Iranian Journal of Field Crop Science

Iranian Journal of Field Crop Science

Increasing symbiotic nitrogen fixation in soybean by using mutation in microsymbiont Bradyrhizobium japonicum

Document Type : Research Paper

Authors
Javad Pirvali Beiranvand 1
Ahmad Ali Pourbabaee 2
Hossein Ali Alikhani 3
Sayed Pejman Shirmardi 4
Alireza Abbassi 5
Babak Motesharezadeh 6
1 Nuclear science and Technology Reseach Institute, Atomic Energy Organization of Iran, Tehran, Iran, P. O. Box: 31485-498
2 Dept. of soil science, university of Tehran
3 scientific member
4 Asistance prof. Nuclear science and Technology Reseach Institute, Atomic Energy Organization of Iran
5 associate professor
6 associate prof., colleage of Agriculture and Natural Resources, University of Tehran.
10.22059/ijfcs.2018.241539.654376
Abstract
To increase the symbioticnitrogen fixation in soybean, a native Bradyrhizobium japonicum strain RS 117 was selected from four strains via infectiveness, symbiotic effectiveness and sequancing 16S rRNA gene. Then, mentioned strain in the form of YMB inoculum wasirradiated by gamma rays dosages 0-5000 Gray with a 500 Gray interval dosage. About 800 mutant isolates were collected on YMA from different doses. 11 isolates in efficiency point of view changing the GMGT media colour were selected as the best isolates for further study. These isolates were tested for their symbiotic effectiveness (SE) under proper greenhouse condition with a randomized complete block design in 3 replications; and 3 more efficient isolates No. 3, 8 and 9  increased 56- 81 percent SE more than wild strain. At last, relative gene expersion nodA, nifK2 in mutant and wild strain bacteria approved mollacular differences between both of them by indicating Increase and decrease in amount, in mutant isolate number 9 in comparison with the wild Bradyrhizobium japonicum strain RS 117. The results indicate that gamma-irradiated mutations in Bradyrhizobium japonicum strains have significantly increased the biological fixation of nitrogen in soybean plants.
Keywords
symbiotic nitrogen fixation
gamma irradiation
nodA
nifK2
relative gene expression

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Iranian Journal of Field Crop Science
Volume 50, Issue 3
Autumn 2019
Pages 135-148

  • Receive Date 17 September 2017
  • Revise Date 12 August 2018
  • Accept Date 14 August 2018
  • Publish Date 23 October 2019