Responses of plants to salinity stress and development of salt tolerance are extremely complex and various mechanisms appear to be involved. We employed a proteomic approach to study the mechanism of plant responses to salinity in a sensitive cultivar of barley (527 line). Three-week-old seedlings were treated with 300 mM NaCl for 3 weeks. Total proteins of fourth leaf were extracted and separated by two-dimensional gel electrophoresis. 377 protein spots were reproducibly detected, including 55 that were up-regulated and 69 down-regulated. Using MALDI-TOF-TOF MS, 25 proteins involved in many cellular functions were identified. These proteins were; oxygen-evolving enhancer protein, methionine sulfoxide reductase, chloroplast RNA-binding proteins, chloroplast-localized cyclophilin, rubisco, ribulose bisphosphate carboxylase/oxygenase activase, Nascent polypeptide associated complex alpha, thioredoxin, dehydroascorbate reductase, oxalate oxidase-like protein or germin-like protein, profilin, universal stress proteins and ribosomal protein.
Fatehi, F., Hosseinzadeh, A., Alizadeh, H., Haji Abbasi, M., & Shabani, A. (2011). The Study of Proteomic Salinity Responses in Barley. Iranian Journal of Field Crop Science, 42(3), 617-626.
MLA
Foad Fatehi; Abdolhadi Hosseinzadeh; Houshang Alizadeh; Mahboubeh Haji Abbasi; Akbar Shabani. "The Study of Proteomic Salinity Responses in Barley". Iranian Journal of Field Crop Science, 42, 3, 2011, 617-626.
HARVARD
Fatehi, F., Hosseinzadeh, A., Alizadeh, H., Haji Abbasi, M., Shabani, A. (2011). 'The Study of Proteomic Salinity Responses in Barley', Iranian Journal of Field Crop Science, 42(3), pp. 617-626.
VANCOUVER
Fatehi, F., Hosseinzadeh, A., Alizadeh, H., Haji Abbasi, M., Shabani, A. The Study of Proteomic Salinity Responses in Barley. Iranian Journal of Field Crop Science, 2011; 42(3): 617-626.
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