مکان‌یابی QTLهای کنترل‌کنندۀ یون‌های سدیم و پتاسیم در ریشه و اندام هوایی گندم تحت شرایط نرمال و تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری اصلاح نباتات، دانشکدۀ کشاورزی دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران

2 استاد، پژوهشکدۀ بیوتکنولوژی کشاورزی کرج

3 دانشیار پژوهشکدۀ بیوتکنولوژی کشاورزی کرج

4 استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

5 استادیار، پژوهشکدۀ بیوتکنولوژی کشاورزی کرج

6 مربی مؤسسۀ تحقیقات اصلاح و تهیۀ نهال و بذر کرج

چکیده

مشخص شده است که خروج یون سدیم و نسبت K+/Na+ بیشتر در گندم با تحمل به شوری ارتباط دارد، بنابراین به‌منظور شناسایی QTLهای دارای تأثیرات افزایشی برای مقدار یون‌های سدیم و پتاسیم در ریشه و اندام هوایی گندم، 319 لاین نوترکیب خالص (RIL F7) گندم نان، حاصل از تلاقی رقم روشن (متحمل به شوری) و رقم فلات (حساس به شوری)، به همراه والدین و دو شاهد (ارگ، مغان 3) در قالب دو طرح مجزا (تنش و نرمال) به‌صورت بلوک کامل تصادفی با سه تکرار در شرایط گلخانه‌ای در سال 1391 ارزیابی شدند. صفات مورد بررسی در این تحقیق عبارت بودند از مقدار سدیم و پتاسیم ریشه و اندام هوایی، نسبت پتاسیم به سدیم ریشه و اندام هوایی و نسبت جابه‌جایی سدیم و پتاسیم از ریشه به اندام هوایی. از 730 نشانگر (709 نشانگر دارت و 21 نشانگر SSR) در تهیۀ نقشۀ پیوستگی استفاده شد. طول نقشۀ پیوستگی 71/4505 سانتی‌مورگان و متوسط فاصلۀ بین دو نشانگر 17/6 سانتی‌مورگان بود. در مجموع 31  QTLافزایشی به‌وسیلۀ نرم‌افزار QTL Cartographer با استفاده از ارزش فنوتیپی هر یک از تیمارها به‌طور جداگانه شناسایی شد. نتایج نشان داد که مسیرهای بیوشیمیایی تجمع یون سدیم و پتاسیم به احتمال زیاد جدا هستند و نیز صفات مقدار سدیم اندام هوایی، نسبت جابه‌جایی سدیم و پتاسیم از ریشه به اندام هوایی را می‌توان به‌عنوان شاخص‌های مهمی در انتخاب ارقام متحمل به حساس [m1] در شرایط تنش شوری به‌کار برد.



 [m1]؟؟؟

کلیدواژه‌ها

عنوان مقاله [English]

QTL mapping of genes controlling Na+ and K+ concentration in roots and shoots of wheat under normal and salt stress conditions

نویسندگان [English]

  • Bahram Masoudi 1
  • Eslam Majidi Hervan 2
  • Mohsen Mardi 3
  • Mohammad-Reza Bihamta 4
  • Mohammad-Reza Naghavi 4
  • Babak Nakhoda 5
  • Ashkboos Amini 6
1 Ph.D. Student, Department of plant breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Prof., Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
3 Associate Prof., Department of Genomics, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
4 Professors, Department of plant breeding, The University of Tehran, Karaj, Iran.
5 Prof., and Assistant Prof., Department of Molecular Physiology, Agricultural
6 Instructor, Seed and Plant Research Institute, Karaj, Iran
چکیده [English]

In wheat Na+ exclusion and K+/Na+ have shown to be associated with salinity tolerance, therefore in order to identify QTLs with additive effect for Na+ and K+ concentration traits in roots and shoots of wheat, 319  bread wheat recombinant inbred lines (RIL F7), derived from a cross between Roshan cultivar (salt tolerant) and Falat cultivar (salt sensitive), along with their parents and 2 checks (Arg and Moghan3) were studied in 2 separate randomized complete block designs (normal and stress) with 3 replications in greenhouse in 2012. Traits measured included shoot and root Na+ and K+ concentration, K+/Na+ ratio in root and shoot and root to shoot Na+ and K+ concentrations. Linkage map was constructed with 730 markers (709 DArt markers and 21 SSR markers). The linkage map spanned 4505.71 cM with an average distance of 6.17 cM between adjacent markers. A total of 31 additive QTLs were identified by QTL Cartographer program using single-environment phenotypic values. Results indicated that the biochemical pathways for Na+ and K+ accumulation are highly likely to be independent. Also, results indicated that shoot Na+ concentration and root to shoot Na+ and K+ concentrations could be used as selection criteria between tolerant and sensitive cultivars in salt stress condition.
 

کلیدواژه‌ها [English]

  • Physiological traits
  • QTL
  • salt stress
  • bread wheat

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سابقه مقاله

  • تاریخ دریافت: 27 فروردین 1392
  • تاریخ بازنگری: 29 اردیبهشت 1394
  • تاریخ پذیرش: 20 مهر 1392

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