Effect of hormone spraying and bacterial inoculation on bean plant under different temperature and humidity conditions

Document Type : Research Paper


1 PhD. student of Agronomy department, Faculty of Agriculture & natural sciences,, University of Tehran

2 Agronomy and Plant Breeding Department, Faculty of Agriculture,, University of Tehran, Karaj, Iran.


To investigate the effect of acetylsalicylic acid (ASA) and Bacillus velezensis bacteria on the yield and yield components of it, an experiment was conducted in the form of split-split plots in the form of a randomized complete block design in four replicates in the research farm of the Faculty of Agriculture and Natural Resources, University of Tehran in 2021, it was done on separate planting dates (Mid May and late June). The treatments included two irrigation levels (irrigation 70% and 100% of the plant's water requirement) as the main plot, foliar spraying (ASA hormone at 0.5 mM level and control) as a secondary plot and inoculation with Bacillus velezensis bacteria and no inoculation as a secondary subplot. Results of the analysis of variance showed that the interactions of sowing date, irrigation, foliar spraying, and inoculation with bacteria on the traits measured in pinto beans was significant. The maximum plant height (154 cm), number of sub-branches (7), leaf area index (3.7), seed yield and harvest index (3045 kg/ha and 43%, respectively), 100 seed weight (37 grams), pod numbers (24) were obtained in mid-May planting date, along with salicylic acid foliar spraying and bacterial inoculation without water stress. Water deficit stress (70% of plant water requirement) and foliar spraying with ASA hormone and inoculation with bacteria, in late June sowing date the highest biological yield (7997 kg/ha) obtained. Overall, hormone spraying and inoculation with bacteria improved the yield characteristics of pinto bean under drought-stress conditions in both sowing dates.


Main Subjects

Articles in Press, Accepted Manuscript
Available Online from 13 April 2024
  • Receive Date: 28 May 2023
  • Revise Date: 08 April 2024
  • Accept Date: 19 November 2023
  • Publish Date: 13 April 2024