How gene expression changes in four drought-sensitive and -tolerant genotypes under water deficit

Articles in Press

Document Type : Research Paper

Authors

1 Department of plant genetics and production engineering, Faculty of agriculture and natural resources, University of Mohaghegh Ardabili, Ardabil, Iran.

2 Department of Agricultural Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran.

3 Department of Agronomy and plant breeding, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran.

Abstract

Drought stress is one of the most important limiting factors for plant growth and soil fertility, which causes crop yield reduction. In order to investigate the relative expression of genes related to drought stress tolerance, 4 genotypes (including two sensitive and two resistant to moisture stress) of bread wheat as factor A were subjected to a factorial experiment on basis randomized complete block design with three replications under normal and drought stress at the beginning of flowering stage as factor B in the research farm of Azerbaijan Shahid Madani University. The expression of genes in the examined samples was performed by semi-quantitative polymerase chain reaction in three replicates. Glutathione peroxidase gene (GAPDH) was used as housekeeping gene. Examining the relative expression level of antioxidant genes showed that the high tolerance of Mihan cultivar to drought stress can probably be attributed to the high activity of catalase and ascorbate peroxidase enzymes and the high tolerance of Arum cultivar to the higher activity of antioxidant enzymes proline synthetase, peroxidase and attributed to superoxide dismutase. Considering that the antioxidant genes catalase, ascorbate peroxidase, proline synthetase, peroxidase and superoxide dismutase in tolerant cultivars showed a significant increase in expression under stress and normal conditions compared to sensitive cultivars under the same stress and normal conditions, so this Genes may induce tolerance to drought stress in plants under stress conditions.

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Main Subjects

Iranian Journal of Field Crop Science

Articles in Press, Accepted Manuscript
Available Online from 13 April 2024

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History

  • Receive Date: 18 September 2023
  • Revise Date: 10 November 2023
  • Accept Date: 13 November 2023
  • Publish Date: 13 April 2024

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