بررسی تغییرپذیری پلی‌آمین‌ها تحت تنش خشکی در لوبیای قرمز (Phaseolus vulgaris L.)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری بیوتکنولوژی کشاورزی، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 دانشیار گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

چکیده

در این تحقیق تغییرپذیری پلی‌آمین‌های پوترسین (put)، اسپرمیدین (spd)، اسپرمین (spm) و نشت الکترولیت (EL) در سه ژنوتیپ K-S-31167، GE-288 و NAZ لوبیا تحت تنش خشکی بررسی شد. تنش خشکی در مرحلۀ گلدهی بر پایۀ ظرفیت زراعی و در گلخانه انجام شد. در هر سه ژنوتیپ با افزایش شدت تنش، محتوای پوترسین و اسپرمین افزایش یافت. بیشترین افزایش پوترسین در ژنوتیپ حساس به خشکی NAZ و بیشترین افزایش اسپرمین در ژنوتیپ مقاوم به خشکی K-S-31167 مشاهده شد. دو ژنوتیپ K-S-31167 و NAZ افزایش پیوسته در محتوای اسپرمیدین را نشان دادند اما در ژنوتیپ GE-288 کاهش و پس‌ازآن افزایش در محتوای اسپرمیدین مشاهده شد. ژنوتیپ حساس (NAZ) گرایش به تجمع پوترسین و ژنوتیپ مقاوم (K-S-31167) گرایش به تجمع اسپرمین نسبت به دیگر پلی‌آمین‌ها دارند. تنش باعث افزایش در نشت الکترولیت در هر سه ژنوتیپ شد. نتایج نشان داد که ژنوتیپ مقاوم به خشکی K-S-31167 نشت الکترولیت کمتری نسبت به ژنوتیپ حساس NAZ داشت.

کلیدواژه‌ها


عنوان مقاله [English]

Polyamine changes analysis under drought stress in common bean (Phaseolus vulgaris L.)

نویسندگان [English]

  • Mohsen Mombeni 1
  • Alireza Abbasi 2
چکیده [English]

In this research, we studied polyamines including putrescine (put), spermidine (spd) and spermine (spm) changes in three common bean (Phaseolus vulgaris) genotypes, K-S-31167, GE-288 and NAZ under drought stress. Our result showed steadily increase in putrescine (put) and spermine (spm) in all three genotypes.  GE-288 genotype showed decrease and then increasing in spermidine (spd) content but K-S-31167 and NAZ genotypes showed steadily increasing in spermidine (spd). NAZ genotype accumulated putrescine more than other polyamines and showed the highest amount of putrescine in compare with other genotypes. K-S-31167 genotype accumulated the highest spermine content in compared with other genotypes. Our result showed significant increase in electrolyte leakage (EL) in all three genotypes. K-S-31167 as drought tolerant genotype and ZAZ as drought sensitive genotypes showed the lowest and the highest increase in EL, respectively.

کلیدواژه‌ها [English]

  • common bean
  • Drought stress
  • electrolyte leakage
  • polyamine
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