شناسایی ژن‌های مقاومت چهار نوع از بیماری‌های مهم در لاین‌های گوجه‌فرنگی (Solanum lycopersicum L.)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران، کرج.

2 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران، کرج

3 گروه علوم باغبانی، دانشکده کشاورزی، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران، کرج

10.22059/ijfcs.2026.388811.655123

چکیده

گوجه‌فرنگی با نام علمی Solanum lycopersicum به صورت وسیعی در سرتاسر جهان در شرایط مزرعه و گلخانه کشت می‌شود. در سال‌های اخیر بذرهای هیبرید گوجه‌فرنگی به دلیل عملکرد بالاتر، تحمل تنش‌ها و مقاومت به بیماری‌ها مورد توجه زارعین واقع شده‌اند. بذر هیبرید که اشاره به بذر F1 دارد، به طور مستقیم از تلاقی دو والد که از لحاظ ژنتیکی کاملا از هم دور هستند، ایجاد می شود. اما یک ژنوتیپ هتروزیگوس نمی‌تواند به صورت پایداری از طریق بذر تکثیر شود زیرا در نسل F2 به دلیل تفرق صفات، هتروزیگوستی کاهش می‌یابد در نتیجه زارعین همه ساله مجبور به خرید بذر می‌باشند. در این پژوهش با کشت ده رقم از بذرهای هیبرید و تولید F2 و سپس انتخاب و خودگرده‌افشانی بین‌آن‌ها تا نسل F4 و در نتیجه تولید 52 تا لاین، جهت تشخیص حضور ژن‌های مقاومت به بیماری‌های گوجه‌فرنگی از جمله قارچ فیتوفترا (Phytophthora infestans)، ویروس موزاییک گوجه فرنگی (ToMV)، ویروس پیچیدگی برگ زرد (TYLCV) و ویروس پژمردگی لکه‌ای گوجه فرنگی (TSWV) با استفاده از پرایمرهای اختصاصی مورد بررسی قرار گرفتند. با توجه به نتایج‌های به دست آمده، مشخص شد که در 15 لاین (139، 107، 50، 125، 96، 105، 109، 106، 110، 58، 103، 112، 56، 101، 118) ژن‌های مقاومت به هر چهار نوع بیماری حضور دارند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Identification the Resistance Genes of Four Types of Important Diseases in Tomato Lines (Solanum lycopersicum L.)

نویسندگان [English]

  • Akram Esfandiar 1
  • Abdolhadi Hosein Zadeh 2
  • Mohammad Reza Naghavi 2
  • Reza Salehi Mohammadi 3
1 Department of Agronomy and Plant Breeding, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj,
2 Department of Agronomy and Plant Breeding, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj
3 Department of Horticultural Sciences, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj
چکیده [English]

Tomato, with the scientific name Solanum lycopersicum, is widely cultivated worldwide in both field and greenhouse conditions. In recent years, hybrid tomato seeds have attracted the attention of farmers due to their higher yield, stress tolerance, and disease resistance. Hybrid seed, which refers to F1 seed, is created directly from the crossing of two parents that are genetically completely distinct from each other. But a heterozygous genotype cannot be stably propagated through seed because heterozygosity is reduced in the F2 generation due to trait segregation., so farmers are forced to buy seed every year. In this research, ten varieties of hybrid seeds were cultivated and F2 was produced, followed by selection and self-pollination between them until the F4 generation, resulting in the production of 52 lines, These lines were examined using specific primers to detect the presence of resistance genes to tomato diseases, including Phytophthora infestans, Tomato Mosaic Virus (ToMV), Yellow Leaf Curl Virus (TYLCV), and Tomato Spotted Wilt Virus (TSWV). According to the results obtained, it was determined that in 15 lines (139, 107, 50, 125, 96, 105, 109, 106, 110, 58, 103, 112, 56, 101, 118), resistance genes to all four types of diseases are present.

کلیدواژه‌ها [English]

  • Tomato
  • Hybrid seed
  • Parent Lines
  • Resistance Genes
  • Virus
References:
Arens, P., Mansilla, C., Deinum, D., Cavellini, L., Moretti, A., and et al. (2010). Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness, uniformity and stability testing. Theoretical and Applied Genetics, 120, 655–664.
Ashish, P., Namisha, S., Gunaseelen, H., Mehanathan, M., & Manoj, P. (2020). Tomato yellow leaf curl virus: Impact, challenges, and management. Cell Press, 25(9), 897-911.
Ates, C., Fidan, H., Karacaoglu, M., & Dasgan, H. (2019). The identification of the resistance levels of Fusarium oxysporum f. sp. radicis-lycopersici and tomato yellow leaf curl viruses in different tomato genotypes with traditional and molecular methods. Applied Ecology and Environmental Research, 17(2), 2203-2218.
Carvalho, V.D. (1980). Chemical and industrial characterstics of tomato. Agricultural Report, Belo Horizonte, 6, 63-68.
Erik, W., Dun, K.V., Bastiaan de Snoo, C., Cilia, L.C., Lelivelt, J.B., Keurentjes, N.S., Ravi, M., Simon Chan, W.L., Jong, K., & Dirks, R. (2012). Reverse breeding in Arabidopsis thaliana generates homozygous parental lines from a heterozygous plant. Nature Geneticse, 44(4).
Farsi, M., & Bagheri, A. (1383). Principles of Plant Breeding. Jihad Daneshgahi Publications, Mashhad. (In Persian).
Gill, U., Scott, J.W., Shekasteband, R., Ogundiwin, E., Schuit, C., Francis, D.M., Sim, S.C., Smith, H., & Hutton, S.F. (2019). Ty-6, a major begomovirus resistance gene on chromosome 10, is effective against tomato yellow leaf curl virus and tomato mottle virus. Theoretical and Applied Genetics, 132(5), 1543-1554.
Grube, R.C., Radwanski, E.R., & Jahn, M. (2000). Comparative genetics of disease resistance within the solanaceae. Genetics, 155(2), 873-887.
Haya, K., Atul, G., & Sanjai, K. (2018). PCR-based methods for identification and detection of Phytophthora infestans in infected leaves of tomato. Defence Life Science Journal, 3(1), 41-44.
Heydarian, A., Olfati, J.A., Zakizadeh, H., & Rahimi Ajdadi, F. (2024). Tomato hybrid cv. Amaris phenotypic reverse breeding. Journal of Research in Horticultural Science, 2(2), 307-326.
Hull, R. (2009). Comparative plant virology, Academic press, p. Norwich, UK.
Ilias, D.A., Rafail, T., Ioannis, M., Ioannis, N.X., Athanasios, G.M., & Athanasios, G.M. (2021). Assessment of tomato recombinant lines in conventional and organic farming systems for productivity and fruit quality traits. Agronomy, 11(1). 129.
Joy, M., Rocky, M., Sultana, M., Kumkum, M., & Hossain, M.B. (2025). Evaluation of selected tomato cultivars effectiveness against tomato yellow leaf curl virus (TYLCV) and its PCR-based molecular detection. European Journal of Agriculture and Food Sciences.
Jungsu, J., Hyun Jung, K., Je Min, L., Chang, S.O., Hyung-Jin, L., & Inhwa, Y. (2015). Gene-based molecular marker system for multiple disease resistances in tomato against tomato yellow leaf curl virus, late blight, and verticillium wilt. Euphytica, 205, 599–613.
Kalloo, G. (1991). Genetic improvement of tomato, monographs on theoretical and applied genetics. Springer-Verlag, Berlin.1-9.
Klee, H.J., & Giovannoni, J.J. (2011).Genetics and control of tomato fruit ripening and quality attributes. Annual Review of Genetics, 45, 41-59.
Kumar, S., Udaya Shankar, A.C., Nayaka, S.C., Lund, O.S., & Prakash, H.S. (2011). Detection of tobacco mosaic virus and tomato mosaic virus in pepper and tomato by multiplex RT–PCR. Letters in Applied Microbiology, 0266-8254.
Kumar, M., Avinashe, H.A., Dubey, N., Ram, K., Kaur, S., & Kalubarme, S. (2019). Reverse breeding: Creating parental line for a heterozygous plant and its complication. Annals of Biology, 35(1), 50-54.
Nadeem, S., Ullah, N., Akhtar, K.P., Hameed, A., & Saleem, M.Y. (2022). Evaluation of tomato hybrids for resistance against tomato mosaic virus (ToMV). Journal of Botanical Research, 4(2).
Najeeb, U., Asad, A., Musharaf, A., Muhammd, F., Naseerud, D., & Fayaz, A. (2017). Evaluation of tomato genotypes against Tomato Mosaic Virus (ToMV) and its effect on yield contributing parameters. Pakistan Journal of Botany, 49(4), 1585-1592.
Raghuveer, S., Neelam, S., Angami, T., Touthang, L., & Kalita, H. (2025). Impact of late blight (Phytophthora infestans) on tomato yield and its environmental correlation. Indian Phytopathology, 78(5).
Sepat, N.K., Sepat, S.R., Sepat, S., & Kumar A. (2013). Energy use efficiency and cost analysis of tomato under greenhouse and open field production system at Nubra valley of Jammu and Kashmir. International Journal of Environmental Sciences, 3(4), 1233-1241.
Shekasteband, R., Samuel, F.H., & Jay, W.S. (2015). Designing new DNA markers and determining the effective size of Ph-2 and Ph-3 introgressions for late blight resistance stacking purposes in tomato. Research Reports, 65.
Shiming, Qi., Shijie, Zh., Monirul Islam, Md., Ahmed, H., El-Sappah, Zhang, F., & Liang, Y. (2021). Natural resources resistance to tomato spotted wilt virus (TSWV) in tomato (Solanum lycopersicum). International Journal of Molecular Sciences, 22, 10978.
Stevens, M., Scott, S., & Gergerich, R. (1991). Inheritance of a gene for resistance to tomato spotted wilt virus (TSWV) from Lycopersicon peruvianum Mill. Euphytica, 59(1), 9-17.
Supaporn, L., Lumpueng, R., & Orawan, C. (2005). Detection of tomato yellow leaf curl Thailand virus by PCR without DNA extraction. Molecular Biotechnology, 31(3), 233-8.

مقالات آماده انتشار، اصلاح شده برای چاپ
انتشار آنلاین از تاریخ 01 فروردین 1405
  • تاریخ دریافت: 06 بهمن 1403
  • تاریخ بازنگری: 22 دی 1404
  • تاریخ پذیرش: 10 بهمن 1404
  • تاریخ انتشار: 01 فروردین 1405