The impact of temperature on metabolites and the expression of genes involved in thymol and carvacrol biosynthesis pathway in Thymus vulgaris

Document Type : Research Paper

Authors

1 Agronomy and Plant Breeding Dept. University of Tehran

2 null

Abstract

Environmental factors make changes in the amount and type of secondary metabolites. To study the effects of temperature regimes on secondary metabolites and the expression of genes involved in biosynthesis pathway of thymol and carvacrol in thyme, an experiment was conducted in a growth chamber for one month with three thermal treatments including heat, cold and normal temperatures. Essential oil compounds were measured by Gc / MS and Gc and the expression of TvTps1, CYP178 and CYP180 genes assayed by qRT-PCR. Both heat and cold temperatures increased the phenolic monoterpen compounds (thymol and carvacrol) compared to normal condition. High temperature led to significant increases in carvacrol while low temperature resulted in a great increase in the amount of thymol. Gamaterpenin level was declined due to thermal treatments. Gene expression analysis revealed that the cold condition increased the expression of TvTps1 and CYP178 genes, while heat decreased the expressions. CYP180 and CYP181 were highly expressed under both cold and heat conditions, although the amount of expression was much higher under high temperature. Generally, the results indicated that the environment temperature played a key role in thyme metabolites type and content. It could be concluded that the regions with high and low temperatures were suitable for carvacrol and thymol production, respectively.

Keywords

References

  1. REFERENCES

    1.  Burke, C. C., Wildung, M. R. & Croteau, R. (1999). Geranyl diphosphate synthase: Cloning, expression, and characterization of this prenyltransferase as a heterodimer. Proceedings of the National Academy of Sciences, 9,13062–13067.
    2. Channuntapipat, C., Sedgley, M. & Collins, G. (2001). Sequences of the cDNAs and genomic DNAs encoding the S1, S7, S8, and Sf alleles from almond, Prunus dulcis. Theoretical and Applied Genetics, 103, 1115-1122.  
    3. Clark, J. & Menary, R. C. (1980). Environmental effects on peppermint (Mentha. piperita L) I. Effect of day length, photon flux density, night and day temperature on yield and composition of peppermint oil. Austral. J. Plant Physiol, 7, 685-692.
    4. Crocoll, C. (2011). Biosynthesis of the phenolic monoterpenes, thymol and carvacrol, by terpene synthases and cytochrome P450s in oregano and thyme. PhD Dissertation. Biologisch Pharmazeutischen Fakultät der Friedrich-Schiller-Universität Jena, Germany.
    5. Dudai, N., Putievsky, E., Palevitch, D & Halevy, A. (1992). Monoterpene content in Origanum syriacum as affected byenvironmental conditions and flowering. Physiologia Plantarum, 84, 453-459.
    6. Duriyaprapan, S., Britten, E. J. & Basford, K. (1986). The effect of temperature on growth, oil yield and oil quality of Japanese mint. Annals of Botany, 58, 729-736.
    7. Eguchi, Y., Widiastuti, A., Odani, H., Chinta, Y. D., Shinohara, M., Misu, H., Kamoda, H., Watanabe, H. & Hasegaw, M. (2016). Identification of terpenoids volatilized from Thymus vulgaris L. by heat treatment and their in vitro antimicrobial activity. Physiological and Molecular Plant Pathology, 94, 83-89.
    8. Fakhr Tabatabai, S. M. (2006). A systematic encounter with living nature and other articles in the field of ecology. Anteshar Co., Tehran, 186 Pp.
    9. Fletcher , R., Slimmon, T., Auley, S. & Kott , L.  (2005). Heat stress reduces the accumulation of rosmarinic acid and the total antioxidant capacity in spearmint (Mentha spicata L). Journal of the Science of Food and Agriculture, 85, 2429–2436.
    10. Gershenzon, J. & Dudareva, N. (2007). The function of terpene natural products in the natural world. Nature Chemical Biology, 7,408-414.
    11. Gröger, S., Schmiderer, C., Steinborn, R. & Novak, J. (2012). Seasonal influence on gene expression of monoterpene synthases in Salvia officinalis (Lamiaceae). Plant  Physiology, 169, 353-359.
    12. Habibi, S., Qaderi, A. & Fatehi, F. (2017).  The Study of relative expression of key genes of thymol biosynthesis pathway in Thymus vulgaris cv. ‘Varico 3’ under Cold Stress Using Real-Time PCR. Journal of Medicinal plants, 16 (4), 50-59.
    13. Jamzad, Z. (2009). Thymus  and Satureja  species of  Iran. Research Institute of Forests and Rangelands, Tehran, Iran. (In Persian)
    14. Kokkini, S., Karousou, R., Dardioti, A., Krigas, N. & Lanaras, T. (1997).  Autumn essential oils of Greek oregano. Phytochemistry, 44, 883- 886.
    15. Maffei, M. & Scannerini ,S.  (2011). Photomorphogenic and chemical responses to blue light in Mentha piperita. Journal of Essential Oil Research, 72, 712-718.
    16. Mondak, B., Mohammadi, V. A., Zeinali, H. & Hadian, J. (2015). Evaluation of genetic variability in Iranian Thymus daenensis sub sp. daenensis, by use of inter simple sequence repeat (ISSR) markers. Journal of modern genetic, 10(4), 575-584.
    17. Morshedloo, M. R., Craker, L. E., Salami, A., Nazeri, V., Sang, H. & Maggi, F. (2017). Effect of prolonged water stress on essential oil content, compositions and gene expression patterns of mono- and sesquiterpene synthesis in two oregano (Origanum vulgare L.) subspecies. Plant Physiology and Biochemistry, 111, 119-128.
    18. Nickavar, B., Mojab, F. & Dolat-Abadi, R. (2005). Analysis of the essential oils of two Thymus species from Iran. Food Chemistry, 90, 609–611. (In Persian)
    19. Novak, J., Lukas, B. & Franz, C.  (2010). Temperature influences thymol and carvacrol differentially in Origanum spp. (Lamiaceae). Journal of essential oil research, 22, 412-418.
    20. Omid baigi, R. (2009). Product and processing of medicinal plant. Astan ghods razavi press. Mashhad, Iran. (In Persian)
    21. Pateraki, A. & Kanellis, K. (2010).  Stress and developmental responses of terpenoid biosynthetic genes in Cistus creticus subsp. creticus, Plant Cell Report, 29, 629- 641.
    22. Pfaffl, M. W. (2001). A new mathematical model for relative quantification in real-time RT–PCR. Nucleic acids research, 29(9), e45. doi: 10.1093/nar/29.9.e45.
    23. Rudolph, K., Parthier, C., Egerer-Sieber, C., Geiger, D., Muller, Y. A., Kreis, W. & Müller, F.  (2016). Expression, crystallization and structure elucidation of γ-terpinene synthase from Thymus vulgaris. Acta Crystallogr, 72, 16-23.
    24. Sangwan, N., Farooqi, A. H., Shabih, F. & Sangwam, R. (2001). Regulation of essential oil production in plants. Plant Growth Regulation, 34, 3-21.
    25. Schellmann, S. & Hülskamp, M. (2005). Epidermal differentiation: trichomes in Arabidopsis as a model system. The International Journal of Developmental Biology,49 (5-6), 579-584.
    26. Stahl-Biskup, E. & Saéz, F. (2002). Thyme: the genus Thymus, medicinal and aromatic plant-industrial profiles, Taylor & Francis, London.
    27. Thompson, J., Chalchat, J. E., Michet, A., Linhart, Y. & Ehlers, B. (2003).  Qualitative and quantitative variation in monoterpene co-occurrence and composition in the essential oil of Thymus vulgaris chemotypes. Journal of Chemical Ecology, 29, 859-880.
    28. Timmermans, K. (2003). Intellectual property rights and traditional medicine: policy dilemmas at the interface. Social Science & Medicine, 57,745-756.
    29. Yanivie, Z. & Palevitch, D. (1982). Effects of drought on secondary metabolites of medicinal and aromatic plants. In: Atal CK, Kapur BM (eds) Cultivation and Utilization of Medicinal Plant. Regional Research Laboratory council of Scientific & Industrial Research, Jammu-Tawi, pp.1–12.
    30. Davis,E. M. & Croteau, R. (2000). Cyclization enzymes in the biosynthesis of monoterpenes, sesquiterpenes, and diterpenes. Topics in Current Chemistry, 209, 53–95.
    31. Lambert, E., Faizal, A. & Greelen, D. (2011). Modulation of triterpene saponin production: in vitro cultures, elicitation, and metabolic engineering. Applied Biochemistry and Biotechnology, 164, 220-237.
Iranian Journal of Field Crop Science
Volume 51, Issue 1
April 2020
Pages 195-205

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History

  • Receive Date: 21 October 2018
  • Revise Date: 27 February 2019
  • Accept Date: 06 April 2019
  • Publish Date: 20 April 2020

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