنالی در پاسخ به تنش شوری با استفاده از روش RNA Seq در گیاه آجیلوپس به‌عنوان منشأ ژنگان (ژنوم) DD در گندم، اهمیت زیادی دارد. توالی­یابی RNA به همراه استفاده از نرم‌افزار MapMan برای تفسیر عملکردی نتایج بیان ژن روش مناسب برای بررسی‌های ژن‌نامه شناسی (ژنومیکس) کارکردی در گیاهان به شمار می‌آید. لذا در این تحقیق با استفاده از کتابخانۀ تهیه‌شده از RNA استخراج‌شده از گیاهان کنترل و تحت تنش، اقدام به شناسایی و ارزیابی کمی ژن‌های دخیل در فرآیندهای سیگنالی شد. نتایج بیان ژن نشان داد که از بین 4506 رونوشت دارای تغییر بیان معنی­دار، فرآیندهای سیگنالی با 603 عدد BIN (59/11 درصد) بیشترین شمار را به خود اختصاص داد. همچنین مشخص شد که از بین اجزای شبکۀ سیگنالی، گیرندة (رسپتور) کینازها بیشترین شمار (222 عدد BIN) از ژن‌های تغییربیان‌یافته را شامل می­شوند که درواقع بیان­کنندۀ اهمیت آن‌ها است. این تحقیق به‌عنوان یک بررسی بنیادی به شمار می‌آید که زمینه برای انجام بررسی‌های انتقال ژن‌های مقاومت به شوری به گیاه گندم را فراهم می‌کند.

کلیدواژه‌ها


عنوان مقاله [English]

Expression profiling of genes involved in signaling process in Aegilops tauschii under salinity stress

نویسندگان [English]

  • mehdi mansouri 1
  • Mohamad Reza Naghavi 2
  • Houshang Alizadeh 3
  • Ghasem Mohammadinejad 4
  • Seyed Ahmad Mousavi 5
  • Ghasem Hosseini Salekdeh 6
1 University of Tehran
چکیده [English]

Salinization of crop lands is one of the most important reasons for reduction in productivity and threats food security. Activation of signaling process in plants is the first step during sense environmental cues and stress signals. Identification of the components of plant intracellular signaling mechanisms, pathways, and networks in response to salt stress in Aegilops tauschii (the D genome donor of bread wheat) by transcriptome analysis tools such as RNA Seq method is very worthwhile. RNA Seq method and functional annotation of data by MapMan suite is an efficient strategy to functional genomics analyses. In order to identify and evaluate some genes involved in signaling process, we performed library construction for two cDNA libraries from leaves of seedlings treated with 200 mM NaCl and control. Results of differential expression showed that out of 4506 differentially expressed genes, 603 (11/59%) BINs assigned to signaling process. It also became clear that receptor kinases are the largest number (222 transcripts) of differentially expressed genes in signaling process components. This study is a fundamental research that provide worthwhile functional sequences for gene transferring study to improve salinity tolerant in bread wheat.

کلیدواژه‌ها [English]

  • salinity
  • signaling process
  • sequencing
  • RNA Seq
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