عنوان مقاله [English]
نویسندگان [English]چکیده [English]
Responses of plants to salinity stress and development of salt tolerance are extremely complex and various mechanisms appear to be involved. We employed a proteomic approach to study the mechanism of plant responses to salinity in a sensitive cultivar of barley (527 line). Three-week-old seedlings were treated with 300 mM NaCl for 3 weeks. Total proteins of fourth leaf were extracted and separated by two-dimensional gel electrophoresis. 377 protein spots were reproducibly detected, including 55 that were up-regulated and 69 down-regulated. Using MALDI-TOF-TOF MS, 25 proteins involved in many cellular functions were identified. These proteins were; oxygen-evolving enhancer protein, methionine sulfoxide reductase, chloroplast RNA-binding proteins, chloroplast-localized cyclophilin, rubisco, ribulose bisphosphate carboxylase/oxygenase activase, Nascent polypeptide associated complex alpha, thioredoxin, dehydroascorbate reductase, oxalate oxidase-like protein or germin-like protein, profilin, universal stress proteins and ribosomal protein.