Investigating the physiological response of plant leaf tissue (Ammi majus L.) to melatonin under heat stress

Document Type : Research Paper


1 Department of Agricultural Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

2 Department of Agricultural Biotechnology, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran.


Traditional medicine has been successful in treating chronic diseases and related problems. Considering the very high importance of medicinal plants and the growing need for the cultivation of these plants, especially Ammi majus plant due to its very effective medicinal compounds, it seems that the use of external stimuli in order to increase the quantity and The quality of secondary metabolites can be effective as an auxiliary method in the cultivation of this plant. This plant is thermophilic and tolerates heat to some extent. In this research, different levels of melatonin (0, 30, 60 ppm) and time intervals of 24 and 48 hours were used. The results of this research showed that the use of different levels of melatonin, especially the concentration of 60, effectively prevented the destruction of the pigments of plants under heat stress, and even their amount increased approximately 2 times at 60 ppm compared to 0 concentration. At the same time, melatonin increased the activity of antioxidant enzymes and thus led to a decrease in the content of reactive oxygen species that were increased under heat stress.The amount of malondialdehyde has also decreased twice as a result of melatonin spraying, especially 60 ppm. The result was the reduction of free radicals, reduction of electrolyte leakage, malondialdehyde content, and finally, the increase of tolerance of plants under heat stress.Generally, it can be concluded that melatonin as a functional stimulant is capable of stimulating physiological changes in plants treated with heat with the aim of increasing their tolerance


Main Subjects

Articles in Press, Accepted Manuscript
Available Online from 13 April 2024
  • Receive Date: 29 August 2023
  • Revise Date: 03 October 2023
  • Accept Date: 06 January 2024
  • Publish Date: 13 April 2024