کارایی برخی جدایه‌های ریزوبیومی بومی مولد ACC-دآمیناز در تعدیل تأثیرات سوء اتیلن تنشی بر شاخص‌های رشد کلزا (.Brassica napus L)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس ارشد گروه مهندسی علوم خاک، پردیس کشاورزی و منابع طبیعی دانشگاه تهران

2 استاد گروه علوم و مهندسی خاک، پردیس کشاورزی و منابع طبیعی دانشگاه تهران

3 استادیار گروه علوم و مهندسی خاک پردیس کشاورزی و منابع طبیعی دانشگاه تهران

چکیده

     در این پژوهش، توانایی 32 جدایۀ ریزوبیومی بومی (26 جدایه متعلق به گونۀ سینوریزوبیو[j1] م ملیلوتی (Sm)، 4 جدایه متعلق به گونۀ ریزوبیوم لگومینوزاروم بیووار فازئولی (Rlp) و 2 جدایه متعلق به گونۀ ریزوبیوم لگومینوزاروم بیووار ویسیه (Rlv)) متحمل به شوری، برای استفاده از ACC به‌عنوان تنها منبع تأمین‌کنندۀ نیتروژن در محیط کشت RMM ارزیابی شد. براساس نتایج، 25 جدایه برای ACC-دآمیناز مثبت ارزیابی شدند و جدایه­ها براساس رشد در محیط RMM حاوی ACC در سه گروه قوی، متوسط و ضعیف طبقه‌بندی شدند. در مرحلۀ بعد، آزمون گلخانه‌ای در گروه علوم و مهندسی خاک دانشگاه تهران در سال 1390، با هدف بررسی کارایی 2 جدایۀ قوی (Rlp281R و Sm103R)، 1 جدایۀ ضعیف (Sm29R) و به‌همراه تیمار شاهد، برای بهبود شاخص‌های رشد کلزا تحت تنش شوری 0، 3، 6 و 9 دسی‌زیمنس بر متر، به‌صورت فاکتوریل در قالب طرح بلوک‌های کامل تصادفی در سه تکرار اجرا شد. نتایج نشان داد مایه‌زنی با جدایۀ Rlp281R حتی در شوری 9 دسی‌زیمنس بر متر، به‌طور معنی‌داری وزن تر و خشک ریشه (به‌ترتیب 32 و 6/62 درصد)، وزن تر و خشک اندام هوایی (22 و 5/23 درصد)، ارتفاع بوته (49 درصد) و جذب نیتروژن (50 درصد) را نسبت به شاهد افزایش داد. همچنین در این سطح شوری، مایه‌زنی با Sm103R جذب فسفر را 5/34 درصد نسبت به شاهد افزایش داد. همبستگی مثبتی بین فعالیت ACC-دآمیناز جدایه­ها و شاخص‌های رشد گیاه به‌ویژه زیست‌تودۀ ریشه مشاهده شد (ضریب همبستگی پیرسون >82/0). این نتایج نشان می‌دهد مایه‌زنی با جدایه­های دارای فعالیت بالای ACC-دآمیناز در افزایش شاخص‌های رشد کلزا موفق‌تر عمل می‌کند و تأکیدی بر این است که استفاده از این جدایه‌ها می‌تواند به‌عنوان راهکار بیولوژیک در کاهش تأثیرات تنش شوری مطرح باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Effectiveness of some native Rhizobium spp. containing ACC-deaminase in amelioration of negative effects of stress ethylene on growth parameters in Oilseed rape (Brassica napus L.)

نویسندگان [English]

  • Davood Saghafi 1
  • Hossein Ali Alikhani 2
  • Babak Motesharezadeh 3
1 M.Sc. Graduate Student, at Soil Science Department, University College of Agriculture and Natural Resources, University of Tehran
2 Professor at Soil Science Department, University College of Agriculture and Natural Resources, University of Tehran
3 Assistant Professor at Soil Science Department, University College of Agriculture and Natural Resources, University of Tehran
چکیده [English]

In this study, the ability of 32 rhizobium strains (26 strain of Sinorhizobium meliloti (SM)), 4 strains of Rhizobium leguminosarum biovar phaseoli (Rlp) and 2 salt tolerance strains of Rhizobium leguminosarum  biovar viciae (Rlv) for use of ACC as individual nitrogen source in RMM media was evaluated. Based on the results, 25 strains were found positive for ACC-deaminse, and strains according to growth in the RMM media containing ACC were categorized into three groups of strong, medium, and weak. In the next step, in 2011 a series of greenhouse experiments were conducted for investigating the effectiveness of two strains from strong group (R281Rlp and R103Sm) and one strain from weak group (R29Sm) with control treatment, for the promotion of growth parameters of canola under the salinity levels of 0, 3, 6, and 9 ds/m in factorial format experiment as randomized complete block design with three replications. The Results showed that R281Rlp inoculation, even at higher salinity (9dS/m), significantly increased wet and dry weight of root (32 and 62.6%, respectively), wet and dry weight of shoot ( 22 and 23.5%, respectively), height (49%) and nitrogen uptake (50%) compared to control treatment. Furthermore, at this level of salinity, R103Sm inoculation increased the uptake of Phosphorous up to 34.5% compared to control. A positive correlation was observed between ACC-deaminase activity of bacterial isolates and plant growth parameters; especially root biomass, (Pearson correlation coefficients > 0.82). These results revealed that the strains containing high ACC-deaminase activity were more successful in improving the growth parameters of canola seedling, which confirms that using of these strains may be suggested as a biologic alternative to the amelioration of increasingly saline stress.

کلیدواژه‌ها [English]

  • ACC-deaminase
  • Growth parameters
  • Rhizobia
  • salt stress
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