The effect of abiotic elicitors on antioxidants and phytochemical traits of celandine (Chelidonium majus) under drought stress

Document Type : Research Paper

Authors

1 Department of Plant Breeding, Imam Khomeini International University, Qazvin, Iran

2 M.Sc. Student, Imam Khomeini International University, Qazvin, Iran

Abstract

This experiment was carried out to investigate the effect of methyl jasmonate and salicylic acid on the antioxidant production and physiological-biochemical properties of Chelidonium majus under drought stress condition. For this, an experiment was conducted as factorial based on completely randomized design with four replications in Imam Khomeini International University during 2016-2017. Three factors included drought stress in two levels (non-stress and drought stress with 100% and 50% field capacity irrigation, respectively), elicitor in three levels (control, 100 μm methyl-jasmonate and 2 mM salicylic acid) and plant organs (root, stem and leaf). Analysis of variance and mean comparisons showed that methyl-jasmonate and salicylic acid reduced the activity of CAT enzyme. Both salicylic acid and methyl-jasmonate did not affect the activity of SOD, but methyl-jasmonate increased the activity of GPX and salicylic acid reduced APX activity under drought stress. Also, methyl-jasmonate and salicylic acid reduced APX activity compared to control treatment. The highest amount of protein content was observed in root and then in leaf. Salicylic acid increased total protein content up to 34% compared to control treatment. Both Methyl-jasmonate and salicylic acid increased chlorophyll chlorophyll, carotenoids, flavonoids and anthocyanin compared to control treatment, but salicylic acid was superior in increasing of chlorophyll and carotenoids whereas methyl-jasmonate was superior in increasing of flavonoids and anthocyanin. In conclusion, methyl-jasmonate and salicylic acid have increased the ability of celandine in response to drought stress, and also, in addition to the protective effect against drought-induced oxidation, they increased photosynthetic pigments and some of metabolites.

Keywords

Main Subjects

References

  1. Arnon, D.I., 1949. Copper enzymes in isolated chloroplasts; polyphenol-oxidase in Beta vulgaris.
  2. Plant Physiol. 24, 1-15.
  3. Agarwal, S. & Pandey, V. (2004). Antioxidant enzyme responses to NaCl stress in Cassia angustifolia. Plant Biology, 48, 555-560.
  4. Alfonso, L. V. & Martin-Mex, R. (2007). Effect of salicylic acid on the bioproductivity of plants. Springer, 15-23.
  5. Amiri, A., Sirous-Mehr, A. R., Yadollahi, P., Asgharpur, M. R. & Ismail Zadeh Bahabadi, S. (2016). Effect of drought stress and spraying salicylic acid and chitosan on photosynthetic pigmentation and antioxidant enzymes of safflower. Journal of Crop Agricultural, 18(2), 453-466. (In Farsi)
  6. Asadi Kavan, Zh., Ghorbanli, M. & Sateei, A. (2010). The effect of drought stress and exogenous ascorbate on photosynthetic pigments, flavonoids, phenol compounds and lipid peroxidation in Pimpinella anisum L. Iranian Journal of Medicinal and Aromatic Plants, 25(4): 456-469.
  7. Bayan, M., Amini, F. & Askari, M. (2014). Effect of salicylic acid on organic osmolites accumulation and antioxidant activity of Nitraria shoberi L. under drought stress Conditions. Journal of Plant Production, 20 (4): 177-188. (In Farsi)
  8. Bowler, C., Van Montagu, M. & Inze, D. (1992). Superoxie dismutase and stress tolerance. Annual review of plant physiology and plant molecular biology, 43, 83-116.
  9. Chen, G. X. & Asada, K. (1989). Ascorbate peroxidase in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular propertyes. Plant Cell Physiology, 30, 987–998.

10. Creelman, R.A., Tierney, M.L. & Mullet, J.E. (1992). Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression. Proceedings of the National Academy of Sciences. 89: 4938-4941.

11. Dragana Jakovljevic, D., Milan Stankovic, S. & Marina Topuzovic, D. (2013). Seasonal variability of Chelidonium majus L. secondary metabolites content and antioxidant activity. Experimental and Clinical Sciences, 12, 260-268.

12. Egert, M. & Tevini, M. (2002). Influence of drought on some physiological parameters symptomatic for oxidative stress in leaves of chives (Allium schoenoprasum). Environmental and Experimental Botany, 48, 43-49.

13. El-Tayeb, M. A. (2005). Response of barley grain to the interactive effect of salinity and salicylic acid. Plant Growth Regulation, 45, 215-225.

14. Esfandiari, A., Tajik, T., Shakerpour, M. & Firoozabadi, M. (2010). The destructive effects of active oxygen species on cell defense ability with increasing age of leaves in wheat. Production of Crops, 3, 219-227. (In Farsi)

15. Eskandari-Zanjani, K., Shiranirad, A. M., Moradi-Aghdam, A. & Taherkhani, T. (2012). Effect of salicylic acid application in salt stress conditions on physiological and morphological characteristics of Artemisia (Artemisia annua L.). Ecophysiology of Crop Plants, 4(24), 428-415. (In Farsi)

16. Fabriki-Ourang, S. & Mehrabad-Pourbenab, S. (2016). Study of drought and salinity effect on morphological and biochemical characteristics on satureja hortensis L. Echophytochimistry of Medicinal Plants. 15(4), 23-35. (In Farsi)

17. Fathi, GH., Esmaeilpour, B. & Jalilvan, P. (2015). Plant growth regulator (principles and application). Mashhad Jahadeh Daneshgahi Press. (In Farsi)

18. Ghai, N., Setia, R. C. & Setia,  N. (2002). Effect of paclobutrazol and salicylic acid on chlorophyll content, hill activity and yield components in Brassica napus L. Phytomorphology, 52, 83-87.

19. Hausladen, A., Alscher, R.G. (1993). Glutathione. In: R.G. Alscher, J.L. Hess, (Ed), Antioxidants in higher Plants. (pp. 1–30.) Boca Raton, CRC Press.

20. Horvath, E., Pal, M., Szalai, G., Paldi, E. & Janda, T. (2007). Exogenous 4-hydroxybenzoic acid and salicylic acid modulate the effect of short-term drought and freezing stress on wheat plants. Biologia Plantarum, 51:480-487.

21. Horvath, E., Szalai, G. & Janda, T. (2000). Induction of abiotic Stress tolerance by salicylic acid signaling: Review. Plant Growth Regulation, 26, 290-300.

22. Jafarzadeh, L., Omidi, H. & Bustani, A. (2013). The effect of drought and nitrogen biofertilizer on some biochemical characteristics of spring flower. Journal of Iranian Biology, 2(27), 180-193. (In Farsi)

23. Jung, S. (2004). Effect of chlorophyll reduction in Arabidopsis thaliana by methyl jasmonate or norflurazon on antioxidant systems. Journal of Plant Physiology and Biochemistry, 42, 231-255.

24. Kang, G. Z., Wang, C. H., Sun, G. C. & Wang, Z. X. (2003). Salicylic acid changes activities of H2O2- metabolizin.g enzymes and increases the chilling tolerance of banana seedlings. Environmental and Experimental Botany, 50, 9-15.

25. Kaydan, D., Yagmur, M. & Okut, N. (2007). Effects of salicylic acid on the growth and some physiological characters in salt stressed wheat (Triticum aestivum L.). Tarim Bilimleri Dergisi, 13(2), 114-119.

26. Lawlor, D. W. & Cornic, G. (2002). Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant Cell and Environment, 25, 275-294.

27. Niakan, M. & Zanganeh, A. (2015). Effect of drought stress and salicylic acid on the activity of antioxidant enzymes in Fenugreek.  Iranian Plant Ecophysiological studies, 33(1): 38-45. (In Farsi)

28. Petrov, V. D. & Breusegem, F. V. (2012). Hydrogen peroxide-a central hub for information flow in plant cells. Cell Biology, 14, 1093-1206.

29. Ramroudi, M. & Khamar, A. R. (2013). Interaction effects of salicylic acid solution and irrigation treatments on some quantitative, qualitative and osmotic parameters of basil. Applied Research of Plant Ecophysiology, 1(1), 19-32.

30. Rauf-Fard, F.,  Sharifi,  M., Omidbeigi,  R., Sefidkon, F., Bahmanesh, M. & Ahmadi, N. (2014). The Effect of methyl-jasmonate on metabolism enzymes and phenolic materials in agustact drug. Iranian Journal of Medicinal Plants and Herbs Research, 30(3), 369-361. (In Farsi)

31. See, K. S., Bhatt, A. & Keng, C. L. (2011). Effect of sucrose and methyl jasmonate on biomass and anthocyanin production in cell suspension culture of Melastoma malabathricum. International Journal of Tropical Biology and Conservation, 59(2), 597- 606.

32. Senaratna, T., Touchell, D., Bunn, E. & Dixon, K. (2000).  Acetyl salicylic acid (Asprin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regulation, 30, 157-161.

33. Shabani, L. & Ehsanpour, A. (2009). Induction of antioxidant enzymes, phenolic compounds and flavonoids in cultivation in licorice using methyl-jasmonate and salicylic acid. Iranian Biology, 22(4), 691-703. (In Farsi)

34. Tsuchiya, T., Ohta, H., Okawa, K., Lwamatsu, A., Shimada, H., Masuda, T. & Takamiya, K. I. (1995). Cloning of chlorophyllase, the key enzyme in chlorophyll degradation: finding of a lipase motif and the induction by methyl jasmonate. Proceedings of the National Academy of Sciences, 96(26), 15362-15367.

35. Upadhyaya, A., Sankhla, D., Davis, T. D., Sankhla, N. & Smith, B. N. (1985). Effect of paclobutrazol on the activities of some enzymes of activated oxygen metabolism and lipid peroxidation in senescing soybean leaves. Journal of Plant Physiology, 121, 453-461.

36. Yu, L.J., Lan, W.Z., Qin, W.M. & Xu, H.B. (2001). Effects of salicylic acid on fungal elicitor induced membrane-lipid peroxidation and Taxol production in cell suspension cultures of Taxus chinensis. Process Biochemistry.37:477-82.

37. Yu, Z. Z., Fu, C., Han, Y. S., Li, Y. X. & Zhao, D. X. (2006). Salicylic acid enhances jaceosidin and syringin production in cell cultures of Saussurea medusa. Biotechnology Letter, 28, 1027-1031.

Iranian Journal of Field Crop Science
Volume 50, Issue 1
May 2019
Pages 139-150

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History

  • Receive Date: 15 January 2018
  • Revise Date: 03 June 2018
  • Accept Date: 09 June 2018
  • Publish Date: 22 May 2019

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