بررسی تنوع ژنتیکی ژنوتیپ‌های مختلف Brassica spp. با استفاده از نشانگر‌های NBS-LRR

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس ارشد، دانشگاه علوم کشاورزی و منابع طبیعی ساری

2 استادیار، دانشگاه علوم کشاورزی و منابع طبیعی ساری

3 دانشجوی دکتری پژوهشکدۀ ژنتیک و زیست‌فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی

چکیده

بیماری‌هایزیادیدردنیا،بستهبهشرایطاقلیمی، گیاهکلزاراتهدیدمی‌کند وموجبواردآمدنخسارتاقتصادیبهآنمی‌شود. چنانچه توارث ژنتیکی کافی موجود باشد،مؤثرترین روش برای کنترل بیماری، ایجاد ژنوتیپ مقاوم است بنابراین، بررسی تنوع­ ژنتیکی ژرم‌پلاسم موجود، یکی از روش‌های مناسب مدیریت منابع ژنتیکی است. روش نمایه‌برداری NBSموتیف‌های محافظت‌شده در دمین‌های عملکردی خانوادۀ ژنی را مشخص می‌کند، بنابراین بررسی تنوع ژنتیکی، در داخل یا اطراف ژن‌های عملکردی انجام می‌گیرد. در این مطالعه، تنوع ژنتیکی 46 ژنوتیپ از جنس Brassica با استفاده از هشت ترکیب آغازگری نشانگر NBS  ارزیابی شد. در مجموع 790 مکان نشانگری مشاهده شد که از این تعداد، 708 مکان چندشکل بود. ضرایب تشابه در دامنۀ 79/0 تا 54/0 با متوسط 665/0 متغیر بود. دندروگرام‌های حاصل از تجزیۀ ­خوشه‌ای با روش UPGMA و همچنین تجزیه به مؤلفه‌های اصلی، ژنوتیپ‌ها را از یکدیگر تفکیک کرد. نتایج تجزیۀ خوشه‌ای نشان داد که ژنوتیپ‌ها در شش گروه قرار می‌گیرند. در مجموع، این نتایج نشان می‌دهد که نمایه‌برداری NBSیک ابزار معتبر طبقه‌بندی برای بررسی تنوع ژنتیکی است.

کلیدواژه‌ها


عنوان مقاله [English]

Assessment of genetic diversity in different genotypes of Brassica spp. using NBS-LRR markers

نویسندگان [English]

  • Mahsa Mohammad Jani Asrami 1
  • Hamid Najafi Zarrini 2
  • Seyyd Hamidreza Hashemi 3
1 Graduate in Plant Breeding, University of Agriculture Sciences and Natural Resources Branch of Sari
2 Assistant Professor in Plant Breeding, University of Agriculture Sciences and Natural Resources Branch of Sari
3 Ph.D. Student, Genetics & Agricultural Biotechnology Institute of Tabarestan, University of Agriculture Sciences and Natural Resources Branch of Sari
چکیده [English]

  Breeding for resistance is one of the main approaches to control diseases. In this approach, there is a necessary for existence a sufficient genetic variability of resistance genes. Accordingly, assessing the genetic variability in the available germplasm is needed to manage genetic resources. Molecular markers are effective tools to investigate genetic diversity for resistance to pathogens. NBS-LRR (Nucleotide-Binding Site- Leucine Rich Repeat) marker can be used to study genetic variability. Those markers specifically targets chromosome regions containing R-genes and R-gene analogues. In this study eight primer combinations of NBS markers were used to assess the genetic diversity among 46 accessions of Brassica spp. As result, NBS markers amplified 790 DNA bands that more than 89% bands were polymorphism. Average PIC (Polymorphism Information Content) and average MI (Marker Index) were 0.29 and 26.36 respectively. Cluster analysis based on Simple Match Coefficient and UPGMA algorithm showed that there is wide variation in the evaluated samples. The average of similarity based onNBS markers was 0.66. These high levels of diversity of these amplified NBS markers indicate a high potential of these markers for discrimination the Brassica genotypes.

کلیدواژه‌ها [English]

  • Brassica spp
  • Genetic diversity
  • NBS Profiling
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